Mutant Report for Mutant ID: 39915
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 23676
Chromat Path: H:\Pa14\MrT7\416v1\416v1_G03_G3_023.ab1 Last Transposon Base: 61 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTGTATACGACTACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACTTGATAC
TGCCCCGCGCATCATCTGCAGGCCTGGCCAGACCTCGACGCTGAGATAGGGCGTGTCCAGCTGCTGGCGG
ATACTGCGGACAAACATCATCTGCAGGCCTGGCCAAANAAGGAAGAAAAAAAAAGGGACTGGAAAACAGA
GAAAAATATAAATAAGAACTAGAACAAATAAATAAAAAAATCCCGAAAAAAAAGAAGTTAAAAGAAAAAA
GAACAAGACAAAAAAAAAACAGAAAAAAAATAGAAAGCAATGAAAATATAAATAAAAAAAAAACAAATAG
GAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGAAAAAAAAAAACAAAAATAAAAAAAAACG
AAAGAAAAGAGAAAAAAAGAACAAAAAAAAAAAAAAAAGAAGAAAAAACAGGGAAAAAACCAATGCAAGA
ATCCAAGCGAACTGACAGAACCTAAACTATCAGTAACCAAATAAAAATAAGAATAAAAACCAGTAAAAAA
AATAATAAAAAATAAAAAGTAAAAAAAAAATAAAAATAATAAAAAAAAGAGAAGAAATAAATAAATAAGA
ACAAAAAAATATAAAACTTATATTAAATAGAAAAAAATAAAATAATGTAAACAAAAGAGAAAAAGAAATA
AAAGAAGTAAAATAAAATATATACAGTGTAAGAATAAAAAATATTAAATGCATAAACTAACAAAATAAAA
AATACGACTAAAAAAAAATACAAGCAGAAGAGACAAAGAACATAAAAAAAACAACCTGGGTTAACGAAAA
TACACCCAAAGCAAATAAACTATAATGTGAATCGATTCAAGATAAAATAAATATATACATAAACAATAAC
AATAACAACCAAGATATATAATATAATTACATATAATTAATAAGATAAGAAAATAATAAACATAGAA
Blast against PA14
Processed Seqquence ID: 17798
5' Trim Position: 11 3' Trim Position: 175
Processed Sequence:
(Transposon Sequence in Red)		 
TACGACTACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACTTGATACTGCCCCGCGCA
TCATCTGCAGGCCTGGCCAGACCTCGACGCTGAGATAGGGCGTGTCCAGCTGCTGGCGGATACTGCGGAC
AAACATCATCTGCAGGCCTGGCCA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
80793 80793 80794 80794
PA14 BLAST Hit ID: 80793       Show Transposon Map
Identities: 49/49 (100.00%) BLAST Bit Score: 97.6 Percent of Processed Sequence That Aligns: 29.27%
Query Start: 90 Query End: 138 Subject Start: 441713 Subject End: 441761 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 441713 PA14_05020 -
Raw Sequence Start: 441613 PA14_05020 -
Extrapolated Insert Location: 441674 PA14_05020 -
Default Offset Location: 441676 PA14_05020 -
PAO1 BLAST Hit ID: 80794       Show Transposon Map
Identities: 46/49 (93.87%) BLAST Bit Score: 73.8 Percent of Processed Sequence That Aligns: 29.27%
Query Start: 90 Query End: 138 Subject Start: 426977 Subject End: 427025 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 426977 PA0384 -
Raw Sequence Start: 426877 PA0384 -
Extrapolated Insert Location: 426938 PA0384 -
Default Offset Location: 426940 PA0384 -
H PA14 Blast Hit H PAO1 Blast Hit