ViewGene
Gene ID:
ChromosomeID:
GC_ORFID:
Genome Location:
PA4323 PA4323 PA4324 PA4324 PA4325 PA4325 4838 4838 PA4327 PA4327 PA4328 PA4328 pykA pykA 6631 6631 PA4330 PA4330 PA4331 PA4331 5453 5453 6632 6632 fumA fumA 6635 6635 6633 6633 6634 6634 6637 6637 mntH1 mntH1 6636 6636 PA4335 PA4335 PA4336 PA4336 PA4337 PA4337 6638 6638 PA4338 PA4338 PA4339 PA4339 PA4340 PA4340 PA4341 PA4341 6639 6639 PA4342 PA4342
GeneID:733
TrivialName:PA14_56300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56300
AltLocusName:PA4333
Sequence Length: 1524
Protein Length:507
Start: 5025202
Stop: 5026725
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07721.1|AE004849_8 (AE004849) probable fumarase [Pseudomonas aeruginosa PAO1]
Identities = 507/507 (100%), Positives = 507/507 (100%)
Sequence:
ATGGCCGTGATCAAGCAAGACGACCTGATCCAGAGCGTCGCCGATGCCCTGCAGTTCATCTCCTACTACCAC
CCGGTCGATTTCATCCAGGCCATGCATGAGGCCTACCTGCGCGAAGAATCGCCGGCTGCCCGCGACTCCATG
GCGCAGATCCTGATCAACTCGCGCATGTGCGCCACCGGCCACCGCCCGATCTGCCAGGACACCGGGATCGTC
ACCGTGTTCGTCCGGGTCGGCATGGACGTGCGCTGGGACGGCGCTACCATGAGCGTCGACGATATGATCAAC
GAAGGCGTGCGCCGCGCCTACAACCTGCCGGAGAACGTCCTGCGCGCCTCGATCCTGGCCGACCCGGCCGGC
GCGCGGAAGAACACCAAGGACAACACGCCGGCGGTGATCCACTACTCCATCGTTCCCGGCGACAAGGTCGAA
GTCGATGTCGCGGCCAAGGGCGGCGGCTCGGAGAACAAGTCGAAGATGGCCATGCTCAACCCGTCCGACTCG
ATCGTCGACTGGGTGCTGAAGACTGTACCGACCATGGGCGCCGGCTGGTGCCCGCCGGGCATGCTCGGCATC
GGCATCGGCGGCACCGCGGAGAAAGCAGCGGTGATGGCGAAGGAAGTCCTCATGGACCCGATCGACATCCAC
GAACTGAAGGCCCGCGGCCCGCAGAACCGCATCGAGGAACTGCGCCTGGAGCTGTTCGAGAAGGTCAACCAG
CTGGGCATCGGTGCCCAGGGCCTGGGCGGCCTGACCACCGTGCTCGACGTCAAGATCATGGACTACCCGACC
CACGCCGCCTCGCTGCCGGTGTGCATGATCCCCAACTGCGCCGCGACCCGCCACGCCCACTTCGTGCTCGAC
GGCTCCGGCCCCGCCGAACTGGAAGCGCCGTCGCTCGACGCCTACCCGGAGATCGTCTGGGAAGCCGGCCCG
TCCGCGCGCCGCGTCGACCTGGACAAGATCACCCCGGAAGAGGTGCAAAGCTGGAAACCGGGCGAGACCCTG
CTGCTCAACGGCAAGATGCTCACCGGCCGCGACGCCGCGCACAAGCGCATGGTCGACATGCTGAACAAGGGC
GAAACGCTGCCGGTGGACCTGAAAGGCCGCTTCATCTATTACGTCGGCCCGGTCGATCCGGTCGGCGACGAG
GTGGTCGGCCCGGCCGGTCCCACCACCGCCACCCGGATGGACAAGTTCACCCGCCAGATCCTCGAACAGACC
GGCCTGCTGGGCATGATCGGCAAGTCCGAGCGCGGTCCCATCGCCATCGAGGCGATCAAGGACAACAAGGCG
GTATACCTGATGGCCGTCGGCGGCGCCGCCTACCTGGTCGCCCAGGCGATCAAGAAGTCCAAGGTGCTGGCC
TTCGCCGAACTGGGCATGGAAGCGATCTACGAGTTCGAGGTGAAGGACATGCCGGTCACCGTCGCGGTCGAT
ACCAACGGCGAGTCGGTGCACATCACCGGCCCGGCGGTCTGGCAGAAGAAGATCGCCGAAAGCCTGGCGGTC
GAGGTCAAGTAA
Translation:
MAVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCATGHRPICQDTGIV
TVFVRVGMDVRWDGATMSVDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGDKVE
VDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMDPIDIH
ELKARGPQNRIEELRLELFEKVNQLGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLD
GSGPAELEAPSLDAYPEIVWEAGPSARRVDLDKITPEEVQSWKPGETLLLNGKMLTGRDAAHKRMVDMLNKG
ETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQILEQTGLLGMIGKSERGPIAIEAIKDNKA
VYLMAVGGAAYLVAQAIKKSKVLAFAELGMEAIYEFEVKDMPVTVAVDTNGESVHITGPAVWQKKIAESLAV
EVK*
AnnotationID:742GeneID:733
AnnotatorUID: diggins
Modification Date/Time: 2005-04-04 16:04:19
Gene Name:
Confidence Code:3
GeneProduct:putative fumarase
Cell Localization:()
Synonyms:fumA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:putative fumarate hydratase
Functional Category Confidence Code:5
COGs:pfam05681,pfam05683,COG1838
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66522.1|AE016281_3 (AE016777) fumarate hydratase, class I [Pseudomonas putida KT2440]
          Length = 507

