Mutant Report for Mutant ID: 33062
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 16838
Chromat Path: H:\Pa14\MrT7\345v1\345v1_D02_D2_016.ab1 Last Transposon Base: 113 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
NNNNTTTTNNNNCCAAATTATAGATAAAATATGAAAAATAAAACCNCNCTGTTCAGTTTGTATACGACTC
ACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACTCAATATCGCCTCGGTGGCCGCCTT
CCAGCCCGGCGCCTGGATGAGCAACTACTACGCCAGCAAGGCCTACGTCCTGCACTTCTCGGAAGGCTTG
CGGGAAGAACTGAAAGGGCGCGGCGTCAAGGTCTCGGTGCTGTGCCCTGGGCCAACCCGTACGGCATTCT
TCCGGGAAGCCGGTATGCGGCTCGGCAAGTTCAACCGGAGCAAGCTGATGATGACCCAGAAGGAAAAAAA
ACTGAAAGGCCCTGCAACGAAAGGACAAGAACCGTTACCTTTATTTGAACTAGGTTGATCATATATACTG
CTAGCCGCTGAGTCCATATATGGCACTAACGCTAATATGGAGCCAACGACTCGAACACGAGAATCAGAGA
TTAACAGAAGTACCAAAACGATCAACAGATACATTCACTGTAGCTCACCTCAAAGGAAATGATAAAATCT
AGAACAACCCAAAATTGATAATATCAGGGGCAGCTTACCGCCCCCAAACCCCATAGCTGCTCCCATATTC
ACTACAGCACACCTCTCCTACATTTTTGTTATATCTAATTGACATCTATCTGTCCCATAACAATTCCTTA
TGCCATGTTTCCATAGCAGAACTCCCTATATTACAGATTATTCCGAATCATCTCTAGAATCCTACCCTAC
ACGTCTACCCCTTCTTTAGTTATGTCGTAACACACTAGTACACTACATAAACACCCTCTCACTACTATCT
ACACATCCAAATTAATATAAGTTAATCACACTCACTCTCGATAATCNTCACTATGTCTCACGCCTCACTA
TGAGTGCATAGAAATCCACAAGAAGACTTTACCAACACCACTATACATCACAGAATAATAATATCTCACG
ATAAGTGAAGAACATCTACATCTACCCGGTGAAACTTAATGAAGTACTATCACAATCATACATATCAAGC
TCAAACATGTGACTTCATATACAGACTACAGATAAATCTTGCTACGCGTCGTACCAGCTGTGCCTCAAGC
GCTACTATACTTATCTTGTATCGTAGCATTGGGAAGTGGTAACGATGAGCTTTAATATGATTCTGCTTTT
ATACTTCGCTGTCGCCTCCGCCGTAACAATTACGCAACAATCTACAAAACGCCGCAACCGCGTCTACATA
CCGC
Blast against PA14
Processed Seqquence ID: 11925
5' Trim Position: 60 3' Trim Position: 337
Processed Sequence:
(Transposon Sequence in Red)		 
TATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACTCAATATCGCCTCGG
TGGCCGCCTTCCAGCCCGGCGCCTGGATGAGCAACTACTACGCCAGCAAGGCCTACGTCCTGCACTTCTC
GGAAGGCTTGCGGGAAGAACTGAAAGGGCGCGGCGTCAAGGTCTCGGTGCTGTGCCCTGGGCCAACCCGT
ACGGCATTCTTCCGGGAAGCCGGTATGCGGCTCGGCAAGTTCAACCGGAGCAAGCTGATGATGACCC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
53845 53845 53846 53846
PA14 BLAST Hit ID: 53845       Show Transposon Map
Identities: 221/225 (98.22%) BLAST Bit Score: 414.0 Percent of Processed Sequence That Aligns: 80.87%
Query Start: 53 Query End: 277 Subject Start: 722604 Subject End: 722380 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 722604 PA14_08440 +
Raw Sequence Start: 722716 PA14_08440 +
Extrapolated Insert Location: 722603 PA14_08440 +
Default Offset Location: 722653 PA14_08440 +
PAO1 BLAST Hit ID: 53846       Show Transposon Map
Identities: 219/225 (97.33%) BLAST Bit Score: 398.0 Percent of Processed Sequence That Aligns: 80.87%
Query Start: 53 Query End: 277 Subject Start: 711620 Subject End: 711396 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 711620 PA0658 +
Raw Sequence Start: 711732 PA0658 +
Extrapolated Insert Location: 711619 PA0658 +
Default Offset Location: 711669 PA0658 +
H PA14 Blast Hit H PAO1 Blast Hit