Mutant Report for Mutant ID: 56302
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 30847
Chromat Path: H:\Pa14\MrT7\587v1\587v1_D10_D10_080.ab1 Last Transposon Base: 61 Transposon Sequence
Match Quality: 6
Raw Sequence:
(Transposon Sequence in Red)		 
TGTAGTTTCGACCGAACAGGCATCATCTGCTGGCGTGGGCACAATTATCAGCCAACCTGTTAGCGGTGGC
CGACGGCTGGTCGGTGGAGTTTTGGCGCGACTACTGCGATGCGCACAACGTATTGCTGGTCGGCATGCGC
CATGGCTCGGTCACCCGCTACGGATTCGCCGAGGGCACCTACCGCTACGCCGACTATCTGTGCGGCAGCG
CCTGGGACATCGACGACACCCTGGCTTCCTCGGTGATGCCGCGCAACGGTTTCCTGCTGACCGGCAACGC
CTGGTGCGACGAGGTGTTCCGGCTGCCCGCGAGAACCCCGGCGGAGAACGCGCCGACCATCCTCTTCGCC
CCCACCTACAACCCCGAGATCAGCGCCGCCGTCCACCTCGGCGAGCGTGTGGTGGCGTTTGATCCGCAAG
GTCTATCCCGCGTCGCGTATCATCATCTAGGGGTCTGGACAGTACATTCGTCTTCTTTCTATTGTTTCAT
TTCTTTTTTCTCTCTCCTTTCTCCTCCTGACTCTTTTTCTCTCTTAGTTATTTTTCTTCTTCCTGTTTTC
GTCTTTTCTTACTCAATTTGTATCTTTTTTACTGTCTTCTCCCTTCATTATCCCCGCTCCCTTTTATAGT
CATATTGATTTTTTTCTTTCCTTTTGTGTTTTTTTCCTCCCTTCCCCCTCTACTCCCCCTCTCCTTGTTT
TCTCCTTTTTATCTTTTTTTTTTCTTGTGGTCTGGTCTGCGCCCTATTTTTTTTATTTTTTATCATCGCC
AGGTTTCGTTCCGTTTTACGTCTATCTCTCGTGGTTTAATATCGCCGCCCCTCCCCTCTTTTCTCTTCTT
TTTTTTCTTCTTTATCTTCTCCTCTTTCCTTCTCCT
Blast against PA14
Processed Seqquence ID: 23701
5' Trim Position: 39 3' Trim Position: 360
Processed Sequence:
(Transposon Sequence in Red)		 
CACAATTATCAGCCAACCTGTTAGCGGTGGCCGACGGCTGGTCGGTGGAGTTTTGGCGCGACTACTGCGA
TGCGCACAACGTATTGCTGGTCGGCATGCGCCATGGCTCGGTCACCCGCTACGGATTCGCCGAGGGCACC
TACCGCTACGCCGACTATCTGTGCGGCAGCGCCTGGGACATCGACGACACCCTGGCTTCCTCGGTGATGC
CGCGCAACGGTTTCCTGCTGACCGGCAACGCCTGGTGCGACGAGGTGTTCCGGCTGCCCGCGAGAACCCC
GGCGGAGAACGCGCCGACCATCCTCTTCGCCCCCACCTACA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
103888 103888 103889 103889
PA14 BLAST Hit ID: 103888       Show Transposon Map
Identities: 300/300 (100.00%) BLAST Bit Score: 595.0 Percent of Processed Sequence That Aligns: 93.15%
Query Start: 22 Query End: 321 Subject Start: 4471675 Subject End: 4471376 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4471675 PA14_50300 +
Raw Sequence Start: 4471735 PA14_50300 +
Extrapolated Insert Location: 4471674 PA14_50300 +
Default Offset Location: 4471672 PA14_50300 +
PAO1 BLAST Hit ID: 103889       Show Transposon Map
Identities: 298/300 (99.33%) BLAST Bit Score: 579.0 Percent of Processed Sequence That Aligns: 93.15%
Query Start: 22 Query End: 321 Subject Start: 1178253 Subject End: 1178552 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1178253 PA1091 +
Raw Sequence Start: 1178193 PA1091 +
Extrapolated Insert Location: 1178254 PA1091 +
Default Offset Location: 1178256 PA1091 +
H PA14 Blast Hit H PAO1 Blast Hit