Mutant Report for Mutant ID: 56790
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 31335
Chromat Path: H:\Pa14\MrT7\592v1\592v1_E06_E6_040.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 3
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCGTTTAGAACGAATAGGTATAGTGCGTGGCTAGAGAGCGGAAACTTATCAGCCAACCTGTTACATGC
ACTACGGCGTGGTCCCGGACATCCTCTCCAGCGCCAAGAGCCTGGGCGGCGGCTTCCCTATCGGTGCGAT
GCTGACCACCGGCGAGATCGCCAAGCACCTGTCGGTCGGCACCCACGGCACCACCTACGGCGGCAACCCG
CTGGCCTCGGCGGTGGCCGAGGCGGCGCTGGACGTGATCAATACCCCGGAAGTGCATCATCTGCAGGCCT
GGCCAAAGAGNGACTAGGAAATTTCGAAAATTAAACATCCTGACCTTATTTTCTTCTCTTCCTTGTTTGT
AACTACCGTTGTAGATAAAGTTAAAGTCGTTCTGTGGGCTGACGCTGCGGACGGGTGTTGATCCATAAGA
TCGGAGGTGCTAGTGCAGATTCTAAAAGAATAACAAAATGGTTAATAATACCGTATGTGATAGTAAATAT
ATTATAAAAGATTGTGTTGTTAGTCATTTATGTGCAAATGTGTATTTATATAGCAAGAGTGAACCAGGAT
TGCGAGAGCCGCGACCAAATCGACTTTTCATTGCATATATCAACACTAAAAATAGGTAAGATACACGGAG
ATGCGTTTATTCTACGTCAATAGAGACATTTACCCACTGTGGCTGAGATCATCATCACGATAATTATTTA
CTTCGTCCTCTATTCGTGTTTTTGTCATCTCCATTTTTTTTCGTGTCTGTCCTTTAAACGTTCAATATGT
TATACTCCTTATATATTCATAATTAACTTATTCCAAATTGTTGGCTAGGGTCCCCTACAGGATCTGCTCA
GTACTATACTGAAACCGCATTTCCTTTGGCGTGTTGTCAGCCGAAGTGTTTTTATGCTAGATGTTATATT
TCAAGTTCTTTACTTGCTATTGTTATCTTTGACAATCACTGTTTCACACGTATCGTCTCTTGTTATTAGC
TAATTTGTATGATCACGATGGCACACTATCCGTCT
Blast against PA14
Processed Seqquence ID: 24085
5' Trim Position: 40 3' Trim Position: 289
Processed Sequence:
(Transposon Sequence in Red)		 
CGGAAACTTATCAGCCAACCTGTTACATGCACTACGGCGTGGTCCCGGACATCCTCTCCAGCGCCAAGAG
CCTGGGCGGCGGCTTCCCTATCGGTGCGATGCTGACCACCGGCGAGATCGCCAAGCACCTGTCGGTCGGC
ACCCACGGCACCACCTACGGCGGCAACCCGCTGGCCTCGGCGGTGGCCGAGGCGGCGCTGGACGTGATCA
ATACCCCGGAAGTGCATCATCTGCAGGCCTGGCCAAAGA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
105220 105220 105221 105221 105222 105222 105223 105223
PA14 BLAST Hit ID: 105220       Show Transposon Map
Identities: 202/202 (100.00%) BLAST Bit Score: 400.0 Percent of Processed Sequence That Aligns: 80.72%
Query Start: 24 Query End: 225 Subject Start: 4672961 Subject End: 4672760 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4672961 PA14_52720,PA14_52710* +,-
Raw Sequence Start: 4673024 PA14_52720,PA14_52710* +,-
Extrapolated Insert Location: 4672960 PA14_52720,PA14_52710* +,-
Default Offset Location: 4672961 PA14_52720,PA14_52710* +,-
PA14 BLAST Hit ID: 105221       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 9.24%
Query Start: 99 Query End: 122 Subject Start: 6125111 Subject End: 6125088 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 6125111 PA14_68700 +
Raw Sequence Start: 6125249 PA14_68700 +
Extrapolated Insert Location: 6125185 PA14_68700 +
Default Offset Location: 6125186 PA14_68700 +
PAO1 BLAST Hit ID: 105222       Show Transposon Map
Identities: 201/202 (99.50%) BLAST Bit Score: 392.0 Percent of Processed Sequence That Aligns: 80.72%
Query Start: 24 Query End: 225 Subject Start: 978630 Subject End: 978831 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 978630 PA0895 +
Raw Sequence Start: 978567 PA0895 +
Extrapolated Insert Location: 978631 PA0895 +
Default Offset Location: 978630 PA0895 +
PAO1 BLAST Hit ID: 105223       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 9.24%
Query Start: 99 Query End: 122 Subject Start: 5852900 Subject End: 5852877 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5852900 PA5200 +
Raw Sequence Start: 5853038 PA5200 +
Extrapolated Insert Location: 5852974 PA5200 +
Default Offset Location: 5852975 PA5200 +
H PA14 Blast Hit H PAO1 Blast Hit