ViewGene
Locus Name:
GeneID:
Location:

GeneID:95
TrivialName: PA14_41560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41560
PAO1 Ortholog Locus:PA1779
Sequence Length:2727
Protein Length:908
Start: 3708903
Stop: 3711629
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05168.1|AE004603_8 (AE004603) assimilatory nitrate reductase [Pseudomonas aeruginosa PAO1]
Identities = 902/908 (99%), Positives = 905/908 (99%)
Sequence:
ATGTCGACAGATTCCCCGCTGCGCACCACCGCTTCGACCTGCTGCTACTGCGGCGTGGGTTGTGGCGTCCTG
ATCGAACACGACGGCGAACGCATCCTCGGCGTGCAAGGCGATCCGCGGCACCCGGCCAACTTCGGCAGACTC
TGCAGCAAAGGCGCCAGCCTGCACCTGACCGGCGACCTCCAGGCCCGCGCGCTGTACCCCCAACTGCGCCTC
GGCAAGCAACTGGCGCGGGCTCGCAGCGACTGGGAGAGTGCCCTGGAACATGCCGCCGGGCGTTTCGCCGAG
ACCATTCGCGAACACGGGCCGGACAGCGTGGCCTTCTATATCTCCGGCCAGTTGCTGACCGAGGACTACTAC
GCCTTCAACAAGCTGGCGCGGGCCCTGGTCGGCACCAACAACATCGACAGCAATTCGCGCCTGTGCATGTCC
TCGGCGGTGGTCGGCTACAAGCGCAGCCTGGGCGCCGACGCACCGCCGTGCAGCTACGAAGACCTCGACTGC
GCCGATTGCGTGCTGATCGCCGGCAGCAACATGGCCTTCGCCCACCCGGTCCTGTTCCGTCGCCTGGAAGCC
GCCAAGGCCGCTCGGCCGGAGATGCGCATCGTGGTGATCGACCCGCGGCGCACCGACACCTGCGAGCTGGCC
GACCTGCACCTGGCGCTGCTGCCCGGTACCGACGTCGCACTGTTCCACGGCATCCTGCATATCCTGCTCTGG
GAGGACTGGATCGACCGTTCGTTCATCGCCGAGCATACCGAGGGTTTCGCCGACCTGAAGGAACTGGTGCGC
GACTACACCCCGGCCGCCGTCGCCGACATCTGCGGCATCGACCGCGCCGACCTGCAGCGCTGCGCTGAATGG
ATCGGCCGTTCGCCGCGCTTCCTTTCGCTCTGGTGCATGGGGCTCAACCAGTCCAGCGCCGGCAGCGCCAAG
AACAGCGCACTGATCAACCTGCACCTGGCCACCGGCAAGATCGGCCGCGCCGGATGCGGACCGTTTTCCCTC
ACCGGCCAGCCGAACGCCATGGGCGGTCGCGAGACCGGCAGCCTGGCCACCCTGCTGCCCGGCCACCGCGAA
GCCGCCGATCCCGGCCATCGCGCCGAGGTGGCGCACTACTGGGGTGTGGAACAGTTGCCCACGTCCCCCGGT
CTCAGCGCCATCGAGCTGTTCGATGCGGTCCACGACGGCCGGATCAAGGCGCTCTGGATCGCCTGTACCAAC
CCCGCGCAATCGCTGCCGGACCAGCGCAAGATCCACGAGGCCCTTGCCCGCTGTCCCTTCGTGGTGGTCCAG
GAAGCCTTCGCCGGCACGGAAACCTGCCAATACGCCGACCTTTTGCTTCCAGCCGCATCCTGGGGAGAAAAG
GAAGGCTCCGTGACCAATTCGGAACGGCGGGTCAGTCATGTCCGTCGGGCCGTTCCGCCCCCCGGGGAAGCA
CGCCAGGACTGGAACATCGTCTGCGACTTCGCGCGCCGCCTGGAAGGGCACCTGCGACCCGCTAAACCCGGC
CTGTTCGCCTTTGCCGACAGCCGCAGCCTGTTCGACGAATACAAGCTGCTTACCGCCGGCCGCGACCTCGAT
CTTTCCGGCCTCAGCTACGCACTGCTCGACCGCCTGGGGCCGCAGCAATGGCCATTTCCCAGTGGCGCGGAG
CACGGCACCGCACGGCTCTATGCAGACGGCCGCTTCCCTACCGCCTCGGGACGCGCGCAACTGGTCGCCGAA
CCCTACCGCGCCGCCCAGGAAAAACGTGACGCGCGCTACCCGCTGACCCTCAATACCGGACGCTTGCGCGAC
CAGTGGCACGGCATGAGCCGGACCGGCACCTGCGCACGCCTGTTCGGCCATGAAGAGGAGGCCCTGGTGCAC
