Mutant Report for Mutant ID: 52904
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 27449
Chromat Path: H:\Pa14\MrT7\552v1\552v1_A08_A8_055.ab1 Last Transposon Base: 64 Transposon Sequence
Match Quality: 6
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCCGTTACGAACCGAATAGGTATAGTCTGCTGGCGTACCGGAAACTTATTAACCGACCTGATAAATAC
CGTAAGTACACGGCGTGCTTTTCTCGCACCTGGTCTGTCAGGCGGGCCACGAAGCCGCCGCCGCCGAGGA
TCTGGTTGCCCAGGTAGAGCGCCGCGTAGTCGGGATCCTGGCGGTCGATACCAAGCTGGGCCATCATCTG
CAGGCCTGGCCATGAGGGGAAGTCTATGTGCGTCAGGCCGGGCTTGGGCGTTTCCGGCTGGGCGGTCTTG
GCCAGCGCCTGGTCCTGGGGCACCGCCTTGAAGACTTCCGCGGAGATCGCTTCGGTCTCCTGCCTCTTAG
ATGTCTCCGATCAGCGTGATGACCACGTTGCCGGCGGTGTATGCTTTCTTGTGTAATCCCTGCTATTGCT
CCCGTTTGATCTTTGTAATGGATTTTTCTTTCCCGTCTCTGGCGTGCTCGTAGGGATTTTCTCTTTATAG
TTTTTTGACTCGTCCTGCTTGCCATTTTGTCGAGGTCTTGCTTTTGTTTTCCAACGGACCTGATATGTGT
CATGGTCAATGTTTATCTCCGCCCCCACCGCCCCTATGCTACCTCTCTTCTTCACTTGTTTCTTTCTTGC
TTCCTTTTTTCCCCTTTTTTTTCTTACTCTTCTGTTCTCTCCTTTTTTTGTTGTGTCTGTGTATATTTTT
TTTTTTGCTCTTCGTCGCTGCTTTTCTCCCCTGCTCTGTTTGTGCTGCGATTCCTTTCCCCTGCGACTCC
TCCTCTTGTGCATCTTTCGCTTGTGTCTACTGTCTCCCTGTTATTTCCCTCACCGTTCTCTCCCCGCCCC
GGTTTCCGCCGTCAGCGCGCCCGGTGTGCGCGCCACGC
Blast against PA14
Processed Seqquence ID: 21003
5' Trim Position: 80 3' Trim Position: 329
Processed Sequence:
(Transposon Sequence in Red)		 
CGGCGTGCTTTTCTCGCACCTGGTCTGTCAGGCGGGCCACGAAGCCGCCGCCGCCGAGGATCTGGTTGCC
CAGGTAGAGCGCCGCGTAGTCGGGATCCTGGCGGTCGATACCAAGCTGGGCCATCATCTGCAGGCCTGGC
CATGAGGGGAAGTCTATGTGCGTCAGGCCGGGCTTGGGCGTTTCCGGCTGGGCGGTCTTGGCCAGCGCCT
GGTCCTGGGGCACCGCCTTGAAGACTTCCGCGGAGATCG
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
94668 94668 94669 94669 94670 94670 94671 94671 94672 94672 94673 94673
PA14 BLAST Hit ID: 94668       Show Transposon Map
Identities: 109/115 (94.78%) BLAST Bit Score: 180.0 Percent of Processed Sequence That Aligns: 45.78%
Query Start: 7 Query End: 121 Subject Start: 429735 Subject End: 429849 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 429735 PA14_04870,PA14_04880* -,+
Raw Sequence Start: 429649 PA14_04870,PA14_04880* -,+
Extrapolated Insert Location: 429713 PA14_04870,PA14_04880* -,+
Default Offset Location: 429712 PA14_04870,PA14_04880* -,+
PA14 BLAST Hit ID: 94669       Show Transposon Map
Identities: 100/106 (94.33%) BLAST Bit Score: 163.0 Percent of Processed Sequence That Aligns: 42.17%
Query Start: 144 Query End: 249 Subject Start: 429872 Subject End: 429977 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 429872 PA14_04870,PA14_04880* -,+
Raw Sequence Start: 429649 PA14_04870,PA14_04880* -,+
Extrapolated Insert Location: 429713 PA14_04870,PA14_04880* -,+
Default Offset Location: 429712 PA14_04870,PA14_04880* -,+
PA14 BLAST Hit ID: 94670       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 8.03%
Query Start: 198 Query End: 218 Subject Start: 3087944 Subject End: 3087924 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3087944 PA14_34760*,PA14_34770 +,-
Raw Sequence Start: 3088221 PA14_34760*,PA14_34770 +,-
Extrapolated Insert Location: 3088157 PA14_34760*,PA14_34770 +,-
Default Offset Location: 3088158 PA14_34760*,PA14_34770 +,-
PAO1 BLAST Hit ID: 94671       Show Transposon Map
Identities: 109/115 (94.78%) BLAST Bit Score: 180.0 Percent of Processed Sequence That Aligns: 45.78%
Query Start: 7 Query End: 121 Subject Start: 414987 Subject End: 415101 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 414987 PA0371 -
Raw Sequence Start: 414901 PA0371 -
Extrapolated Insert Location: 414965 PA0371 -
Default Offset Location: 414964 PA0371 -
PAO1 BLAST Hit ID: 94672       Show Transposon Map
Identities: 99/106 (93.39%) BLAST Bit Score: 155.0 Percent of Processed Sequence That Aligns: 42.17%
Query Start: 144 Query End: 249 Subject Start: 415124 Subject End: 415229 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 415124 PA0371 -
Raw Sequence Start: 414901 PA0371 -
Extrapolated Insert Location: 414965 PA0371 -
Default Offset Location: 414964 PA0371 -
PAO1 BLAST Hit ID: 94673       Show Transposon Map
Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 8.03%
Query Start: 198 Query End: 218 Subject Start: 2547887 Subject End: 2547907 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2547887 PA2309 -
Raw Sequence Start: 2547610 PA2309 -
Extrapolated Insert Location: 2547674 PA2309 -
Default Offset Location: 2547673 PA2309 -
H PA14 Blast Hit H PAO1 Blast Hit