Mutant Report for Mutant ID: 31570
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_04_04
Raw Sequence ID: 16498
Chromat Path: H:\Pa14\MrT7\329v1\329v1_G10_G10_084.ab1 Last Transposon Base: 63 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTTGAATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGTCCTC
GTCGCGCAGGCGGACCGTGGAATAGTCGATGGAGTCGCCGAATACGTCTTTCGCCCATCATCTGCAGGCC
TGGCCAAGAGCACGTCCGGCCTCGGCGTCGGGGCCACTCAGGAGCTTGCCGACTGCAACCGCCGCGCCAA
CCGCCGCTGCTATCCCGGCAACTGCGGAGGCGGGCGATCTCGTGTGTTTCATATCGACCTTGGCGATGTT
CATCGCATCCTCCGGAAGCATGGGGATGGCGCCTTCGGCGTCGGTGACGCCCTCGATGACCTCGCCGTTG
GCCATGGTGATGGTATAGGGCACGTTGGGCATGGCCGTCTGCCCGTCCAGTTGCTTGAGGACGAACCGCC
GCTGCGTCTCGCCCACCTCGAACTGCGGCAACTCGACTTCCATAGCTGGTCGCGACGACACGACTGTGCT
TCTACAGCTTGACCACAAACATGCCGGGGATGCCCCATTCTAATGATGCCCCCCTTAAAAGCCACGAAGA
TCGCCCCCTACAATCACTGAAGATGCGATCATTCGTGGTTCACAACATATGCATAGCACATTCGGACTAT
ATCATTCCACTCTTTTGCTTTTTTCTTCCCTGGTTGAAGTTTATCTACTCTTGCACCTGGCGTACATAAA
AAATAACCATAGTTGGGCAGGTTCTCAGCACCTACCATCTGCCAACCCTGGGGCCAAAACAAGATCCAAA
AGCCGCGTGTCCGTGATGGTTTACCATCATCCCTCTGTGCCCCGGCTCGGTCCACCCTCCCTGGTAACTC
AGCCAGAGGCACCATGATGGTCCAGAAGAATATACAATGACGGAGAAGGAGAAAGACGAAAAGAACACTT
CTGTACGTGTTCCCCTCTCACCACTAANTATCTTGGCTCGGAAGCGTCCGACAAAAGCCTAAATGCTACT
TATATACAGGGCTTCTAT
Blast against PA14
Processed Seqquence ID: 11659
5' Trim Position: 12 3' Trim Position: 442
Processed Sequence:
(Transposon Sequence in Red)		 
ATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTAGTCCTCGTCGCGCAGGCG
GACCGTGGAATAGTCGATGGAGTCGCCGAATACGTCTTTCGCCCATCATCTGCAGGCCTGGCCAAGAGCA
CGTCCGGCCTCGGCGTCGGGGCCACTCAGGAGCTTGCCGACTGCAACCGCCGCGCCAACCGCCGCTGCTA
TCCCGGCAACTGCGGAGGCGGGCGATCTCGTGTGTTTCATATCGACCTTGGCGATGTTCATCGCATCCTC
CGGAAGCATGGGGATGGCGCCTTCGGCGTCGGTGACGCCCTCGATGACCTCGCCGTTGGCCATGGTGATG
GTATAGGGCACGTTGGGCATGGCCGTCTGCCCGTCCAGTTGCTTGAGGACGAACCGCCGCTGCGTCTCGC
CCACCTCGAA
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
52755 52755 52756 52756 52757 52757 52758 52758
PA14 BLAST Hit ID: 52755       Show Transposon Map
Identities: 289/296 (97.63%) BLAST Bit Score: 531.0 Percent of Processed Sequence That Aligns: 68.60%
Query Start: 135 Query End: 430 Subject Start: 285676 Subject End: 285971 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 285676 PA14_03220,PA14_03230* -,+
Raw Sequence Start: 285530 PA14_03220,PA14_03230* -,+
Extrapolated Insert Location: 285593 PA14_03220,PA14_03230* -,+
Default Offset Location: 285593 PA14_03220,PA14_03230* -,+
PA14 BLAST Hit ID: 52756       Show Transposon Map
Identities: 63/63 (100.00%) BLAST Bit Score: 125.0 Percent of Processed Sequence That Aligns: 14.42%
Query Start: 51 Query End: 113 Subject Start: 285592 Subject End: 285654 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 285592 PA14_03220,PA14_03230* -,+
Raw Sequence Start: 285530 PA14_03220,PA14_03230* -,+
Extrapolated Insert Location: 285593 PA14_03220,PA14_03230* -,+
Default Offset Location: 285593 PA14_03220,PA14_03230* -,+
PAO1 BLAST Hit ID: 52757       Show Transposon Map
Identities: 285/296 (96.28%) BLAST Bit Score: 500.0 Percent of Processed Sequence That Aligns: 68.60%
Query Start: 135 Query End: 430 Subject Start: 294265 Subject End: 294560 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 294265 PA0262 -
Raw Sequence Start: 294119 PA0262 -
Extrapolated Insert Location: 294182 PA0262 -
Default Offset Location: 294182 PA0262 -
PAO1 BLAST Hit ID: 52758       Show Transposon Map
Identities: 61/63 (96.82%) BLAST Bit Score: 109.0 Percent of Processed Sequence That Aligns: 14.42%
Query Start: 51 Query End: 113 Subject Start: 294181 Subject End: 294243 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 294181 PA0262 -
Raw Sequence Start: 294119 PA0262 -
Extrapolated Insert Location: 294182 PA0262 -
Default Offset Location: 294182 PA0262 -
H PA14 Blast Hit H PAO1 Blast Hit