CTGCATCCCGAGGAACTGCGCCGCCGCCAACTGCGGGACGGGCAGCTCGTGCGCCTGAAGAGCCGGCGTGGC
GCCCTGATCCTGCCAGTGAGTGCAGACGACTCGGTGCGCCCCGGCCAGGCCTTCCTGCCGATGCACTGGGGC
GATCGCTTCCTCAAGGGCCTGGGCTGCAATGTCCTGACCCTGCCGGCGTTCGACCCCCTGTCGAAACAACCC
GAACTCAAGCACGCCGGGGTTCAGGTCGAATCCGTCGAACTGCCCTGGCGCCTGTTCGCGCTGGTGGAAACC
GATATCCAGGCGCGTTTCGAGGCCCTCCGTGCGCTGTGCGAAGTCTTCGACCACGCCAGCTTCAGTCTCGCC
GGACGCGAGCGCCCCGCCCTGCTGGTCAGCGCCGCACACCATGAGGCACCTGGCGCAGACCTGCTGGAGCGG
ATAGATCGCCAACTCGAATTGCTCGACGGGCCGATCCTCGCCTACGACGACCCACGGCGCGCCATCGGCAAG
CGCGTGCGCCTCGAAGACGGGCGCATCGTCGCCGTCCGGCTCGCCGGCGAAACCCTCGCCCGCGACTGGTTG
AAAGAACTCTGGCTTACCGGCCGGGCCGATAGCGAGCTACGCCGCTGGCTGCTCGCCCCACTCGGCGCGGCA
CCGGGACGACCCAGCCAGGGTGCGGGCGGCAAGACCCTGTGCAGTTGCCAGAACGTCAGCCAGCAAACGGTG
CTCGGCGGCATCGCCCGCGGATTGGACCTGGACGGATTGAAGCGCGAGTTCGGCTGCGGCACCGGCTGCGGC
TCCTGCGTACCGGAAATCAAGAGACTGCTGGCGGCCCCCCGGCCCATGGCGGCGAATGCCTGA
Translation:
MSTDSPLRTTASTCCYCGVGCGVLIEHDGERILGVQGDPRHPANFGRLCSKGASLHLTGDLQARALYPQLRL
GKQLARARSDWESALEHAAGRFAETIREHGPDSVAFYISGQLLTEDYYAFNKLARALVGTNNIDSNSRLCMS
SAVVGYKRSLGADAPPCSYEDLDCADCVLIAGSNMAFAHPVLFRRLEAAKAARPEMRIVVIDPRRTDTCELA
DLHLALLPGTDVALFHGILHILLWEDWIDRSFIAEHTEGFADLKELVRDYTPAAVADICGIDRADLQRCAEW
IGRSPRFLSLWCMGLNQSSAGSAKNSALINLHLATGKIGRAGCGPFSLTGQPNAMGGRETGSLATLLPGHRE
AADPGHRAEVAHYWGVEQLPTSPGLSAIELFDAVHDGRIKALWIACTNPAQSLPDQRKIHEALARCPFVVVQ
EAFAGTETCQYADLLLPAASWGEKEGSVTNSERRVSHVRRAVPPPGEARQDWNIVCDFARRLEGHLRPAKPG
LFAFADSRSLFDEYKLLTAGRDLDLSGLSYALLDRLGPQQWPFPSGAEHGTARLYADGRFPTASGRAQLVAE
PYRAAQEKRDARYPLTLNTGRLRDQWHGMSRTGTCARLFGHEEEALVHLHPEELRRRQLRDGQLVRLKSRRG
ALILPVSADDSVRPGQAFLPMHWGDRFLKGLGCNVLTLPAFDPLSKQPELKHAGVQVESVELPWRLFALVET
DIQARFEALRALCEVFDHASFSLAGRERPALLVSAAHHEAPGADLLERIDRQLELLDGPILAYDDPRRAIGK
RVRLEDGRIVAVRLAGETLARDWLKELWLTGRADSELRRWLLAPLGAAPGRPSQGAGGKTLCSCQNVSQQTV
LGGIARGLDLDGLKREFGCGTGCGSCVPEIKRLLAAPRPMAANA*
AnnotationID:105GeneID:95
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:32
Gene Name:
Confidence Code:2
GeneProduct:assimilatory nitrate reductase
Cell Localization:(8) Periplasmic
Synonyms:PA1779, nasA, nasC
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0243,pfam00384
Secondary Functional Category(ies):energy production and conversion
EC Number:1.7.99.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55815.1| (AE016864) nitrate reductase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 908

