Mutant Report for Mutant ID: 23737
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 7513
Chromat Path: H:\Pa14\MrT7\248v1\248v1_C01_a_005.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 2
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCAGTTTGTATACGACTCCTATAGGGNTCTAGAGACCGGGNACTTATCAGCCTACCTGTTATCCATGT
CGCCAGAGGTGAANAGTACGTCCTTGGCCCGGAATACCTCTCCCCCCCATCATCTGCAGGCCTGGCCAGN
NTTGATGNTGCCGACCGCGGCTACCTGGTCTCCCTGTTCCAGGATGCCTGTGCCACCCATACCCCTTCCC
CACATCTTCTGCATGCCTGGCCACATATTTCTTTTTATTCCTTGTCTTTCTTCTCTTTTGTGTTTTTATC
CTTCGGCACCGCGTAATTTCCTTTTCTTCCTCCGTCAACACCTATATTGTATTTTGGTATCCTTTTAACA
TGTTTGCCGTTTTCTTTTTACTACTCTCGTTTTTCTATTTTCTTATCCCATTTCCCTCTTGTTTTCCTTA
ATCTCCTATCTTTCTTTGTACTTCTTGTCCTTTTACCTTGCTTTTCTCTTTTCCTCTAAAGTTTCTTCCT
CCCCTTCTGCGGCCCCGTCGGCTCCATTTGTCTGTCCAAATAAGCTTCGTGTACTATTTTCTATTTTGTT
ACTGTTCTCTCGTCCCTCTTTTTTCATTCCGTTTGTCTGTCTTTTTTTTTACATTTCTACAATTTTTATC
TTTTTTTCTTCTTTGAACTTTCGTTATTTGTTGTCCCCCCTTTTTTCTTCCACCTCTTTTTCCAACGTAA
GTTAACCCTTCTTATGTGTTTCGTATTAATACCAAAGTGTACTTAATGCAACATCTCGCTCCTCTCACCC
TCCTGCTTTGCCCCTTCCAATACTTATATTAATTAGAACCTGTGCGTCAGTGCGGTGATATAGATCAAAA
GGAATTAGCGTTCATCTTACCGCGCTCTCACGCTAACACCTTCACTGGTCAGTAGATAGACCACTAAAAC
G
Blast against PA14
Processed Seqquence ID: 3870
5' Trim Position: 56 3' Trim Position: 210
Processed Sequence:
(Transposon Sequence in Red)		 
CCTGTTATCCATGTCGCCAGAGGTGAANAGTACGTCCTTGGCCCGGAATACCTCTCCCCCCCATCATCTG
CAGGCCTGGCCAGNNTTGATGNTGCCGACCGCGGCTACCTGGTCTCCCTGTTCCAGGATGCCTGTGCCAC
CCATACCCCTTCCC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
16371 16371 16372 16372 16373 16373 16374 16374
PA14 BLAST Hit ID: 16371       Show Transposon Map
Identities: 53/56 (94.64%) BLAST Bit Score: 87.7 Percent of Processed Sequence That Aligns: 35.71%
Query Start: 94 Query End: 149 Subject Start: 6472647 Subject End: 6472702 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6472647 PA14_72660 +
Raw Sequence Start: 6472498 PA14_72660 +
Extrapolated Insert Location: 6472560 PA14_72660 +
Default Offset Location: 6472561 PA14_72660 +
PA14 BLAST Hit ID: 16372       Show Transposon Map
Identities: 46/50 (92.00%) BLAST Bit Score: 69.9 Percent of Processed Sequence That Aligns: 31.82%
Query Start: 6 Query End: 55 Subject Start: 65748 Subject End: 65699 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 65748 PA14_00640 -
Raw Sequence Start: 65809 PA14_00640 -
Extrapolated Insert Location: 65747 PA14_00640 -
Default Offset Location: 65746 PA14_00640 -
PAO1 BLAST Hit ID: 16373       Show Transposon Map
Identities: 51/56 (91.07%) BLAST Bit Score: 71.9 Percent of Processed Sequence That Aligns: 35.71%
Query Start: 94 Query End: 149 Subject Start: 6199408 Subject End: 6199463 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6199408 PA5507 +
Raw Sequence Start: 6199259 PA5507 +
Extrapolated Insert Location: 6199321 PA5507 +
Default Offset Location: 6199322 PA5507 +
PAO1 BLAST Hit ID: 16374       Show Transposon Map
Identities: 46/50 (92.00%) BLAST Bit Score: 69.9 Percent of Processed Sequence That Aligns: 31.82%
Query Start: 6 Query End: 55 Subject Start: 67386 Subject End: 67337 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 67386 PA0051 -
Raw Sequence Start: 67447 PA0051 -
Extrapolated Insert Location: 67385 PA0051 -
Default Offset Location: 67384 PA0051 -
H PA14 Blast Hit H PAO1 Blast Hit