Mutant Report for Mutant ID: 29239
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 13015
Chromat Path: H:\Pa14\MrT7\305v1\305v1_E07_E7_053.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGGCCCA
GCGGAAGCGCTGCTTCTTGAAGTCGATGAAGGTATCGGGCATCACCACCTTGCCGTAGCTGCGCTCGAAG
TAGGCGGCCGACAGGCCCTTCTCGAACACCCGCAGGCCCAGCTCGGCGTCTTCGGTGATGCACCATTCCG
GCCATACCAGCTCGCCCGGGAAGTTGCAGTGTTGGTTACCACTCGGTTGATAGCACTTCAATCTCATCGC
GGATTACATGTGGGACAACTCCGCTAAGATAGCAGCCTTTGAATTCGGCAGACGACAACCGCTTGAAACC
GCTAAGGGGTCTTTCTCTTCCGATTTTTGAGGAGACCCCAGAAAGGAACCCACCGGTACTAAGTACGTCT
TTTGGCGTACCTCTAACCGGCTGGGGGGGAGAGTACTGTTTAGGGGTGTGGCGGGCCCCCTTCCCTCGGG
GGCAGGGAAACCCCGGGGACCATAGCCAAATCCTAGGGACCACCACCCTATGGAATTTTCCAAACGGGCC
ATAATGCCGGACACCCGTAATGTTATTTCTCCGGCCTTTGGGAGCGAACCTTCTCCTGCCTGAAAGGGAA
ACCCTTCCAGCGCTGTCTGGGGTAAAATCCCTCATGTTTTTATTCTCCACATTTACTGATTCCTTAGCGC
GTTTTTTGTGTTTCTTACCCCTCCCGGAGGTTACGTTGTCCCGAAGCTTCGGGGCTTCTCTAGGGCCATA
TTTCTGGCCCCCTTTTTATTCTTCTCCCTGCTCCCCTGTCTCTTTACGGGTTTCTTGTTCTTCCCGGCCT
TGTCTTCCGCGTTTTAATCACCCCTAAGTTCTTTTGTTTAATCCTCTTGTCCTTATCGTTTTTGGT
Blast against PA14
Processed Seqquence ID: 8794
5' Trim Position: 4 3' Trim Position: 219
Processed Sequence:
(Transposon Sequence in Red)		 
AGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGGCCCAGCGG
AAGCGCTGCTTCTTGAAGTCGATGAAGGTATCGGGCATCACCACCTTGCCGTAGCTGCGCTCGAAGTAGG
CGGCCGACAGGCCCTTCTCGAACACCCGCAGGCCCAGCTCGGCGTCTTCGGTGATGCACCATTCCGGCCA
TACCA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
39586 39586 39587 39587
PA14 BLAST Hit ID: 39586       Show Transposon Map
Identities: 152/153 (99.34%) BLAST Bit Score: 295.0 Percent of Processed Sequence That Aligns: 70.70%
Query Start: 59 Query End: 211 Subject Start: 4387788 Subject End: 4387636 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4387788 PA14_49360,PA14_49370* -,+
Raw Sequence Start: 4387850 PA14_49360,PA14_49370* -,+
Extrapolated Insert Location: 4387787 PA14_49360,PA14_49370* -,+
Default Offset Location: 4387787 PA14_49360,PA14_49370* -,+
PAO1 BLAST Hit ID: 39587       Show Transposon Map
Identities: 150/153 (98.03%) BLAST Bit Score: 280.0 Percent of Processed Sequence That Aligns: 70.70%
Query Start: 59 Query End: 211 Subject Start: 1261224 Subject End: 1261376 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1261224 PA1163 -
Raw Sequence Start: 1261162 PA1163 -
Extrapolated Insert Location: 1261225 PA1163 -
Default Offset Location: 1261225 PA1163 -
H PA14 Blast Hit H PAO1 Blast Hit