 Score = 1328 bits (3436), Expect = 0.0
 Identities = 641/893 (71%), Positives = 747/893 (83%), Gaps = 1/893 (0%)

>gb|AAM41298.1| (AE012305) nitrate reductase [Xanthomonas campestris pv. campestris
           str. ATCC 33913]
          Length = 912

 Score =  907 bits (2344), Expect = 0.0
 Identities = 481/912 (52%), Positives = 598/912 (65%), Gaps = 31/912 (3%)

>gb|AAK22602.1| (AE005737) NasA/NapA/NarB nitrate reductase, large subunit family
           protein [Caulobacter crescentus CB15]
          Length = 892

 Score =  677 bits (1747), Expect = 0.0
 Identities = 411/915 (44%), Positives = 523/915 (57%), Gaps = 63/915 (6%)








 
Structural Features:
COG0243, BisC, Anaerobic dehydrogenases, typically selenocysteine-containing 
[Energy production and conversion]
CD-Length = 765 residues,  95.9% aligned
Score =  508 bits (1310), Expect = 1e-144
Identities = 252/755 (33%), Positives = 349/755 (46%), Gaps = 67/755 (8%)

pfam00384, Molybdopterin, Molybdopterin oxidoreductase.
CD-Length = 359 residues, 100.0% aligned
Score =  238 bits (608), Expect = 2e-63
Identities = 124/441 (28%), Positives = 190/441 (43%), Gaps = 93/441 (21%)






   
Genomic Context:






   
Comment:


















    ReferenceID:193




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17335




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37144




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:95_cdd.html


    		




 
URL:

















    ReferenceID:37815




  
Author/Investigator(s):
        Lin JT, Goldman BS, Stewart V.





  
Title:
        Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al.




  
PubMed:
        8468296


MedLine:
        




    
Source:
J Bacteriol. 1993 Apr;175(8):2370-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8468296

















    ReferenceID:37820




  
Author/Investigator(s):
        Ogawa K, Akagawa E, Yamane K, Sun ZW, LaCelle M, Zuber P, Nakano MM.





  
Title:
        The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.




  
PubMed:
        7868621


MedLine:
        




    
Source:
J Bacteriol. 1995 Mar;177(5):1409-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7868621

















    ReferenceID:38136




  
Author/Investigator(s):
        Potter LC, Cole JA.





  
Title:
        Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12.




  
PubMed:
        10548535


MedLine:
        




    
Source:
Biochem J. 1999 Nov 15;344 Pt 1:69-76.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10548535

















    ReferenceID:38137




  
Author/Investigator(s):
        Berks BC, Richardson DJ, Reilly A, Willis AC, Ferguson SJ.





