Mutant Report for Mutant ID: 36301
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 20062
Chromat Path: H:\Pa14\MrT7\379v1\379v1_B01_B1_009.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATCAATA
CGCTGCAAGGTCTTGTTCTGTCTGTCTGGCCACCCGACGGCGAGCGTGTCCTGAGCGTTTCGATAGGACT
CACTTCCGAAGCGGCCTACGAGTTCCTCAAGGCGATCTTTCTCTGCTCGTTCGGCCTGATGATGAGCGAA
GCCAGGAAACTGGACGAAGAAGTAAGAGCCTTCATATGACGACCCCATGATCATCATCCGACTAGACGTC
ATCCTGGCGAAGAACAAGGTCCGCTCCAAGGAGCTGGCGGAAGCCATCGGTATCACCGAACAGAACCTGT
CCCTGCTGAAGAACGGCAAGGTGAAGGGCATCCGCCTCGACACCCTGGAGAAGATCTGCGCCTTCCTGGA
CTGCCAGCCGGGCGACATCCTGGAATACGTCGCGGGAGCATCATTCTGCAGGCCTGGCCATTTTGTATGT
TTTGCATAAACTGAATCGCTTCTCATTTTTGAATATTTCTATCTCTCGCGACAGATTTTAATAAATAATT
TACCCCATTTCTCTACCTTCCACATCTAGACGCTGTTTTTAATATAACTTAACTAAGAACAATACAACTA
ATAATATAAGAAAGGTACAAAATCTCATAATTTTGCAAATACAGTGACAACAGACTTTAAAAGCACTATA
TACGAAATTTTTTTCACCATACTTATTTCATACGAATGGTTCTCTTAAGAACGACTGTTGATGAGAACAC
TACACGAACTTCTACTTTAGACTCAATCACCTGCTACATAAGAAATCCTAATAGCCTCCTGATACTTCTT
CGTATCTCTATATTTATGTTACATGTGATAATCTATCTCACCACTATTGATACTTTCACTCATATATATT
CTTCACTACATTGTCTCTCTATTATAG
Blast against PA14
Processed Seqquence ID: 14779
5' Trim Position: 2 3' Trim Position: 444
Processed Sequence:
(Transposon Sequence in Red)		 
TCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATCAATACG
CTGCAAGGTCTTGTTCTGTCTGTCTGGCCACCCGACGGCGAGCGTGTCCTGAGCGTTTCGATAGGACTCA
CTTCCGAAGCGGCCTACGAGTTCCTCAAGGCGATCTTTCTCTGCTCGTTCGGCCTGATGATGAGCGAAGC
CAGGAAACTGGACGAAGAAGTAAGAGCCTTCATATGACGACCCCATGATCATCATCCGACTAGACGTCAT
CCTGGCGAAGAACAAGGTCCGCTCCAAGGAGCTGGCGGAAGCCATCGGTATCACCGAACAGAACCTGTCC
CTGCTGAAGAACGGCAAGGTGAAGGGCATCCGCCTCGACACCCTGGAGAAGATCTGCGCCTTCCTGGACT
GCCAGCCGGGCGACATCCTGGA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
66985 66985 66986 66986 66987 66987 66988 66988
PA14 BLAST Hit ID: 66985       Show Transposon Map
Identities: 374/383 (97.65%) BLAST Bit Score: 688.0 Percent of Processed Sequence That Aligns: 86.43%
Query Start: 60 Query End: 442 Subject Start: 6355587 Subject End: 6355969 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6355587 PA14_71320 +
Raw Sequence Start: 6355526 PA14_71320 +
Extrapolated Insert Location: 6355589 PA14_71320 +
Default Offset Location: 6355589 PA14_71320 +
PA14 BLAST Hit ID: 66986       Show Transposon Map
Identities: 110/128 (85.93%) BLAST Bit Score: 111.0 Percent of Processed Sequence That Aligns: 28.73%
Query Start: 303 Query End: 430 Subject Start: 2431927 Subject End: 2431800 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2431927 PA14_28080 +
Raw Sequence Start: 2432231 PA14_28090 +
Extrapolated Insert Location: 2432168 PA14_28090 +
Default Offset Location: 2432168 PA14_28090 +
PAO1 BLAST Hit ID: 66987       Show Transposon Map
Identities: 372/383 (97.12%) BLAST Bit Score: 672.0 Percent of Processed Sequence That Aligns: 86.43%
Query Start: 60 Query End: 442 Subject Start: 6082671 Subject End: 6083053 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6082671 PA5402 +
Raw Sequence Start: 6082610 PA5402 +
Extrapolated Insert Location: 6082673 PA5402 +
Default Offset Location: 6082673 PA5402 +
PAO1 BLAST Hit ID: 66988       Show Transposon Map
Identities: 109/128 (85.15%) BLAST Bit Score: 103.0 Percent of Processed Sequence That Aligns: 28.73%
Query Start: 303 Query End: 430 Subject Start: 3142335 Subject End: 3142462 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3142335 PA2785 +
Raw Sequence Start: 3142031 PA2784 +
Extrapolated Insert Location: 3142094 PA2784 +
Default Offset Location: 3142094 PA2784 +
H PA14 Blast Hit H PAO1 Blast Hit