 Score =  978 bits (2527), Expect = 0.0
 Identities = 486/507 (95%), Positives = 500/507 (98%)

>gb|AAO57790.1| (AE016871) fumarate hydratase, class I, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 507

 Score =  958 bits (2477), Expect = 0.0
 Identities = 475/507 (93%), Positives = 497/507 (98%)

>gb|AAM36330.1| (AE011778) fumarate hydratase [Xanthomonas axonopodis pv. citri
           str. 306]
          Length = 505

 Score =  794 bits (2050), Expect = 0.0
 Identities = 389/500 (77%), Positives = 445/500 (89%), Gaps = 1/500 (0%)








 
Structural Features:
pfam05681, Fumerase, Fumarate hydratase (Fumerase). 
CD-Length = 271 residues, 100.0% aligned
Score =  354 bits (909), Expect = 2e-98
Identities = 150/282 (53%), Positives = 186/282 (65%), Gaps = 14/282 (4%)

pfam05683, Fumerase_C, Fumarase C-terminus. 
CD-Length = 209 residues,  99.5% aligned
Score =  305 bits (782), Expect = 1e-83
Identities = 133/208 (63%), Positives = 153/208 (73%), Gaps = 4/208 (1%)

COG1838, FumA, Tartrate dehydratase beta subunit/Fumarate hydratase class I, 
C-terminal domain 
[Energy production and conversion]
CD-Length = 184 residues, 100.0% aligned
Score =  250 bits (639), Expect = 4e-67
Identities = 102/186 (54%), Positives = 135/186 (72%), Gaps = 2/186 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1469




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18611




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36688




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:733_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:733
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1279
                           PA4333_tr
                           translation of PA4333
                           507
                           99.99
                           99.99
                           0
                           2610.0
     

     
     
48338
                           gb|AAG07721.1|AE004849_8
                           (AE004849) probable fumarase [Pseudomonas aeruginosa PAO1]
                           507
                           99.99
                           99.99
                           0
                           2610.0
     

     
     
48339
                           gb|AAN66522.1|AE016281_3
                           (AE016777) fumarate hydratase, class I [Pseudomonas putida KT2440]
                           507
                           95.85
                           98.61
                           0
                           2527.0
     

     
     
48340
                           gb|AAO57790.1|
                           (AE016871) fumarate hydratase, class I, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           507
                           93.68
                           98.02
                           0
                           2477.0
     