  
Title:
        The napEDABC gene cluster encoding the periplasmic nitrate reductase system of Thiosphaera pantotropha.




  
PubMed:
        7639719


MedLine:
        




    
Source:
Biochem J. 1995 Aug 1;309 ( Pt 3):983-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7639719









           
Homologs By Global Alignment 

           

       



     
Gene ID:95
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
179
                           PA1779_tr
                           translation of PA1779
                           908
                           99.33
                           99.66
                           0
                           4765.0
     

     
     
14342
                           gb|AAG05168.1|AE004603_8
                           (AE004603) assimilatory nitrate reductase [Pseudomonas aeruginosa PAO1]
                           908
                           99.33
                           99.66
                           0
                           4765.0
     

     
     
14343
                           gb|AAO55815.1|
                           (AE016864) nitrate reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           917
                           70.22
                           81.89
                           18
                           3429.0
     

     
     
14344
                           gb|AAQ59901.1|
                           (AE016917) nitrate reductase [Chromobacterium violaceum ATCC 12472]
                           923
                           53.08
                           65.22
                           36
                           2425.0
     

     
     
14345
                           gb|AAM41298.1|
                           (AE012305) nitrate reductase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           930
                           51.93
                           64.51
                           40
                           2368.0
     

     






      

          
GC ORFID: 43097How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1779Glimmer Score: 

