Mutant Report for Mutant ID: 33176
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 16952
Chromat Path: H:\Pa14\MrT7\346v1\346v1_E08_E8_067.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATTCTTG
CTGAACCCGCTCTCTGGAAACCTGATCCGGTGTTTTCATCATTTTCCAATCGATCACTTGGGCAACTCCA
CGGTTCCGTCTTCAACGATATGGATGGGGACAGGGAATTTGGCAGCTTCGCTGTAGCTCCCCCGGATGGG
AAACACCAATGTCAGGCATATCTCTCCGCCTGCCCTGGTAACCCTCCCGGAAAAAGGCCCCTCATGGATG
GCAGAGGCTGGCAGATATCCCCCCTCCGGTAACTGCGAGAAGTCGTACATGACACCCGAGACGGCTATCG
AATCTCCGGAAACCATGACATCCATATCAGTCGAGTCCTCGGGCAGGACTACAACCGGACTGAGTTCGAT
ACGCATCAGTACCATCATCTGCAAGCCTGGACAGTAATGGTGACGTTAGCCCCCAGTGCCTGCGCCGTCG
CGAAAGAAACAGAGCTGCTACCGTTGAGATAGGCTCCACGAACCCCCTGCCCTGCAGTGGCAAGCCATCC
GCTGGAAGGAATCCAATTGCAGGTCACCCCGGTATTGAAGCCCGTAACGAAAGCAGCTGGGAGCTGTATC
ACGTTAGCTCCAAACAAAAGGGCCGGTTTCGGATCTTGCCGCTGCCATCTGGGTACCATCCGCAAAAGTG
AACGTTCCAGTCGATTGAAAGTTGTCCCAGTTTTCCATAAAAGACCCCGGGGGGGCTTACCCGGAACAAA
GGCGCCTCTTTTACGGTCCCCACCAAATTTGGCTGGCAGTAAAAAAACCATAACCCAATTCCCTTCCGGG
TTTTCGATTTGGTACAAGGTCCCTGAATACAAACATCCTGGGAAGGGTAGGAAATTTCTCCAAAGAAAGA
AACCGGTATATAGCCG
Blast against PA14
Processed Seqquence ID: 12012
5' Trim Position: 3 3' Trim Position: 640
Processed Sequence:
(Transposon Sequence in Red)		 
TCAGTTTGTATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTATTCTTGCTG
AACCCGCTCTCTGGAAACCTGATCCGGTGTTTTCATCATTTTCCAATCGATCACTTGGGCAACTCCACGG
TTCCGTCTTCAACGATATGGATGGGGACAGGGAATTTGGCAGCTTCGCTGTAGCTCCCCCGGATGGGAAA
CACCAATGTCAGGCATATCTCTCCGCCTGCCCTGGTAACCCTCCCGGAAAAAGGCCCCTCATGGATGGCA
GAGGCTGGCAGATATCCCCCCTCCGGTAACTGCGAGAAGTCGTACATGACACCCGAGACGGCTATCGAAT
CTCCGGAAACCATGACATCCATATCAGTCGAGTCCTCGGGCAGGACTACAACCGGACTGAGTTCGATACG
CATCAGTACCATCATCTGCAAGCCTGGACAGTAATGGTGACGTTAGCCCCCAGTGCCTGCGCCGTCGCGA
AAGAAACAGAGCTGCTACCGTTGAGATAGGCTCCACGAACCCCCTGCCCTGCAGTGGCAAGCCATCCGCT
GGAAGGAATCCAATTGCAGGTCACCCCGGTATTGAAGCCCGTAACGAAAGCAGCTGGGAGCTGTATCACG
TTAGCTC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
54256 54256 54257 54257
PA14 BLAST Hit ID: 54256       Show Transposon Map
Identities: 565/579 (97.58%) BLAST Bit Score: 999.9 Percent of Processed Sequence That Aligns: 90.58%
Query Start: 60 Query End: 637 Subject Start: 713876 Subject End: 713299 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 713876 PA14_08330 -
Raw Sequence Start: 713938 PA14_08330 -
Extrapolated Insert Location: 713875 PA14_08330 -
Default Offset Location: 713875 PA14_08330 -
PAO1 BLAST Hit ID: 54257       Show Transposon Map
Identities: 560/579 (96.71%) BLAST Bit Score: 981.0 Percent of Processed Sequence That Aligns: 90.58%
Query Start: 60 Query End: 637 Subject Start: 702892 Subject End: 702315 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 702892 PA0648 -
Raw Sequence Start: 702954 PA0648 -
Extrapolated Insert Location: 702891 PA0648 -
Default Offset Location: 702891 PA0648 -
H PA14 Blast Hit H PAO1 Blast Hit