     
     
48341
                           gb|AAM36330.1|
                           (AE011778) fumarate hydratase [Xanthomonas axonopodis pv. citri str. 306]
                           508
                           76.96
                           88.18
                           4
                           2051.0
     

     






      

          
GC ORFID: 43735How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4333Glimmer Score: 

          
Start: 4288878Stop: 4290401

          
Length: 1524


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4333 translation of PA4333Homolog Bit Score 1009.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene1953 (ORF: BLASTX 962.0), t_PA4333 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGTGATCAAGCAAGACGACCTGATCCAGAGCGTCGCCGATGCCCTGCAGTTCATCTCCTACTACCAC 
CCGGTCGATTTCATCCAGGCCATGCATGAGGCCTACCTGCGCGAAGAATCGCCGGCTGCCCGCGACTCCATG 
GCGCAGATCCTGATCAACTCGCGCATGTGCGCCACCGGCCACCGCCCGATCTGCCAGGACACCGGGATCGTC 
ACCGTGTTCGTCCGGGTCGGCATGGACGTGCGCTGGGACGGCGCTACCATGAGCGTCGACGATATGATCAAC 
GAAGGCGTGCGCCGCGCCTACAACCTGCCGGAGAACGTCCTGCGCGCCTCGATCCTGGCCGACCCGGCCGGC 
GCGCGGAAGAACACCAAGGACAACACGCCGGCGGTGATCCACTACTCCATCGTTCCCGGCGACAAGGTCGAA 
GTCGATGTCGCGGCCAAGGGCGGCGGCTCGGAGAACAAGTCGAAGATGGCCATGCTCAACCCGTCCGACTCG 
ATCGTCGACTGGGTGCTGAAGACTGTACCGACCATGGGCGCCGGCTGGTGCCCGCCGGGCATGCTCGGCATC 
GGCATCGGCGGCACCGCGGAGAAAGCAGCGGTGATGGCGAAGGAAGTCCTCATGGACCCGATCGACATCCAC 
GAACTGAAGGCCCGCGGCCCGCAGAACCGCATCGAGGAACTGCGCCTGGAGCTGTTCGAGAAGGTCAACCAG 
CTGGGCATCGGTGCCCAGGGCCTGGGCGGCCTGACCACCGTGCTCGACGTCAAGATCATGGACTACCCGACC 
CACGCCGCCTCGCTGCCGGTGTGCATGATCCCCAACTGCGCCGCGACCCGCCACGCCCACTTCGTGCTCGAC 
GGCTCCGGCCCCGCCGAACTGGAAGCGCCGTCGCTCGACGCCTACCCGGAGATCGTCTGGGAAGCCGGCCCG 
TCCGCGCGCCGCGTCGACCTGGACAAGATCACCCCGGAAGAGGTGCAAAGCTGGAAACCGGGCGAGACCCTG 
CTGCTCAACGGCAAGATGCTCACCGGCCGCGACGCCGCGCACAAGCGCATGGTCGACATGCTGAACAAGGGC 
GAAACGCTGCCGGTGGACCTGAAAGGCCGCTTCATCTATTACGTCGGCCCGGTCGATCCGGTCGGCGACGAG 
GTGGTCGGCCCGGCCGGTCCCACCACCGCCACCCGGATGGACAAGTTCACCCGCCAGATCCTCGAACAGACC 
GGCCTGCTGGGCATGATCGGCAAGTCCGAGCGCGGTCCCATCGCCATCGAGGCGATCAAGGACAACAAGGCG 
GTATACCTGATGGCCGTCGGCGGCGCCGCCTACCTGGTCGCCCAGGCGATCAAGAAGTCCAAGGTGCTGGCC 
TTCGCCGAACTGGGCATGGAAGCGATCTACGAGTTCGAGGTGAAGGACATGCCGGTCACCGTCGCGGTCGAT 
ACCAACGGCGAGTCGGTGCACATCACCGGCCCGGCGGTCTGGCAGAAGAAGATCGCCGAAAGCCTGGCGGTC 
GAGGTCAAGTAA