Mutant Report for Mutant ID: 56320
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 30865
Chromat Path: H:\Pa14\MrT7\587v1\587v1_F04_F4_041.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTACACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATTTCC
CCTCCAACCTGATCGANGACCTCTACCTCAGCAACGGCATGAGCGCCGGTAATACCCTGGCCAAACATCA
TCTGCAGGCCTGGCCACTCAACCCTATTTGGGTCANNNNNNNNNNNNNNNNNNTNNTTTTTTTTTTTTTT
TTTTTCCCCCTTTTCCTTTAATCTCCCTTCTTTTTTTTTTTTTTTTTTTCTCCCTTTTTTTCCTTCTCTC
TTTCTTCTTCTCTCTTTTTTCTTTCTTTTTTTCTTTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT
TTTTTCTCTTTTTTATTTTCTTTCCTTTGCCCTCTCCCTCCTCTTTTTTTCTTCTTTTTTTTTTTCTTTT
TCTTCTTTGTCTGCGCCTCTTGCTTCTTCTGTTTCTGTTTTGGTTTCCGTTGTGTGTCGCTACTTTCGTT
GGCTGTCTTTTGTGGTGTGTTTGTTCTTGTAGTATTGATTTGCTGGTTTGTTGTTTGTATTTTTGTTGTT
TTTTGCTTTTGGAGGGAGGTTGTTAAGCGGTTTTTTGTGGTGGTGTGTGTGTTTGTTCCCTGTTTTTTGC
GTTCTTCCGCTTGTTAGATTTGTGTTTAAATTATATGTGTTTTTTTATTTTATATGTGCTTCTTTTCCTC
GTCCCTTTGCACCCTGATGTTCGCTTTTTTCGGATTTTTCTGTTTTTTTTTCATGGCTGTCCGCCCTGGT
CCAGAGTTTGTGAGTTTTTTTGTGAGAACTTTATTGGTGGGTAGGGCTGTGTTTTGGTTGTGTTGTTAGT
ATGCGCCGAATGCATTTTGTAGTATTTTTGTTTTATTTTGTGATGCCTTTCTGGACTT
Blast against PA14
Processed Seqquence ID: 23715
5' Trim Position: 14 3' Trim Position: 257
Processed Sequence:
(Transposon Sequence in Red)		 
CGACTACACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATTTCCCCTCCAACCTGATC
GANGACCTCTACCTCAGCAACGGCATGAGCGCCGGTAATACCCTGGCCAAACATCATCTGCAGGCCTGGC
CACTCAACCCTATTTGGGTCANNNNNNNNNNNNNNNNNNTNNTTTTTTTTTTTTTTTTTTTCCCCCTTTT
CCTTTAATCTCCCTTCTTTTTTTTTTTTTTTTT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
103928 103928 103929 103929
PA14 BLAST Hit ID: 103928       Show Transposon Map
Identities: 67/69 (97.10%) BLAST Bit Score: 123.0 Percent of Processed Sequence That Aligns: 27.98%
Query Start: 50 Query End: 118 Subject Start: 3527216 Subject End: 3527148 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3527216 PA14_39600*,PA14_39610 -,+
Raw Sequence Start: 3527279 PA14_39600*,PA14_39610 -,+
Extrapolated Insert Location: 3527215 PA14_39600*,PA14_39610 -,+
Default Offset Location: 3527216 PA14_39600*,PA14_39610 -,+
PAO1 BLAST Hit ID: 103929       Show Transposon Map
Identities: 66/69 (95.65%) BLAST Bit Score: 115.0 Percent of Processed Sequence That Aligns: 27.98%
Query Start: 50 Query End: 118 Subject Start: 2105116 Subject End: 2105184 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2105116 PA1926 +
Raw Sequence Start: 2105053 PA1926 +
Extrapolated Insert Location: 2105117 PA1926 +
Default Offset Location: 2105116 PA1926 +
H PA14 Blast Hit H PAO1 Blast Hit