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Reques PA14NR Set
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Library Description

Version 1.0 of the PA14NR Set is a subset of approximately 5800 mutants from the PA14 parental library, in which a single mutant with a transposon insrted in a particular gene has been chosen to represent that gene. Each mutant in the PA14NR Set has been colony purified during library assembly to minimize cross-contamination. The PA14NR Set growth conditions were optimized to keep phenotypic variant population sizes at a minimum. The PA14NR Set is ordered in a convenient 96-well format (approximately 65 96-well plates) for easy screening either with or without the use of automation equipment. Furthermore, the majority of the mutants in the library are TraSH-compatible allowing users to monitor the absence and presence of pooled mutants under various test conditions.

Current Availability

The Ausubel lab will no longer directly distribute the library. The library will now be available for distribution by Lael Yonker, MD and Bryan Hurley, PhD of the Mucosal Immunology and Biology Research Center at MGHfC.

If you are interested in obtaining a copy of the library, please send an email to .

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