Mutant Report for Mutant ID: 26586
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 10362
Chromat Path: H:\Pa14\MrT7\277v1\277v1_H06_H6_060.ab1 Last Transposon Base: Transposon Sequence
Match Quality:
Raw Sequence:
(Transposon Sequence in Red)		 
TTATCTGCCAACCTGTTAATGCGCTGGCGACTGCCGCCAGATCGTCGAATATCAGGGTGCCCTCTGGCAA
GGGCTTGCCCAGCGTACGTACACCTTTTCCGGTCTTTACCAACACCGGCTGACAATCGACGGCCCGCGCC
GCCTCCAGGTCACCGATGCTGTCGCCGACGAACCAGATACCCGACAGATCGATCCTGTGGTGGTTGGCGA
ACTGCCGCAGCATACCCGGCTTCTGCATCGTCTGGTCGCCAGCCCATTCTGGCTTTTGCTGTATATTTAC
GATGAGGAAAACCTTAGCGCCATGCTTCGTGAATAGTTGGAGTGAGTGTAGCATGGTTGGTCTGACGCTC
TGCCTCGCCACAAAGATACACTGACAAATGATCAGACTTAAATGTTGACTCCCCTCTCGTGTTCTCTCTT
TATGATACTACTTTATTTCCTATTCCCTCTCTCTCTATATGTGTTTTTGATTTGAACTTGTAATGTAGTA
TCTATTATGTAAACTCACACATCTTCTGGGATGTATTGTTTTCTATTGACGCTGTCAATTATGCCTTCAT
TCACTCGCATCCATACCTACGTACACATAATATGTATTAATTGTAATGAGTAAGAATTATATATATATGT
TGTGAAGTAAATGTTCTATTTTATGTCTTTTTATTTGTTACTATATCTTCTATGCTACATAATATAGATA
TAAATTATTCATATCCTCTTATTCTATCAGATAGACTAACATAGAATTACTTTATACTCTGACGCTAATA
AAATGATATGCACCGCTTTAATGTGATCCTAGTTTATGATTTTTGATACATTTTGCTTGTGTTTTGTTTC
TTTAGTTTCTTCCTATTCAATTATCATTATAATATTAGTTTTATGTACTATGATCCGTTGTACCGACACG
TGGGTCATCTCTTCCCTGAGCCATTAATCCGGTATTGCTGGCTACGTCTGTCCCATTTCTCTGCTTCATA
CACTTTTCTTCCCGTG
Blast against PA14
Processed Seqquence ID: 6398
5' Trim Position: 2 3' Trim Position: 233
Processed Sequence:
(Transposon Sequence in Red)		 
ATCTGCCAACCTGTTAATGCGCTGGCGACTGCCGCCAGATCGTCGAATATCAGGGTGCCCTCTGGCAAGG
GCTTGCCCAGCGTACGTACACCTTTTCCGGTCTTTACCAACACCGGCTGACAATCGACGGCCCGCGCCGC
CTCCAGGTCACCGATGCTGTCGCCGACGAACCAGATACCCGACAGATCGATCCTGTGGTGGTTGGCGAAC
TGCCGCAGCATACCCGGCTTC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
28967 28967 28968 28968 28969 28969
PA14 BLAST Hit ID: 28967       Show Transposon Map
Identities: 211/217 (97.23%) BLAST Bit Score: 383.0 Percent of Processed Sequence That Aligns: 93.51%
Query Start: 15 Query End: 231 Subject Start: 7812 Subject End: 8028 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 7812 PA14_00070 -
Raw Sequence Start: 7796
Extrapolated Insert Location:
Default Offset Location: 7859 PA14_00070 -
PAO1 BLAST Hit ID: 28968       Show Transposon Map
Identities: 209/217 (96.31%) BLAST Bit Score: 367.0 Percent of Processed Sequence That Aligns: 93.51%
Query Start: 15 Query End: 231 Subject Start: 7812 Subject End: 8028 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 7812 PA0006 -
Raw Sequence Start: 7796
Extrapolated Insert Location:
Default Offset Location: 7859 PA0006 -
PAO1 BLAST Hit ID: 28969       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 8.66%
Query Start: 133 Query End: 153 Subject Start: 1268119 Subject End: 1268099 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1268119 PA1169 -
Raw Sequence Start: 1268253 PA1169 -
Extrapolated Insert Location:
Default Offset Location: 1268190 PA1169 -
H PA14 Blast Hit H PAO1 Blast Hit