Mutant Report for Mutant ID: 40962
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 24723
Chromat Path: H:\Pa14\MrT7\427v1\427v1_F06_F6_059.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATGGAGGT
CTCGGCGTGATCATCGATCTTTCCCAGTTGCCGGAGCCAGAGGTTATCGAAAACCTCGATTTCGAGACGA
TTTACCAAGAGCTGTTGGGCGACTTCCGCGAAGCCATGGCTGGCGAATGGACAGCGGAGGTGGAGTCGGA
TACGGTTCTCAAGCATCATCTGCAGGCCTGGCCACGAGAACTGCTGCTGCGGGCGCGGATCAACGATGCG
GCGCGGGCGGTGATGCTGGCATACGCCAGCGGTGCCGATCTCGACCAGATCGGTGCCGGCTTCAATGTGC
AGCGTTTGCTGATCAGGCCCGCTCAGCCCGAGGCGGTACCGCCGGTGGAGGCGCAGTACGAGAGCGACAA
GTCGCTGCGCAATCGCATCCAGCTCGCGTTCGAGCAGCTATCCGTCGCAGGACCGCGGAACGCCTATATA
GCCAATGCGCTGGGCGCGGATGGAACGGTGGCGGATGCCTCTGCGACCAGTCCGGCGCCCTGCGAAGTGC
TGATCAACGTGCTCGGGGGTGGAAGGCAACGGGCAGGCCCCGGAATGCGGTGTTTGCAGCAGTAGCGCCT
GGCTGCTGAACGCGGTAGGACGTTGCGTCCTGTCCGCGGATCCGGGTAACCGTTCCCCTTCGGCAAGAAT
CCTTCCCCTATCAGGGTCAAGGCTCTTCTTCTAACTGGTTTCCCGGCCCCGAAGGCCTAGCTGAATCGTT
GTCCCCGACCTAAGTTTGATTTGCGCTACATACATTTCCTCTCTACGTCCTCCTTAGGGCTCTAACATTC
CGTCATTTGGTACACGGTATACCACCCTAGATTGTGGTAGTGGGGTCTTTGTCATCTAAATTGTAATAGA
TCTTGCTTGGTAAGTGAN
Blast against PA14
Processed Seqquence ID: 18714
5' Trim Position: 2 3' Trim Position: 485
Processed Sequence:
(Transposon Sequence in Red)		 
TCGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATGGAGGTCT
CGGCGTGATCATCGATCTTTCCCAGTTGCCGGAGCCAGAGGTTATCGAAAACCTCGATTTCGAGACGATT
TACCAAGAGCTGTTGGGCGACTTCCGCGAAGCCATGGCTGGCGAATGGACAGCGGAGGTGGAGTCGGATA
CGGTTCTCAAGCATCATCTGCAGGCCTGGCCACGAGAACTGCTGCTGCGGGCGCGGATCAACGATGCGGC
GCGGGCGGTGATGCTGGCATACGCCAGCGGTGCCGATCTCGACCAGATCGGTGCCGGCTTCAATGTGCAG
CGTTTGCTGATCAGGCCCGCTCAGCCCGAGGCGGTACCGCCGGTGGAGGCGCAGTACGAGAGCGACAAGT
CGCTGCGCAATCGCATCCAGCTCGCGTTCGAGCAGCTATCCGTCGCAGGACCGCGGAACGCCT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
85016 85016 85017 85017 85018 85018 85019 85019
PA14 BLAST Hit ID: 85016       Show Transposon Map
Identities: 241/241 (100.00%) BLAST Bit Score: 478.0 Percent of Processed Sequence That Aligns: 49.69%
Query Start: 243 Query End: 483 Subject Start: 690264 Subject End: 690504 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 690264 PA14_08030 +
Raw Sequence Start: 690020 PA14_08020 +
Extrapolated Insert Location: 690082 PA14_08020 +
Default Offset Location: 690083 PA14_08020 +
PA14 BLAST Hit ID: 85017       Show Transposon Map
Identities: 162/163 (99.38%) BLAST Bit Score: 315.0 Percent of Processed Sequence That Aligns: 33.54%
Query Start: 60 Query End: 222 Subject Start: 690081 Subject End: 690243 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 690081 PA14_08020 +
Raw Sequence Start: 690020 PA14_08020 +
Extrapolated Insert Location: 690082 PA14_08020 +
Default Offset Location: 690083 PA14_08020 +
PAO1 BLAST Hit ID: 85018       Show Transposon Map
Identities: 239/241 (99.17%) BLAST Bit Score: 462.0 Percent of Processed Sequence That Aligns: 49.69%
Query Start: 243 Query End: 483 Subject Start: 677574 Subject End: 677814 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 677574 PA0618 +
Raw Sequence Start: 677330 PA0617 +
Extrapolated Insert Location: 677392 PA0617 +
Default Offset Location: 677393 PA0617 +
PAO1 BLAST Hit ID: 85019       Show Transposon Map
Identities: 161/163 (98.77%) BLAST Bit Score: 307.0 Percent of Processed Sequence That Aligns: 33.54%
Query Start: 60 Query End: 222 Subject Start: 677391 Subject End: 677553 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 677391 PA0617 +
Raw Sequence Start: 677330 PA0617 +
Extrapolated Insert Location: 677392 PA0617 +
Default Offset Location: 677393 PA0617 +
H PA14 Blast Hit H PAO1 Blast Hit