Mutant Report for Mutant ID: 36078
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 19839
Chromat Path: H:\Pa14\MrT7\376v1\376v1_G06_G6_050.ab1 Last Transposon Base: 59 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
CCGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCATGAGCAC
CTGCCGCGCGAATACTGCGTGCAGGCCGGCGACAGCGATCACTACCTGCACGACCGGCTGGCTTTCGAGG
AAGGCCTGGTCTACTACTTCCGCTTCGACGAACACCGCCACACCCTGGTCTGCGCCGACCGGGTCAACGT
CCAGGAGCGTATCGCCGGCGGCCCGGTACTGTTCAGCGCCCAGCCGGAGGGCGACAACCCGCAGCCGGTG
CTTCATTCCTTCCGCTACAGCGAAAATGTTCGTACGGCTCGACAGACCCAGCGGGACTACAGCTTCAAGC
GCCCGACATACGACCAGGAGCCATCATCTGAGGGGCCTGGCCAANNNNNNTNNNNNNTNCNNNNTTCNNG
CGCNCGTNCTCTCCCGTTCGCTTCTCGCTGGGCGGTCGTGTGCCGGTCTTTTCGCTGGGGCCGCCTTTTT
ATGAAATCTTCGAAGATCACGTATGGAATTGTGAATCAACGACTACCCTTGTCTGATCCTGGGCAACGTA
ATTGCACTAGAAAGTACATCTCTATTCGACTACAGCGCCGGGGGGAGCCAGTTCCAGTGGTTCATTCGTG
AACCCAGAATCCCAACCAGAAAGAAAGAGACCAAAATAAACCCTTTTGTGGTATATTTCAACTCGCCCCT
CAAATTTGGGTCCTAAAAAATCTAGTAGCGCCCCGAAACCATATCATACTATACTCTTTTGTTTATCTGA
TATCTGATCAACATCCATACTCCACGTGATAAAATAAATTAAGAAAAGTGAGCGGCAGTGTAAAATTTTA
TCCTCTTGGGACTCTGTGA
Blast against PA14
Processed Seqquence ID: 14562
5' Trim Position: 2 3' Trim Position: 380
Processed Sequence:
(Transposon Sequence in Red)		 
GTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCATGAGCACCT
GCCGCGCGAATACTGCGTGCAGGCCGGCGACAGCGATCACTACCTGCACGACCGGCTGGCTTTCGAGGAA
GGCCTGGTCTACTACTTCCGCTTCGACGAACACCGCCACACCCTGGTCTGCGCCGACCGGGTCAACGTCC
AGGAGCGTATCGCCGGCGGCCCGGTACTGTTCAGCGCCCAGCCGGAGGGCGACAACCCGCAGCCGGTGCT
TCATTCCTTCCGCTACAGCGAAAATGTTCGTACGGCTCGACAGACCCAGCGGGACTACAGCTTCAAGCGC
CCGACATACGACCAGGAGCCATCATCTG
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
65864 65864 65865 65865 65866 65866 65867 65867 65868 65868 65869 65869
PA14 BLAST Hit ID: 65864       Show Transposon Map
Identities: 308/313 (98.40%) BLAST Bit Score: 581.0 Percent of Processed Sequence That Aligns: 82.54%
Query Start: 57 Query End: 369 Subject Start: 287867 Subject End: 287555 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 287867 PA14_03220,PA14_03230* +,-
Raw Sequence Start: 287925 PA14_03220,PA14_03230* +,-
Extrapolated Insert Location: 287866 PA14_03220,PA14_03230* +,-
Default Offset Location: 287862 PA14_03220,PA14_03230* +,-
PA14 BLAST Hit ID: 65865       Show Transposon Map
Identities: 304/313 (97.12%) BLAST Bit Score: 549.0 Percent of Processed Sequence That Aligns: 82.54%
Query Start: 57 Query End: 369 Subject Start: 4001194 Subject End: 4001506 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4001194 PA14_44900 +
Raw Sequence Start: 4001136 PA14_44900 +
Extrapolated Insert Location: 4001195 PA14_44900 +
Default Offset Location: 4001199 PA14_44900 +
PA14 BLAST Hit ID: 65866       Show Transposon Map
Identities: 29/32 (90.62%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 8.20%
Query Start: 75 Query End: 106 Subject Start: 3019167 Subject End: 3019136 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3019167 PA14_33950*,PA14_33960 -,+
Raw Sequence Start: 3019243 PA14_33950*,PA14_33960 -,+
Extrapolated Insert Location: 3019184 PA14_33950*,PA14_33960 -,+
Default Offset Location: 3019180 PA14_33950*,PA14_33960 -,+
PAO1 BLAST Hit ID: 65867       Show Transposon Map
Identities: 307/313 (98.08%) BLAST Bit Score: 573.0 Percent of Processed Sequence That Aligns: 82.54%
Query Start: 57 Query End: 369 Subject Start: 1643620 Subject End: 1643308 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1643620 PA1511 +
Raw Sequence Start: 1643678 PA1511 +
Extrapolated Insert Location: 1643619 PA1511 +
Default Offset Location: 1643615 PA1511 +
PAO1 BLAST Hit ID: 65868       Show Transposon Map
Identities: 307/313 (98.08%) BLAST Bit Score: 573.0 Percent of Processed Sequence That Aligns: 82.54%
Query Start: 57 Query End: 369 Subject Start: 296456 Subject End: 296144 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 296456 PA0262 +
Raw Sequence Start: 296514 PA0262 +
Extrapolated Insert Location: 296455 PA0262 +
Default Offset Location: 296451 PA0262 +
PAO1 BLAST Hit ID: 65869       Show Transposon Map
Identities: 31/34 (91.17%) BLAST Bit Score: 44.1 Percent of Processed Sequence That Aligns: 8.73%
Query Start: 75 Query End: 108 Subject Start: 2624627 Subject End: 2624660 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2624627 PA2373 +
Raw Sequence Start: 2624551 PA2373 +
Extrapolated Insert Location: 2624610 PA2373 +
Default Offset Location: 2624614 PA2373 +
H PA14 Blast Hit H PAO1 Blast Hit