Mutant Report for Mutant ID: 41677
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 25438
Chromat Path: H:\Pa14\MrT7\435v1\435v1_B01_B1_011.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
TGTCAGTTTGTATACGACTCACTATAGGGGTCTAGAGACCGGAGACTTATCAGCCAACCTGTTATACCGG
TCCCTACTGTGTCTCGCAGGATTGCCCAGGAGGTCTTCACCGAGGCCCTGCATGCCGAACTGAAGCCGTT
CGGCATCCAGGTCGGCACCATCAATCCCGGCCCGTACCAGACCGGCTTCAACGATCGCATGATGGAAACC
TGGAAACGCTGGTACGACCCGCAGCGCCACTTCACCGACCACAGCGGCGTGCGCTTTCCCTTCGAGCAGT
ACGATCCGGAGGAAATGATCGCGCGGATGGTCGAGCTGGCCGAGGCCGACGAAGGACCGTTCCGCACCCT
GTTGCCGGAAGCTTTCGTCGAGGTGGTGAAGGAACATCATTTGCTGTCCTGGCAATGCCCCCCGTTCCCT
CGCCCTTGTTGTGTCTTTGAGGGTTTTTTTCTTATTTTATGAATGTTTATTTTTTTATTTGCTGCGTTTT
CGTTTCCCCTTTCCCCCGGATATATTTCCAATTCCACCACTCTACCACTCTCTCTCCTGACCCTCCTCCC
GTCTTCTTCTTCCTCTACTCCTTCTAAACGTCCCTTTCCCCCTCCGCACGCCCATTTTTTCTACTTTCCA
TCGTATCGTTTTACTATCATTCTATGCCTATCTGCTCTCTCACCACTACTTACTTCCTATTTACCTGTAT
ACCCCCCTTTACCCCTCCTCACCCTCTACACTCTTTCTTTCCTCTCCTCAGTCCTCCCCAGTTGTCCTTC
CCTTTCCCTTTCTAATCTGATTGAATAATGTTACATATTTACGCCTAGTGCATCACACTGCTCCCCCCCT
TCCCCTACTCTACTCTTCTATATTCTCTATTACTCTTCTTTTTTATTTTTTTCTTCTTCCTTTC
Blast against PA14
Processed Seqquence ID: 19370
5' Trim Position: 18 3' Trim Position: 373
Processed Sequence:
(Transposon Sequence in Red)		 
TCACTATAGGGGTCTAGAGACCGGAGACTTATCAGCCAACCTGTTATACCGGTCCCTACTGTGTCTCGCA
GGATTGCCCAGGAGGTCTTCACCGAGGCCCTGCATGCCGAACTGAAGCCGTTCGGCATCCAGGTCGGCAC
CATCAATCCCGGCCCGTACCAGACCGGCTTCAACGATCGCATGATGGAAACCTGGAAACGCTGGTACGAC
CCGCAGCGCCACTTCACCGACCACAGCGGCGTGCGCTTTCCCTTCGAGCAGTACGATCCGGAGGAAATGA
TCGCGCGGATGGTCGAGCTGGCCGAGGCCGACGAAGGACCGTTCCGCACCCTGTTGCCGGAAGCTTTCGT
CGAGG
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
87843 87843 87844 87844
PA14 BLAST Hit ID: 87843       Show Transposon Map
Identities: 302/311 (97.10%) BLAST Bit Score: 537.0 Percent of Processed Sequence That Aligns: 87.32%
Query Start: 45 Query End: 355 Subject Start: 4192144 Subject End: 4191835 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4192144 PA14_47060 +
Raw Sequence Start: 4192206 PA14_47060 +
Extrapolated Insert Location: 4192143 PA14_47060 +
Default Offset Location: 4192143 PA14_47060 +
PAO1 BLAST Hit ID: 87844       Show Transposon Map
Identities: 274/281 (97.50%) BLAST Bit Score: 502.0 Percent of Processed Sequence That Aligns: 78.87%
Query Start: 75 Query End: 355 Subject Start: 1442524 Subject End: 1442804 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1442524 PA1330 +
Raw Sequence Start: 1442432 PA1330 +
Extrapolated Insert Location: 1442495 PA1330 +
Default Offset Location: 1442495 PA1330 +
H PA14 Blast Hit H PAO1 Blast Hit