Mutant Report for Mutant ID: 25957
Strain: PA14 Genetic Background: Wild Type Transposon: MrT7 Library: lib_09_03
Raw Sequence ID: 9733
Chromat Path: H:\Pa14\MrT7\271v1\271v1_D01_D1_013.ab1 Last Transposon Base: 57 Transposon Sequence
Match Quality: 0
Raw Sequence:
(Transposon Sequence in Red)		 
CGTTTGATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACTGGGGAGAAGA
GATCGCCAATGCGGCGTTCGTCCATTACCCGGACGAACGCTGGTTCAAGCCCGGCCGCGACGATGCGCTG
CCGCTGGGACTGCTCGACGGCCATTGCCTGGCGATCTACGACCCCGACGGCGAGCTGCGCGGCTCGCACC
TGTACGACACCAACTCGGGCAATGTGCAGCGCGGTATCTGCGCCTTGCCCTTCGTGCGCCAGTCCGACGG
CCAGGNNTGGTGGTNNATTTCCCCTCCAACCTGATCGAGAACCTCTACCTCAGCAACGGCATGAGCGCCG
GTAATACCCTGCCCCTCCATCATCTGCAGGCCTGGCCAANNNNNNTNNNNNNNNNNNNNNNNNNNNNNNN
NCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTGNNTNNTNNNNNNNNNNNNNNTN
NNNNNNNNNNNNNNNNNNNNNNAGGNTGGGTNTGTTTNNNNNTTGGAGGGGTAAGGAAGTGGTGGGNGNA
AGGGGGGGGGGGGTATGGGGGGGGGATTCGGGGAGTCAAGGTGGTCTCTTTATTGGGAAGGGGTAGGGGT
GTGCCCTCTCTTACTCGTCGGGGGGGCGGTGAGAATAGTTTGCTATGACGTTTACGGGTTGGAGTTGAGG
AGAGTATTATTATTGTTTCTGGGGGGGGTGGAGTTTCTAATTATTTTTTTGTGTTGATTAATTATTCTCT
TAATAAATTAATAGTCCTCAAATCTGTTATTGGTGGGTTATTC
Blast against PA14
Processed Seqquence ID: 5865
5' Trim Position: 5 3' Trim Position: 356
Processed Sequence:
(Transposon Sequence in Red)		 
GATACGACTCCTATAGGGGTCTAGAGACCGGGGACTTATCAGCCAACCTGTTACTGGGGAGAAGAGATCG
CCAATGCGGCGTTCGTCCATTACCCGGACGAACGCTGGTTCAAGCCCGGCCGCGACGATGCGCTGCCGCT
GGGACTGCTCGACGGCCATTGCCTGGCGATCTACGACCCCGACGGCGAGCTGCGCGGCTCGCACCTGTAC
GACACCAACTCGGGCAATGTGCAGCGCGGTATCTGCGCCTTGCCCTTCGTGCGCCAGTCCGACGGCCAGG
NNTGGTGGTNNATTTCCCCTCCAACCTGATCGAGAACCTCTACCTCAGCAACGGCATGAGCGCCGGTAAT
A
Blast against PA14
BLAST of Processed Sequence against PA14 And PAO1 Genome Sequence
26137 26137 26138 26138
PA14 BLAST Hit ID: 26137       Show Transposon Map
Identities: 293/300 (97.66%) BLAST Bit Score: 543.0 Percent of Processed Sequence That Aligns: 85.19%
Query Start: 52 Query End: 351 Subject Start: 3527450 Subject End: 3527156 Tn Insert Strand: -
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 3527450 PA14_39600*,PA14_39610 -,+
Raw Sequence Start: 3527506 PA14_39600*,PA14_39610 -,+
Extrapolated Insert Location: 3527449 PA14_39600*,PA14_39610 -,+
Default Offset Location: 3527443 PA14_39600*,PA14_39610 -,+
PAO1 BLAST Hit ID: 26138       Show Transposon Map
Identities: 290/300 (96.66%) BLAST Bit Score: 519.0 Percent of Processed Sequence That Aligns: 85.19%
Query Start: 52 Query End: 351 Subject Start: 2104882 Subject End: 2105176 Tn Insert Strand: +
Location Name Genome Location ORF(*=unsupported) ORF Orientation Relative to Query Sequence
Subject Start: 2104882 PA1926 +
Raw Sequence Start: 2104826 PA1926 +
Extrapolated Insert Location: 2104883 PA1926 +
Default Offset Location: 2104889 PA1926 +
H PA14 Blast Hit H PAO1 Blast Hit