Mutant Report 33583

Mutant Report for Mutant ID: `33583`

Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04

Raw Sequence ID: 17359

Chromat Path: H:\Pa14\MrT7\350v1\350v1_G07_G7_056.ab1 Last Transposon Base: 62 Transposon Sequence
Match Quality: 0

Raw Sequence:
(Transposon Sequence in Red)

GTTCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGGTCAGG
TTCGCGCCGCCCTCCTCCTCCGGCAGCAGCTCCAGCGACGACAGCGGTTCGTTCATTGACGGATCACCAC
GGCCGTCAGGTTGTCCCGCGCGGCGCCGCTCAGGGCGCCGTCGAACAGCCGCTCCACCGCCACCTGCGGC
GATGCCAGGCTCAACGCGCGGCCCAGCGCATCGGCGCTCAGCTCTTCGTAGAGCCCGTCGCTGCAGAGCA
GGAAGACATCACCGGGATGCACCTCCAGTTCCAACACGTCCAGCACCAGTTGTTCGCTGGCGCCGACGGC
ACGGGTCAGCGCGCGCGCCGAGGGATAGGCGCGCGCCTGCTCGATACCCATCCGCCCCATCATCTGCAGG
CCTGGCCACAGTGAGTGGTCGTGGGACAACTGGAACAGCCGCTGTCCGCGCCCCAGTTACAACTACTGTC
GCCGACCCATACACAGGCTCGTCATCGAACTCTACACCTGAAAATCGGCGTGGGTAGTGATATAAAAAAT
GGTCGAAATAAAAAATGAGATGAAGCGGTCTTATACTACCGATTCTTGGCATGTATCTGGAAAAATAATG
TTCTGGTACCAAATTGGGGGATTTTTATGTAACATAATCAAAAGTAAAAATACATAGTCGATGATATCCA
ATCGGGCTTGGTATTCAGACAATTTTGCACTCAATCTTTATTGTCGTCGTATATAAATAATTCTTAAACA
TTTTGTCCAAATATTTGGTTTTTTAAATCATCTCACCTAACTTTTTTTATTATCTGTTTAATATTCGTCG
TGTTTAATTATTTATAAATGCTGACGTCGGGGTAATATATATTCGTCTTTAATAATTTTATATAATTN

Blast against PA14

Processed Seqquence ID: 12336

5' Trim Position: 3 3' Trim Position: 427

Processed Sequence:
(Transposon Sequence in Red)

CAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGGTCAGGTTC
GCGCCGCCCTCCTCCTCCGGCAGCAGCTCCAGCGACGACAGCGGTTCGTTCATTGACGGATCACCACGGC
CGTCAGGTTGTCCCGCGCGGCGCCGCTCAGGGCGCCGTCGAACAGCCGCTCCACCGCCACCTGCGGCGAT
GCCAGGCTCAACGCGCGGCCCAGCGCATCGGCGCTCAGCTCTTCGTAGAGCCCGTCGCTGCAGAGCAGGA
AGACATCACCGGGATGCACCTCCAGTTCCAACACGTCCAGCACCAGTTGTTCGCTGGCGCCGACGGCACG
GGTCAGCGCGCGCGCCGAGGGATAGGCGCGCGCCTGCTCGATACCCATCCGCCCCATCATCTGCAGGCCT
GGCC

Blast against PA14

BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence

PA14 BLAST Hit ID: 55740 Show Transposon Map

Identities: 344/345 (99.71%) BLAST Bit Score: 676.0 Percent of Processed Sequence That Aligns: 81.13%

Query Start: 59 Query End: 403 Subject Start: 3811425 Subject End: 3811769 Tn Insert Strand: +

Location Name	Genome Location	ORF(*=unsupported)	ORF Orientation Relative to Query Sequence
Subject Start:	`3811425`	`PA14_42880`	`-`
Raw Sequence Start:	`3811364`	`PA14_42880`	`-`
Extrapolated Insert Location:	`3811426`	`PA14_42880`	`-`
Default Offset Location:	`3811427`	`PA14_42880`	`-`

PA14 BLAST Hit ID: 55741 Show Transposon Map

Identities: 25/26 (96.15%) BLAST Bit Score: 44.1 Percent of Processed Sequence That Aligns: 5.90%

Query Start: 72 Query End: 97 Subject Start: 4378791 Subject End: 4378766 Tn Insert Strand: -

Location Name	Genome Location	ORF(*=unsupported)	ORF Orientation Relative to Query Sequence
Subject Start:	`4378791`	`PA14_49290`	`-`
Raw Sequence Start:	`4378865`	`PA14_49290`	`-`
Extrapolated Insert Location:	`4378803`	`PA14_49290`	`-`
Default Offset Location:	`4378802`	`PA14_49290`	`-`

PA14 BLAST Hit ID: 55742 Show Transposon Map

Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 4.72%

Query Start: 262 Query End: 282 Subject Start: 87896 Subject End: 87916 Tn Insert Strand: +

Location Name	Genome Location	ORF(*=unsupported)	ORF Orientation Relative to Query Sequence
Subject Start:	`87896`	`PA14_00880*,PA14_00890`	`-,-`
Raw Sequence Start:	`87632`
Extrapolated Insert Location:	`87694`
Default Offset Location:	`87695`

PAO1 BLAST Hit ID: 55743 Show Transposon Map

Identities: 337/345 (97.68%) BLAST Bit Score: 620.0 Percent of Processed Sequence That Aligns: 81.13%

Query Start: 59 Query End: 403 Subject Start: 1823390 Subject End: 1823046 Tn Insert Strand: -

Location Name	Genome Location	ORF(*=unsupported)	ORF Orientation Relative to Query Sequence
Subject Start:	`1823390`	`PA1671`	`-`
Raw Sequence Start:	`1823451`	`PA1671`	`-`
Extrapolated Insert Location:	`1823389`	`PA1671`	`-`
Default Offset Location:	`1823388`	`PA1671`	`-`

PAO1 BLAST Hit ID: 55744 Show Transposon Map

Identities: 21/21 (100.00%) BLAST Bit Score: 42.1 Percent of Processed Sequence That Aligns: 4.72%

Query Start: 262 Query End: 282 Subject Start: 90251 Subject End: 90271 Tn Insert Strand: +

Location Name	Genome Location	ORF(*=unsupported)	ORF Orientation Relative to Query Sequence
Subject Start:	`90251`	`PA0075`	`-`
Raw Sequence Start:	`89987`	`PA0074`	`-`
Extrapolated Insert Location:	`90049`	`PA0074`	`-`
Default Offset Location:	`90050`	`PA0074`	`-`

H PA14 Blast Hit H PAO1 Blast Hit

Mutant Report for Mutant ID: 33583

Mutant Report for Mutant ID: `33583`