Mutant Report for Mutant ID: 41694
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 25455
Chromat Path: H:\Pa14\MrT7\435v1\435v1_C06_C6_040.ab1 Last Transposon Base: 25 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
CCGGGGACTTATCAGCCAACCTGTTAGCCCTGCGCGTAGATGCTGGCGATCGCCTCGTCCAGATACTTCG
AGATATTGAAACAGGCGCAAACGACGGAAACGAGCGGACGCATTTCTTCCTCCCCAGCCCCGGATCCGGG
ACTCGGCACAAACCGATAACCTGGCTGCCCGTCGGAAGGAAACCGTGCCATGCAGCTCGGCCACATCTTC
TGCGCGCCTGGGCACACCGGGATACTATACTAATAAGATTCGTACTCTTGCATTTTTGGCCATCCCTCCC
CCCTCCTCCCTCCCTTTTCCCTTATCCTCTTTTTTTTTTCTTTTTTTTCTTTCTTTTTTCTTTTCGCTTT
TTGGTTGTTTGTTTTTTGTTTTTCTGCTCATCGCCCTTTTTTTTTTTGTTTTTTTCTTTCTTTTTATTTT
TTTTTTTTTTTTTTTTTTTTTCTCTTTTTTATCTTTCTCCTTCCCCCCATTTTTTTATTTCTCCTTTTTT
CTCCTTTATTTCCACTTTTCCCTCCCCTCCTTCCCTACTCTCCTCCTTCTTACATTCTAAGTTTCTTTTT
TCTACTTTTATCCTATTCTTTCTTTTATCTTAATCATTTTTATTATTAATATATATTCACTACTCCTTTC
TTTTTGTCGCTTCTGATTTTCTCTTTATGCACATTCTTTGCTCCCTCAATCCATTCCACTTCCTCCATCT
ACTCAATATAATCCTCATTTTTTCCTTTTTTAATCTCCTCCTATCTATTTATCTATTTTATCATCTTTTT
TTATCTTCTATTCACTTTAAATTACTATTACTAAATCCAATTTTATCCATATCATTTTTTATTTATTTTA
TTACTTACCTCCATTTTTTACCATTTTTTCTCTCTTTCTAA
Blast against PA14
Processed Seqquence ID: 19384
5' Trim Position: 1 3' Trim Position: 213
Processed Sequence:
(Transposon Sequence in Red)		 
CGGGGACTTATCAGCCAACCTGTTAGCCCTGCGCGTAGATGCTGGCGATCGCCTCGTCCAGATACTTCGA
GATATTGAAACAGGCGCAAACGACGGAAACGAGCGGACGCATTTCTTCCTCCCCAGCCCCGGATCCGGGA
CTCGGCACAAACCGATAACCTGGCTGCCCGTCGGAAGGAAACCGTGCCATGCAGCTCGGCCACATCTTCT
GC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
87897 87897 87898 87898 87899 87899 87900 87900
PA14 BLAST Hit ID: 87897       Show Transposon Map
Identities: 181/181 (100.00%) BLAST Bit Score: 359.0 Percent of Processed Sequence That Aligns: 84.91%
Query Start: 24 Query End: 204 Subject Start: 4552454 Subject End: 4552634 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4552454 PA14_51220 -
Raw Sequence Start: 4552430 PA14_51220 -
Extrapolated Insert Location: 4552455 PA14_51220 -
Default Offset Location: 4552493 PA14_51220 -
PA14 BLAST Hit ID: 87898       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 10.85%
Query Start: 30 Query End: 53 Subject Start: 140977 Subject End: 140954 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 140977 PA14_01500 +
Raw Sequence Start: 141007 PA14_01500 +
Extrapolated Insert Location: 140982 PA14_01500 +
Default Offset Location: 140944 PA14_01500 +
PAO1 BLAST Hit ID: 87899       Show Transposon Map
Identities: 181/181 (100.00%) BLAST Bit Score: 359.0 Percent of Processed Sequence That Aligns: 84.91%
Query Start: 24 Query End: 204 Subject Start: 1097477 Subject End: 1097297 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1097477 PA1014 -
Raw Sequence Start: 1097501 PA1014 -
Extrapolated Insert Location: 1097476 PA1014 -
Default Offset Location: 1097438 PA1014 -
PAO1 BLAST Hit ID: 87900       Show Transposon Map
Identities: 23/24 (95.83%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 10.85%
Query Start: 30 Query End: 53 Subject Start: 143063 Subject End: 143040 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 143063 PA0123 +
Raw Sequence Start: 143093 PA0123 +
Extrapolated Insert Location: 143068 PA0123 +
Default Offset Location: 143030 PA0123 +
H PA14 Blast Hit H PAO1 Blast Hit