Mutant Report for Mutant ID: 32431
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 15823
Chromat Path: H:\Pa14\MrT7\338v1\338v1_G07_G7_054.ab1 Last Transposon Base: 57 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
CGTTTGATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCTCGGCCTCGG
CAGCAATGTCGAGCGTGAGCGCCACCTGCGTGCCGGCCTCGATGCGCTGGCCGGTTTCCTTCATGACCCG
CGTTGCTCGCCGGTGTTCGAGAGCGAGCCGGTGGGTATCAAGAGCGGGCCGTTCTTCAACCTGGTGGTGT
CTGCCCGCACCGATCTGCCGCTGGCCGAACTGAGCCTGCGACTCAAGCACATCGAGGCCGACAATGGCCG
CTATGCGCCAGAGCGCAAGGGACTGCCGCTGGATATCGACGTGCTGTTCTACGGCGACCTGGGGGGCGAT
TTCGATGGCCTGGTGCTGCCGCGCGCCGATAGTGCTGAAGAATGCTTTCGTGCTCTGGCCCTTGTCCGAT
CTGGCGCCTAACCTGGAGCATCCGGTTCGCCCGCCACCCGCTGGGCGATTTTCTGTCTGATGACGAGGAT
CCAGCAAAATATCTGGACGGCGCCATTCCAATGGCTGGCCCGAGCCTTGACTCCCTAAACACTGCTTCAT
TGCTTTCCCCGTCTTTCTTGGTAGGCTTGCAATGCAGTGAGGCTCGTACCCTACCCCTACTTTGCCAGAT
TATGCCCCTATAAACCCCTTTTCCCTCCCTGCTTTACTCTAACAGTTTGTGATATCCTGTTCTCACCCTA
TATAAGATGCCGTCCATTGATAATCCCCAACAGTATCCAGGACAAAGAAGTATTGTAGCTTTTTTATTTT
TATGTAAGCATATAAATCCTATTAAACCCATATCCGCAGAAGATTCCCCGCCACATACTTATACTTCCAA
TGTATCTACTATTACTTATACGCTCTCTTC
Blast against PA14
Processed Seqquence ID: 11167
5' Trim Position: 7 3' Trim Position: 425
Processed Sequence:
(Transposon Sequence in Red)		 
TACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACCTCGGCCTCGGCAGCAAT
GTCGAGCGTGAGCGCCACCTGCGTGCCGGCCTCGATGCGCTGGCCGGTTTCCTTCATGACCCGCGTTGCT
CGCCGGTGTTCGAGAGCGAGCCGGTGGGTATCAAGAGCGGGCCGTTCTTCAACCTGGTGGTGTCTGCCCG
CACCGATCTGCCGCTGGCCGAACTGAGCCTGCGACTCAAGCACATCGAGGCCGACAATGGCCGCTATGCG
CCAGAGCGCAAGGGACTGCCGCTGGATATCGACGTGCTGTTCTACGGCGACCTGGGGGGCGATTTCGATG
GCCTGGTGCTGCCGCGCGCCGATAGTGCTGAAGAATGCTTTCGTGCTCTGGCCCTTGTCCGATCTGGC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
50782 50782 50783 50783
PA14 BLAST Hit ID: 50782       Show Transposon Map
Identities: 365/369 (98.91%) BLAST Bit Score: 692.0 Percent of Processed Sequence That Aligns: 88.04%
Query Start: 50 Query End: 418 Subject Start: 654255 Subject End: 654622 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 654255 PA14_07600 +
Raw Sequence Start: 654199 PA14_07590 +
Extrapolated Insert Location: 654256 PA14_07600 +
Default Offset Location: 654262 PA14_07600 +
PAO1 BLAST Hit ID: 50783       Show Transposon Map
Identities: 362/369 (98.10%) BLAST Bit Score: 668.0 Percent of Processed Sequence That Aligns: 88.04%
Query Start: 50 Query End: 418 Subject Start: 641435 Subject End: 641802 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 641435 PA0583 +
Raw Sequence Start: 641379 PA0582 +
Extrapolated Insert Location: 641436 PA0583 +
Default Offset Location: 641442 PA0583 +
H PA14 Blast Hit H PAO1 Blast Hit