Mutant Report for Mutant ID: 33483
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 17259
Chromat Path: H:\Pa14\MrT7\349v1\349v1_G03_G3_023.ab1 Last Transposon Base: 25 Transposon Sequence
Match Quality: 1
Raw Sequence:
(Transposon Sequence in Red)		 
CCGGGGACTTATCAGCCAACCTGTTCGGTGTCGCCCGGCACTTACAGCGTCGACATTCATTTGAACCAGA
TCATGATCCGGCGCGAAGATGTCGTGTTCAGTGCCGATCCCGACACCGGAAGCGTTCGCCCGATCATTCG
GATGGGCCTGCTCAAGGAGATTGGGGTCGATATCGCCCGGCTGACTCGGGACAAGCTCATTCCGGCCAAC
CTGGAAAACAATACGCCCCTGAATGTCGCCGAACTCATCCCCGGTGCATCCGTAGAGTTCGACGTCAACA
GCCTTTCCCTGCTGGTCAGCATTCCACAGCTGTATGTACAGCGGCACTCGCGTGGCTATGTGGATCCCTC
TCTTTGGGATGACGGCGTCACCGCGTTGTTTTCCAACTACCAGGCCAACTTCACGCGCAACACCAATTTT
GGCCAGAACAGCGATTACCGCTACCTGGGTCTGCGCAACGGCTTCAACCTGTTTGGCTGGCGCCTGCGCA
ACGACTCTTCCCTGAACGGAGGTACCGGCATGCGCAACAAGTTCAGTAGCAACCGTACCTATGTGGAACG
TGACATTCGTGCCCTGAAGGGGACCTTGTCCCTCGGGGAGTTGTATACCCTCCCGCTCAGGGGGATGCTT
TTCAAAGCGTGCGTATGCCCGGCGTGCAAACTGGAATCCCATATCGGCATGTTGCCCGACAACGAGATCA
ACCTATTCCCCGGCGGAGCCTCACTGTTCGGGGGTTTGGAAAAAATAAAATAATATGTAAAATAATAACA
AAGATCATTAGAGAGTGTTTTCATCACATCTTGCTATGCAGGATGTATAGCATTATCATAATACAAAACA
ATAATTTTCCAACCTTT
Blast against PA14
Processed Seqquence ID: 12248
5' Trim Position: 1 3' Trim Position: 537
Processed Sequence:
(Transposon Sequence in Red)		 
CGGGGACTTATCAGCCAACCTGTTCGGTGTCGCCCGGCACTTACAGCGTCGACATTCATTTGAACCAGAT
CATGATCCGGCGCGAAGATGTCGTGTTCAGTGCCGATCCCGACACCGGAAGCGTTCGCCCGATCATTCGG
ATGGGCCTGCTCAAGGAGATTGGGGTCGATATCGCCCGGCTGACTCGGGACAAGCTCATTCCGGCCAACC
TGGAAAACAATACGCCCCTGAATGTCGCCGAACTCATCCCCGGTGCATCCGTAGAGTTCGACGTCAACAG
CCTTTCCCTGCTGGTCAGCATTCCACAGCTGTATGTACAGCGGCACTCGCGTGGCTATGTGGATCCCTCT
CTTTGGGATGACGGCGTCACCGCGTTGTTTTCCAACTACCAGGCCAACTTCACGCGCAACACCAATTTTG
GCCAGAACAGCGATTACCGCTACCTGGGTCTGCGCAACGGCTTCAACCTGTTTGGCTGGCGCCTGCGCAA
CGACTCTTCCCTGAACGGAGGTACCGGCATGCGCAACAAGTTCAGT
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
55322 55322 55323 55323 55324 55324 55325 55325
PA14 BLAST Hit ID: 55322       Show Transposon Map
Identities: 512/513 (99.80%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 95.52%
Query Start: 24 Query End: 536 Subject Start: 4574971 Subject End: 4574459 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4574971 PA14_51450 +
Raw Sequence Start: 4574995 PA14_51450 +
Extrapolated Insert Location: 4574970 PA14_51450 +
Default Offset Location: 4574932 PA14_51450 +
PA14 BLAST Hit ID: 55323       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 3.54%
Query Start: 473 Query End: 492 Subject Start: 3905397 Subject End: 3905378 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3905397 PA14_43850 +
Raw Sequence Start: 3905870 PA14_43850 +
Extrapolated Insert Location: 3905845 PA14_43850 +
Default Offset Location: 3905807 PA14_43850 +
PAO1 BLAST Hit ID: 55324       Show Transposon Map
Identities: 494/513 (96.29%) BLAST Bit Score: 866.0 Percent of Processed Sequence That Aligns: 95.52%
Query Start: 24 Query End: 536 Subject Start: 1074961 Subject End: 1075473 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1074961 PA0994 +
Raw Sequence Start: 1074937 PA0994 +
Extrapolated Insert Location: 1074962 PA0994 +
Default Offset Location: 1075000 PA0994 +
PAO1 BLAST Hit ID: 55325       Show Transposon Map
Identities: 20/20 (100.00%) BLAST Bit Score: 40.1 Percent of Processed Sequence That Aligns: 3.54%
Query Start: 473 Query End: 492 Subject Start: 1738111 Subject End: 1738130 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1738111 PA1596 +
Raw Sequence Start: 1737638 PA1596 +
Extrapolated Insert Location: 1737663 PA1596 +
Default Offset Location: 1737701 PA1596 +
H PA14 Blast Hit H PAO1 Blast Hit