Mutant Report for Mutant ID: 56681
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 31226
Chromat Path: H:\Pa14\MrT7\591v1\591v1_D05_D5_043.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
GTTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCTACC
TCGGGCTTGTCCCACATGTGTGCCACCTGTTTCTCCAGGCGTTGCGACAACATGATGTCGTCCGACCCGA
AGGGCACGATGAGATTTCCCCGGGCCCTGGCGATCGCCTCGTTGAGCGTTTTCGACAGTCCCTGGTTCTG
CTGGACCCTGAGGTCGAAGCCGTACTTCGCCTGGAGCCCGTGCAAAAGCTCGACGCTGCCATCGCTGGAA
CCATCGTCCACCACCAGCAGTTCCACGTTGCGGTACGTCTGCGCAGCCACGCTGGCGATGCTCGCTTCTA
TGTACTTTTCATGGTTATAGGAAGCGATGATCACGGAAACCAATGGCTGCGAACCATCATCGACGAGCGT
CATAACTACAACCTTACCTGTCCTTCACGTCCCCATTCAAAGCGCCAGACATGCATGAGCGATCCTGCGA
TCTTCATCGGTCCTACACCTCCCCCCAGCCCGGAAAAACAAGCTGAACAATAGCAAACACGCCTGAAGCC
CCAGCTCTTTTCGGCTGCTACTCTCAGGTAAAGATACGTCGCCAGGTCTTCTCTGCCNGGTCCGTGGACA
AGCTCCGGCTCGCGCAACGCGGGGCCGCATGGCCCTCGACGATACTCTGACCCACCATCGTCTAGCGGGG
CCTGGCCAAGATGGCCAGCGCCCTTGGCCCCTTCACGGCGATCAGTTTCGGCAAGGGCCATTGTCTTAGC
GCAAGGACCGCGAGGCTTTTCCTGGATAGTCCCCCCACCGAGCGATAAAGCTCGAATTTTCTTCCCGGTC
CCCGGAAACCCAGGGCCTCTTTTTCCCGGTCGCCCTGGTCTTACGCCAAAAATN
Blast against PA14
Processed Seqquence ID: 24004
5' Trim Position: 5 3' Trim Position: 651
Processed Sequence:
(Transposon Sequence in Red)		 
GTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCTACCTCGGG
CTTGTCCCACATGTGTGCCACCTGTTTCTCCAGGCGTTGCGACAACATGATGTCGTCCGACCCGAAGGGC
ACGATGAGATTTCCCCGGGCCCTGGCGATCGCCTCGTTGAGCGTTTTCGACAGTCCCTGGTTCTGCTGGA
CCCTGAGGTCGAAGCCGTACTTCGCCTGGAGCCCGTGCAAAAGCTCGACGCTGCCATCGCTGGAACCATC
GTCCACCACCAGCAGTTCCACGTTGCGGTACGTCTGCGCAGCCACGCTGGCGATGCTCGCTTCTATGTAC
TTTTCATGGTTATAGGAAGCGATGATCACGGAAACCAATGGCTGCGAACCATCATCGACGAGCGTCATAA
CTACAACCTTACCTGTCCTTCACGTCCCCATTCAAAGCGCCAGACATGCATGAGCGATCCTGCGATCTTC
ATCGGTCCTACACCTCCCCCCAGCCCGGAAAAACAAGCTGAACAATAGCAAACACGCCTGAAGCCCCAGC
TCTTTTCGGCTGCTACTCTCAGGTAAAGATACGTCGCCAGGTCTTCTCTGCCNGGTCCGTGGACAAGCTC
CGGCTCGCGCAACGCG
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
104957 104957 104958 104958
PA14 BLAST Hit ID: 104957       Show Transposon Map
Identities: 550/551 (99.81%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 85.14%
Query Start: 58 Query End: 608 Subject Start: 5889455 Subject End: 5890005 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5889455 PA14_66110 -
Raw Sequence Start: 5889393 PA14_66110 -
Extrapolated Insert Location: 5889456 PA14_66110 -
Default Offset Location: 5889456 PA14_66110 -
PAO1 BLAST Hit ID: 104958       Show Transposon Map
Identities: 541/551 (98.18%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 85.14%
Query Start: 58 Query End: 608 Subject Start: 5618057 Subject End: 5618607 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 5618057 PA5000 -
Raw Sequence Start: 5617995 PA5000 -
Extrapolated Insert Location: 5618058 PA5000 -
Default Offset Location: 5618058 PA5000 -
H PA14 Blast Hit H PAO1 Blast Hit