Mutant Report for Mutant ID: 56031
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 30576
Chromat Path: H:\Pa14\MrT7\584v1\584v1_F03_F3_029.ab1 Last Transposon Base: 58 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
CGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATGGCCCGCGAA
TATTCCTTGGACTAATTTCCGACAAACCGGAAAAACCCATCCATAGGGGAAGTTAAGAGCAGCTCTTCTG
CCCTTCTGCTCTTCTGAGCTTCAGTCGGATAAGGAGCTGGAATGTTTATCGAGGCCGCCATAGTCATTTA
CGCTCTGGAACTCGATATTTCCAGAAACGCCGGGGGGAGTTCCCTTGATTTCGAATCGGCGACTCGACTT
CGGCGCGACCATGTCGGCATCAACGACATAGGAGGCATTGCCAGCTTTTAATTTCACGTTGGCGAAAGAG
ACATGAAACGCAGTATCGTTGGAAACTTCGACAACCGACTTACCTCCCTGCCGAACAAGACGCCAGATCA
ACTTTCCAGGCGCTTCGGCGGAAGTTCCCGACAAGTTATCGGGGCGGTAGAAGACCTTGATACGCTGGCG
CAACATCATCTGCAAGCCCTGGCCATTGNNCCCTNTTTNCCGGGTGTCCTTCTCTTTCCCTGGGGCCTTC
TTTCTCTTTGTATTCGTGTGGGTTCGTATTTTTTACTCTTCTCCTCTTTTATTTCTTCTTTCTTATTTAC
TCTTTTTCTATCGTCTCCCTGCGTCTTGTTTCAGTTATACGATTTTCTCTATCTTTTTTTTCCCCCTTTA
TCTGTGTCTATATTTCCGGTTATTCACATACCTCTACCAGTTCTTTGCTTAGCGTCGCATGCTTGTGATT
GTATGGTGCTTATTGTCTTTCTACCTGATCGTTTTGAGCGAGTTCCTCACCCACACACTACATCTCTTCA
ACCCTTTCCTGCCCACCTACGCCGACTCTCACCGCGCCTACTCTTCCGGCGGCGACTTTGG
Blast against PA14
Processed Seqquence ID: 23476
5' Trim Position: 7 3' Trim Position: 507
Processed Sequence:
(Transposon Sequence in Red)		 
ATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATGGCCCGCGAATATTCCT
TGGACTAATTTCCGACAAACCGGAAAAACCCATCCATAGGGGAAGTTAAGAGCAGCTCTTCTGCCCTTCT
GCTCTTCTGAGCTTCAGTCGGATAAGGAGCTGGAATGTTTATCGAGGCCGCCATAGTCATTTACGCTCTG
GAACTCGATATTTCCAGAAACGCCGGGGGGAGTTCCCTTGATTTCGAATCGGCGACTCGACTTCGGCGCG
ACCATGTCGGCATCAACGACATAGGAGGCATTGCCAGCTTTTAATTTCACGTTGGCGAAAGAGACATGAA
ACGCAGTATCGTTGGAAACTTCGACAACCGACTTACCTCCCTGCCGAACAAGACGCCAGATCAACTTTCC
AGGCGCTTCGGCGGAAGTTCCCGACAAGTTATCGGGGCGGTAGAAGACCTTGATACGCTGGCGCAACATC
ATCTGCAAGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
103137 103137 103138 103138 103139 103139
PA14 BLAST Hit ID: 103137       Show Transposon Map
Identities: 429/429 (100.00%) BLAST Bit Score: 850.0 Percent of Processed Sequence That Aligns: 85.60%
Query Start: 58 Query End: 486 Subject Start: 4575148 Subject End: 4575576 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4575148
Raw Sequence Start: 4575084 PA14_51450 -
Extrapolated Insert Location: 4575142 PA14_51450 -
Default Offset Location: 4575147 PA14_51450 -
PAO1 BLAST Hit ID: 103138       Show Transposon Map
Identities: 341/349 (97.70%) BLAST Bit Score: 628.0 Percent of Processed Sequence That Aligns: 69.60%
Query Start: 138 Query End: 486 Subject Start: 1074689 Subject End: 1074341 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1074689
Raw Sequence Start: 1074833 PA0994 -
Extrapolated Insert Location: 1074775
Default Offset Location: 1074770
PAO1 BLAST Hit ID: 103139       Show Transposon Map
Identities: 85/88 (96.59%) BLAST Bit Score: 151.0 Percent of Processed Sequence That Aligns: 17.40%
Query Start: 58 Query End: 145 Subject Start: 1074784 Subject End: 1074697 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1074784
Raw Sequence Start: 1074848 PA0994 -
Extrapolated Insert Location: 1074790 PA0994 -
Default Offset Location: 1074785 PA0994 -
H PA14 Blast Hit H PAO1 Blast Hit