          
Start: 1245006Stop: 1242280

          
Length: 2727


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1779 translation of PA1779Homolog Bit Score 1840.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene368 (ORF: BLASTX 1315.0), t_PA1779 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGACAGATTCCCCGCTGCGCACCACCGCTTCGACCTGCTGCTACTGCGGCGTGGGTTGTGGCGTCCTG 
ATCGAACACGACGGCGAACGCATCCTCGGCGTGCAAGGCGATCCGCGGCACCCGGCCAACTTCGGCAGACTC 
TGCAGCAAAGGCGCCAGCCTGCACCTGACCGGCGACCTCCAGGCCCGCGCGCTGTACCCCCAACTGCGCCTC 
GGCAAGCAACTGGCGCGGGCTCGCAGCGACTGGGAGAGTGCCCTGGAACATGCCGCCGGGCGTTTCGCCGAG 
ACCATTCGCGAACACGGGCCGGACAGCGTGGCCTTCTATATCTCCGGCCAGTTGCTGACCGAGGACTACTAC 
GCCTTCAACAAGCTGGCGCGGGCCCTGGTCGGCACCAACAACATCGACAGCAATTCGCGCCTGTGCATGTCC 
TCGGCGGTGGTCGGCTACAAGCGCAGCCTGGGCGCCGACGCACCGCCGTGCAGCTACGAAGACCTCGACTGC 
GCCGATTGCGTGCTGATCGCCGGCAGCAACATGGCCTTCGCCCACCCGGTCCTGTTCCGTCGCCTGGAAGCC 
GCCAAGGCCGCTCGGCCGGAGATGCGCATCGTGGTGATCGACCCGCGGCGCACCGACACCTGCGAGCTGGCC 
GACCTGCACCTGGCGCTGCTGCCCGGTACCGACGTCGCACTGTTCCACGGCATCCTGCATATCCTGCTCTGG 
GAGGACTGGATCGACCGTTCGTTCATCGCCGAGCATACCGAGGGTTTCGCCGACCTGAAGGAACTGGTGCGC 
GACTACACCCCGGCCGCCGTCGCCGACATCTGCGGCATCGACCGCGCCGACCTGCAGCGCTGCGCTGAATGG 
ATCGGCCGTTCGCCGCGCTTCCTTTCGCTCTGGTGCATGGGGCTCAACCAGTCCAGCGCCGGCAGCGCCAAG 
AACAGCGCACTGATCAACCTGCACCTGGCCACCGGCAAGATCGGCCGCGCCGGATGCGGACCGTTTTCCCTC 
ACCGGCCAGCCGAACGCCATGGGCGGTCGCGAGACCGGCAGCCTGGCCACCCTGCTGCCCGGCCACCGCGAA 
GCCGCCGATCCCGGCCATCGCGCCGAGGTGGCGCACTACTGGGGTGTGGAACAGTTGCCCACGTCCCCCGGT 
CTCAGCGCCATCGAGCTGTTCGATGCGGTCCACGACGGCCGGATCAAGGCGCTCTGGATCGCCTGTACCAAC 
CCCGCGCAATCGCTGCCGGACCAGCGCAAGATCCACGAGGCCCTTGCCCGCTGTCCCTTCGTGGTGGTCCAG 
GAAGCCTTCGCCGGCACGGAAACCTGCCAATACGCCGACCTTTTGCTTCCAGCCGCATCCTGGGGAGAAAAG 
GAAGGCTCCGTGACCAATTCGGAACGGCGGGTCAGTCATGTCCGTCGGGCCGTTCCGCCCCCCGGGGAAGCA 
CGCCAGGACTGGAACATCGTCTGCGACTTCGCGCGCCGCCTGGAAGGGCACCTGCGACCCGCTAAACCCGGC 
CTGTTCGCCTTTGCCGACAGCCGCAGCCTGTTCGACGAATACAAGCTGCTTACCGCCGGCCGCGACCTCGAT 
CTTTCCGGCCTCAGCTACGCACTGCTCGACCGCCTGGGGCCGCAGCAATGGCCATTTCCCAGTGGCGCGGAG 
CACGGCACCGCACGGCTCTATGCAGACGGCCGCTTCCCTACCGCCTCGGGACGCGCGCAACTGGTCGCCGAA 
CCCTACCGCGCCGCCCAGGAAAAACGTGACGCGCGCTACCCGCTGACCCTCAATACCGGACGCTTGCGCGAC 
CAGTGGCACGGCATGAGCCGGACCGGCACCTGCGCACGCCTGTTCGGCCATGAAGAGGAGGCCCTGGTGCAC 
CTGCATCCCGAGGAACTGCGCCGCCGCCAACTGCGGGACGGGCAGCTCGTGCGCCTGAAGAGCCGGCGTGGC 
GCCCTGATCCTGCCAGTGAGTGCAGACGACTCGGTGCGCCCCGGCCAGGCCTTCCTGCCGATGCACTGGGGC 
GATCGCTTCCTCAAGGGCCTGGGCTGCAATGTCCTGACCCTGCCGGCGTTCGACCCCCTGTCGAAACAACCC 
GAACTCAAGCACGCCGGGGTTCAGGTCGAATCCGTCGAACTGCCCTGGCGCCTGTTCGCGCTGGTGGAAACC 
GATATCCAGGCGCGTTTCGAGGCCCTCCGTGCGCTGTGCGAAGTCTTCGACCACGCCAGCTTCAGTCTCGCC 
GGACGCGAGCGCCCCGCCCTGCTGGTCAGCGCCGCACACCATGAGGCACCTGGCGCAGACCTGCTGGAGCGG 
ATAGATCGCCAACTCGAATTGCTCGACGGGCCGATCCTCGCCTACGACGACCCACGGCGCGCCATCGGCAAG 
CGCGTGCGCCTCGAAGACGGGCGCATCGTCGCCGTCCGGCTCGCCGGCGAAACCCTCGCCCGCGACTGGTTG 
AAAGAACTCTGGCTTACCGGCCGGGCCGATAGCGAGCTACGCCGCTGGCTGCTCGCCCCACTCGGCGCGGCA 
CCGGGACGACCCAGCCAGGGTGCGGGCGGCAAGACCCTGTGCAGTTGCCAGAACGTCAGCCAGCAAACGGTG 
CTCGGCGGCATCGCCCGCGGATTGGACCTGGACGGATTGAAGCGCGAGTTCGGCTGCGGCACCGGCTGCGGC 
TCCTGCGTACCGGAAATCAAGAGACTGCTGGCGGCCCCCCGGCCCATGGCGGCGAATGCCTGA