Mutant Report for Mutant ID: 42715
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 26476
Chromat Path: H:\Pa14\MrT7\445v1\445v1_H07_H7_062.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTCACTATAGGGTGTCTAGAGACCGGAGACTTATCAGCCAACCTGTTAGTCGG
TATTCAGGCGCGGGCCGCTGGAGCAGCCGGCCAGCAGCGACAGGGTGAAAGCCAGTAGAACGAACGAACG
CATGATGACTCCGAAAAAAATGGGCAGCGCGGTCAGGCGGCGATGTGCAACACGCTGCGCAGGTTGTCCA
ACAGCATACCGACGCTGATCATCATCTGCAGGCCTGGCCATTTTCGCGTTTTTCTTCCCTCTCCCACCTT
TCCTCCCCCCCTCTTTTCCTTTCTTTTTCGTGCTACTTTGCGGCCTGCCCCGGCTGCCCGCCCCCCTTGC
CCCTCCTCTCCGTCTTTTTTCCGGTTCCCTCTTGTCTTTCTCTCTTCCTCCCTCCTCTTCCCCCCCCTTG
CCCTCTCTCCTCTCCCCCCCCCCGCTCCCCCCCCCTTCCCCCCCCCTTTTCTCTTTCTTCTTCCTCCCTG
CCCTCCCCGCCCTTCTCTCTTCCCGTCCCCTTCTATTTGTTTCTCTTTTCCTGTCCTTTCTTTATCTTAA
TTTTTCCCTACCCCCTTTCCCTTTTCTTTTTATTTTCCTCTTCTCCTCCCTTTTCTCCCCCTACTTTCTT
TTTCTTTCTATTCCTCTCATCACTCTTTCTTTATTTTTTTTTTCCCATCCCTCCCCCTTCATCTCTGTTC
ACCATATATCTTTTGTATTACTTCTTTCTCCCTTCTCCTTCCTCCTCCCCCTTTTTACTTCTTTTCCTTT
TTTCTTTTTACTTTCCCTCTCATCTCTATCTCTACNATCACTAATTTCTCCTTTTTTTCCCCTTCTTCTT
TTCCCTCCTCTTTCCCACCTCCTTTCCCCTTTCTCCCATTGCTATTCTCTTTTTTCCCCTTTTTTTCCCT
CTTTTCTCTCCATTTGCTTCCCTTCCATCGCCCTTTTCTCN
Blast against PA14
Processed Seqquence ID: 20260
5' Trim Position: 22 3' Trim Position: 253
Processed Sequence:
(Transposon Sequence in Red)		 
TATAGGGTGTCTAGAGACCGGAGACTTATCAGCCAACCTGTTAGTCGGTATTCAGGCGCGGGCCGCTGGA
GCAGCCGGCCAGCAGCGACAGGGTGAAAGCCAGTAGAACGAACGAACGCATGATGACTCCGAAAAAAATG
GGCAGCGCGGTCAGGCGGCGATGTGCAACACGCTGCGCAGGTTGTCCAACAGCATACCGACGCTGATCAT
CATCTGCAGGCCTGGCCATTT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
91672 91672 91673 91673 91674 91674
PA14 BLAST Hit ID: 91672       Show Transposon Map
Identities: 167/178 (93.82%) BLAST Bit Score: 280.0 Percent of Processed Sequence That Aligns: 76.62%
Query Start: 42 Query End: 219 Subject Start: 6450127 Subject End: 6450304 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6450127 PA14_72400 -
Raw Sequence Start: 6450064 PA14_72400 -
Extrapolated Insert Location: 6450128 PA14_72400 -
Default Offset Location: 6450127 PA14_72400 -
PAO1 BLAST Hit ID: 91673       Show Transposon Map
Identities: 166/178 (93.25%) BLAST Bit Score: 272.0 Percent of Processed Sequence That Aligns: 76.62%
Query Start: 42 Query End: 219 Subject Start: 6177215 Subject End: 6177392 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6177215 PA5485 -
Raw Sequence Start: 6177152 PA5485 -
Extrapolated Insert Location: 6177216 PA5485 -
Default Offset Location: 6177215 PA5485 -
PAO1 BLAST Hit ID: 91674       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 8.23%
Query Start: 68 Query End: 87 Subject Start: 612942 Subject End: 612923 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 612942 PA0554 -
Raw Sequence Start: 613031 PA0554 -
Extrapolated Insert Location: 612967 PA0554 -
Default Offset Location: 612968 PA0554 -
H PA14 Blast Hit H PAO1 Blast Hit