Mutant Report for Mutant ID: 42141
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 25902
Chromat Path: H:\Pa14\MrT7\439v1\439v1_H09_H9_080.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACATCAG
CCGCGGTGTCTACGTGATCACCGACGAGCGCCAGCAACGCCTGGTCAAGGTGCGCCTGGAGGACGATACC
CCCTCAAAACATCATCTGCAGGCCTGGCCATACCCNCTGNTTTCNTTCTGTGTTGNNTTGGGGGTGGTGT
AGAGACAACATAACGAAGCTTAGTGAAACAGTAAAAAAAAAGAGAGGGAGGCGATGGGAAATAAGCAGAA
GCTTCAGTCAAGTAATGCTGATACCCAAAGAAGGGAGGATCAAAATAGGTGGTGTGGAAGAAAAAAATAA
AAAAAGGAAGTGAAAGAATAAAAAAGGGGGAAGAACAAAATAAATAAAAAAAGGAGGGGGAAGAAGGAGA
GGCAAGAGGGTGCGGCCAGCAGGAAAAGGAAGAAAAAAAGAGGGGAACATAAAAGAGGAAGAGGAGAAAA
AAAGAGAGGAGAAAAAAAAAAAGAAGGAGGGAGTGTGTCAAGAGGACAACAAACATTTGATGAAAGGGAA
AAGAGTGGGGGGTTTTCAAAAGAGGGGGAAATCGGGGAGGGAAAAAAAAAAAGAAAAGAAAATATTGAAA
TGAAATAAATGTGGGAAGGAGTAATGGATAATAATAGAAGAGGGGGGAGGGGGAAAAGTAGAGGAGATGA
AAAAGAAGGAGAAAAATAAAAGGGGTGGGGAGAAAGAGAAAAGAAAAAAATAGAGAAAGTATGGAAAAAA
GGAAAAGAAAAAAAAAAAAGAGAAAAAAAGAAAAAAGGGAAGAAGGAGGAAAAAAAAAGAGATAAGAGAG
AAATATGGGATGAATGGTGGTGCGGCAGAAAAAACGACGTGGAACAGTAAAGAAGAAAAATGGTGGAGGG
TGTG
Blast against PA14
Processed Seqquence ID: 19779
5' Trim Position: 3 3' Trim Position: 175
Processed Sequence:
(Transposon Sequence in Red)		 
CAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACATCAGCCG
CGGTGTCTACGTGATCACCGACGAGCGCCAGCAACGCCTGGTCAAGGTGCGCCTGGAGGACGATACCCCC
TCAAAACATCATCTGCAGGCCTGGCCATACCC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
89811 89811 89812 89812
PA14 BLAST Hit ID: 89811       Show Transposon Map
Identities: 77/77 (100.00%) BLAST Bit Score: 153.0 Percent of Processed Sequence That Aligns: 44.19%
Query Start: 60 Query End: 136 Subject Start: 382026 Subject End: 382102 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 382026 PA14_04270,PA14_04280* +,+
Raw Sequence Start: 381964 PA14_04270,PA14_04280* +,+
Extrapolated Insert Location: 382027 PA14_04270,PA14_04280* +,+
Default Offset Location: 382027 PA14_04270,PA14_04280* +,+
PAO1 BLAST Hit ID: 89812       Show Transposon Map
Identities: 73/74 (98.64%) BLAST Bit Score: 139.0 Percent of Processed Sequence That Aligns: 42.44%
Query Start: 60 Query End: 133 Subject Start: 367856 Subject End: 367929 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 367856 PA0327 +
Raw Sequence Start: 367794 PA0327 +
Extrapolated Insert Location: 367857 PA0327 +
Default Offset Location: 367857 PA0327 +
H PA14 Blast Hit H PAO1 Blast Hit