Mutant Report for Mutant ID: 36016
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 19777
Chromat Path: H:\Pa14\MrT7\376v1\376v1_B04_B4_031.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAACGGCG
ACGCGCCTCGAGACCTTCCTCCAGGCTGACGGCGACGAAACGCGCCTTCTGGTTCGGTTGCATCTGCGCC
AGCCGGTTCAGGTCGGGGCTGATCACCGTGCCCAGCGTGGCATATCCCCCACCGGCATCATCTGCAGGCC
TGGCCAAAATAANTTNNAGNNCNGCCNGGTACCTGGATCGAGTCGATGGTATAGCAAGACGTCGACTATG
TTGGACGGTTTCCGAGCCTGCGCCTAATGGCTGTTCGCGCCGGCTCGCACCGGAGCGCCGTGCCGCCCTT
GAAGTCGGTTTCTTTTGCTTTCCGCCTTCGAGCCTAACGGTCCGGGTCTCGGCTGATAACACCTCTACCC
GCGTCGGGGGTCTAGGCGCACGGACGTCATGGCCGGCTAATCAACCCTTAGCCTCCCTTCTGCTCCGTCT
TTCTCTTTCTATCTAGGGGGGAAACGACTAAACCCTCTCAGGCTATAAATGATATGGTTGCTCCTCATTG
GCAATACTATTCTCCCCTCCTCCATTAGCCGATTTTTTAGGCAATTTACTGCCTCCTATCCATCATCTCT
TAGCTTTGCTCATTTCCCCTGATTCCTTGTATGCCCCCCTTCCAACCGCTCATACACCATCCGTCCGCCG
TGTAGTTTAGAATATGTTTACATATTGTCTCTGACTGCTGCACAATCCAGAGTAGATCTATTGTCCACCA
GCCTCTACCGGAACTCCCCCTCACTCTTGTCCATCCACTTCTCTTATTTGTCGTCTCTTCCTCTTGTACA
TGCTGTGTTTCGTCTTACATTCTTTTTTGGATCAGAATCCACTGCCGCACTCATCAATTCGAGTGCATTT
GTCTTGTAGCTGAATCT
Blast against PA14
Processed Seqquence ID: 14500
5' Trim Position: 3 3' Trim Position: 212
Processed Sequence:
(Transposon Sequence in Red)		 
CAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAACGGCGACG
CGCCTCGAGACCTTCCTCCAGGCTGACGGCGACGAAACGCGCCTTCTGGTTCGGTTGCATCTGCGCCAGC
CGGTTCAGGTCGGGGCTGATCACCGTGCCCAGCGTGGCATATCCCCCACCGGCATCATCTGCAGGCCTG
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
65137 65137 65138 65138
PA14 BLAST Hit ID: 65137       Show Transposon Map
Identities: 133/133 (100.00%) BLAST Bit Score: 264.0 Percent of Processed Sequence That Aligns: 63.16%
Query Start: 60 Query End: 192 Subject Start: 568973 Subject End: 568841 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 568973 PA14_06470*,PA14_06480 +,-
Raw Sequence Start: 569035
Extrapolated Insert Location: 568972 PA14_06470*,PA14_06480 +,-
Default Offset Location: 568972 PA14_06470*,PA14_06480 +,-
PAO1 BLAST Hit ID: 65138       Show Transposon Map
Identities: 132/133 (99.24%) BLAST Bit Score: 256.0 Percent of Processed Sequence That Aligns: 63.16%
Query Start: 60 Query End: 192 Subject Start: 556185 Subject End: 556053 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 556185 PA0496 -
Raw Sequence Start: 556247
Extrapolated Insert Location: 556184 PA0496 -
Default Offset Location: 556184 PA0496 -
H PA14 Blast Hit H PAO1 Blast Hit