Mutant Report for Mutant ID: 27444
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 11220
Chromat Path: H:\Pa14\MrT7\286v1\286v1_G12_G12_100.ab1 Last Transposon Base: Transposon Sequence
Match Quality:
Raw Sequence:
(Transposon Sequence in Red)		 
NNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGAAAANNNNGGGGGGNNNNANGAAANNGNNNNNGTTGT
TCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGAGACTTATCAGCCAACCTGTTACTAAGAAG
AACCGCACCGACCATTCATCTGGGGGGGTGGACAGAGCCGGCGCTCGAAGGACAGAAAATCGGAAGCGGG
GGAGAAATGCGCGCTCATCGCACCCACGAAACCCAGGCAGAGCGGGACGACCACCCAGAGAATAAGGAAG
GGCCCGCAGCATTCCTAGGAAAGCTGCACGACCTGGGCGGCACCCGAAACCTTGGAACGGCTTTATCGCC
CGTCCCGTAGAGGGGATGCCTGAACGCTCGCCTGAAAAGACAAAGCCCCCGGAACAACAGGCATGCGATG
AGCTTTTCTTCTATGTTATGGGGGAGAGACAGGGTTTTGAACCCAGTAAGCCATTGCTGCCTTAACGGTA
TTTAAAATTGTTCACGTTCTATCACTCGGGACAGTTTACAGACCGGGCCCAGAGATTTCTGTTGTGAACT
AAGATCGGAAAAACTTTCTCGAATAATTCTTATATGAGCTCTCGCGGGTTGCTGCGGTTATGTCGTAAGG
CTGGTTTCGCAGGTTTTTGTTTACATCTACCGTGGCTATCATTCTATGTGGCGAAGATCCTGCCGGAATA
GAGTCTATGCACGCCTTCTTTTTACTTTCTAGTAGTGAAGGAAGAAATCGTCGTTGTATATATTAGACGT
GTTTGTCGAGGTTTTTATGTGGAATTATATGCCTAATCAAACTATTTCTCCCTTTCTTAACATGTGTCTT
TCTTTATTTATGAATTAAGAATAAATGTATTGTTTTTCAAATATAGTAGTTAGTAATCACGCAGTAACAC
ATACATTAATTTAAGATTATTATGGCTGGTTACACCGTGAAGGCTCACCCCTTCGTTGTAACATGTATGT
GGTGCTGTTTTCGAGAATAAGAAGT
Blast against PA14
Processed Seqquence ID: 7190
5' Trim Position: 84 3' Trim Position: 307
Processed Sequence:
(Transposon Sequence in Red)		 
ACTCACTATAGGGGTCTAGAGACCGGAGACTTATCAGCCAACCTGTTACTAAGAAGAACCGCACCGACCA
TTCATCTGGGGGGGTGGACAGAGCCGGCGCTCGAAGGACAGAAAATCGGAAGCGGGGGAGAAATGCGCGC
TCATCGCACCCACGAAACCCAGGCAGAGCGGGACGACCACCCAGAGAATAAGGAAGGGCCCGCAGCATTC
CTAGGAAAGCTGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
32262 32262 32263 32263 32264 32264 32265 32265
PA14 BLAST Hit ID: 32262       Show Transposon Map
Identities: 127/130 (97.69%) BLAST Bit Score: 234.0 Percent of Processed Sequence That Aligns: 57.85%
Query Start: 94 Query End: 223 Subject Start: 4552714 Subject End: 4552843 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4552714
Raw Sequence Start: 4552537 PA14_51220 -
Extrapolated Insert Location:
Default Offset Location: 4552600 PA14_51220 -
PA14 BLAST Hit ID: 32263       Show Transposon Map
Identities: 25/25 (100.00%) BLAST Bit Score: 50.1 Percent of Processed Sequence That Aligns: 10.76%
Query Start: 47 Query End: 71 Subject Start: 4552667 Subject End: 4552691 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4552667
Raw Sequence Start: 4552537 PA14_51220 -
Extrapolated Insert Location:
Default Offset Location: 4552600 PA14_51220 -
PAO1 BLAST Hit ID: 32264       Show Transposon Map
Identities: 127/130 (97.69%) BLAST Bit Score: 234.0 Percent of Processed Sequence That Aligns: 57.85%
Query Start: 94 Query End: 223 Subject Start: 1097217 Subject End: 1097088 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1097217
Raw Sequence Start: 1097394 PA1014 -
Extrapolated Insert Location:
Default Offset Location: 1097331 PA1014 -
PAO1 BLAST Hit ID: 32265       Show Transposon Map
Identities: 25/25 (100.00%) BLAST Bit Score: 50.1 Percent of Processed Sequence That Aligns: 10.76%
Query Start: 47 Query End: 71 Subject Start: 1097264 Subject End: 1097240 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1097264
Raw Sequence Start: 1097394 PA1014 -
Extrapolated Insert Location:
Default Offset Location: 1097331 PA1014 -
H PA14 Blast Hit H PAO1 Blast Hit