Mutant Report for Mutant ID: 26877
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 10653
Chromat Path: H:\Pa14\MrT7\280v1\280v1_H09_H9_080.ab1 Last Transposon Base: Transposon Sequence
Match Quality:
Raw Sequence:
(Transposon Sequence in Red)		 
TGTTCAGTTCGAACGAACAGGCCTCTTCTGCTGCCGCCCGCAGAATTAATCAGGAAGCCGAAGAAGAAGG
CGAACACCGCGTCATACCCCGCAGGTCGACATCATCTGCAGGGCTGGCCACCTTGNTGGTCGCCTGGATC
GCCGAGCTGATCGTGTATTCGCTGGCCTCGCTGTTCGGCTTCGGCGAAGACCGCCGCACCCGCGAGCGTC
ACTGGAGCTGACGGCGCGACTCAGGCCGCCGCCAGTTCCAGGAACGCCCTGATCACCCGCAGGCCGCGGC
GCTTCTCCAGGCAGCCGATCAGGTGGCAGTTGGTCAGCTCGGGACCGACCAGCGGCACCGCGCGAACCCG
CGGATCGTTGCCGACCTCCAGGGAAGACACCACGCCGACGCCCAGTTCGGCGGCCACCGCTTCGGTCACC
GCTTCTCGGCTGTCCAGCTCCAGCAGCACCCGCGTCTGCCCGCCCTGGCTGGCGCAAGTGCGGTCGAAGG
TGCGGCGGGTGATCGATCCGGGTTCGCACAGCACCATGATCTGCTGGGCGAGTTCTTCCAGCGGTACGCC
TTTCTCTGCTTTCGCGCCAAGAAATGCTTGGCCTACGCAGCCATGTCCGCAGTATGCGCCGAATTCCTCC
CACGTTCCTCCAGGTAACATTCCCGGGGACGCCGCCCTCGGAACTTCGTTCAGGCTGGGCGGACGGGTCC
GAACGTGGTTCGGTGGATCAGGCGCCCCTGAGGGGTTTCCAGGGGGTTTTCCTAATCCCTCCGCTTGACG
GGGGATTCCCCCGATTGATTCGTTCTACTACCGGGGTTATCTTGTGGATTTCCTGTTTGTGGGTTATTGG
TTGCCACCTTCTAGGCGGGTGGTGATATCTTTGCTTATGGCGGCTATAGGGGTCTCGTGTTTTTCCCTTA
GGACTAAGTTGATCGTTTATCCTC
Blast against PA14
Processed Seqquence ID: 6662
5' Trim Position: 118 3' Trim Position: 425
Processed Sequence:
(Transposon Sequence in Red)		 
CACCTTGNTGGTCGCCTGGATCGCCGAGCTGATCGTGTATTCGCTGGCCTCGCTGTTCGGCTTCGGCGAA
GACCGCCGCACCCGCGAGCGTCACTGGAGCTGACGGCGCGACTCAGGCCGCCGCCAGTTCCAGGAACGCC
CTGATCACCCGCAGGCCGCGGCGCTTCTCCAGGCAGCCGATCAGGTGGCAGTTGGTCAGCTCGGGACCGA
CCAGCGGCACCGCGCGAACCCGCGGATCGTTGCCGACCTCCAGGGAAGACACCACGCCGACGCCCAGTTC
GGCGGCCACCGCTTCGGTCACCGCTTC
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
30035 30035 30036 30036
PA14 BLAST Hit ID: 30035       Show Transposon Map
Identities: 304/305 (99.67%) BLAST Bit Score: 599.0 Percent of Processed Sequence That Aligns: 99.02%
Query Start: 3 Query End: 307 Subject Start: 4215445 Subject End: 4215141 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 4215445 PA14_47320 +
Raw Sequence Start: 4215565 PA14_47320 +
Extrapolated Insert Location:
Default Offset Location: 4215502 PA14_47320 +
PAO1 BLAST Hit ID: 30036       Show Transposon Map
Identities: 300/305 (98.36%) BLAST Bit Score: 559.0 Percent of Processed Sequence That Aligns: 99.02%
Query Start: 3 Query End: 307 Subject Start: 1419189 Subject End: 1419492 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 1419189 PA1308 +
Raw Sequence Start: 1419069 PA1308 +
Extrapolated Insert Location:
Default Offset Location: 1419132 PA1308 +
H PA14 Blast Hit H PAO1 Blast Hit