ViewGene
Locus Name:
GeneID:
Location:

GeneID:922
TrivialName: PA14_48090
AnnotatorUID: diggins
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48090
PAO1 Ortholog Locus:PA1248
Sequence Length:1446
Protein Length:481
Start: 4280768
Stop: 4279323
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04637.1|AE004554_5 (AE004554) Alkaline protease secretion outer membrane protein AprF precursor [Pseudomonas aeruginosa PAO1]
Identities = 481/481 (100%), Positives = 481/481 (100%)
Sequence:
ATGACCATGCGCCGCCTGATGACCTGGCTGTTCGGCGCctTcCTGTTGCTGCTGCGCGAGGATGCCTTCGCC
CTCGGCCTGCTGGACGGCTATCACCTGGCCCTGGAGAACGACCCGCAGTTCCAGGCCGCGATCCAGGAGCAC
GAGgCCGGCAGGCAATACCGTGCGCTCGGCCGCGCGGCGCTGCTGCCGCGCCTGGTGTACAGCTACAACCGT
GGGCGCAGTTGGTCCGATGTGACCCAGACCACCACCCGCGGCGATTTCAAGGAAGACCGCGACTACGACAGC
TACGTCTCCACCCTCAGCCTGCAACAGCCGTTGTTCGACTACGAGGCCTTCAGCCGCTACCGCAAGGGCGTG
GCCcAGGCGCTGCTGTCCGACGAGCGCTTCCGCAGCCAGAGCCAGGAACTGCTGGTCCGGGTCCTGGAAGCC
TATACCGGCGCGCTGCTGGCCCAGGACCAGATCGAACTGGCGCGGGCGCAGAAACGCTCCTACCGCGAGCAG
TTCCAGCTCAACCAGCGCCAGTTCGAGCGCGGCAACGGCACCCGCACCGACACCCTGGAGACCCAGGCCCGC
TTCAACCTGGCCCAGGCCCAGGAAATCGAGGCGCGGGACAGCCAGGATGCCGCCCTGCGCGAGCTGGAGCGA
CTGGTCGGGGCGCCGCTGGAGATCGCCGACCTGGCGCCGCTCGGCGAACGCTTCCAGGTGCGCCCGCTGAGC
CCCGCCAGCTACACCGCCTGGCGCGACCTGGCGCTGGCCGAGAATCCCGAGCTGGCCTCGCTGCGCCACGCG
GTGGACGTGGCCCGCTACGAGGTCGAGCAGAACCGCGCCGACTTCCTGCCGCGCCTGGGCCTGTACGCCAGC
ACCGGCAAGTCCAAGTCCGGTTCGGAGAACACCTACAACCAGCGCTACGAGACCGACAGCGTCGGCATCCAG
TTGAGCGTTCCGCTGTTCTCCGGCGGCGAGACCCTGGCGGCGACGCGCCAGGCCACGCATCGCATGGAGAAG
AGCCATTACGACCTGGACGACAAGGTCCGCGAGACCCTCAACCAGGTGCGCAAGATGTACAACCAGAGCAGC
AGCAGCGCGGCGAAGATCCGCGCCTACGAGATGACCGTGGATTCGGCGCGGACGTTGGTGATGGCCACGCGC
AAGAGCATCGCCGCCGGGGTGCGGGTCAACCTCGACCTGCTGAACGCCGAGCAGGCGCTGTACAGCGCGATG
AACGAGCTGTCCAAGGCCAAGTACGACTACCTCACGGCCTGGGCACGCCTGCGTTTCTACGCCGGGGTGCTG
GACGAGGCCGACCTGGAACTGGTGGCGGCGAACTTCGTTTCCGGCGAGACGCCGGCGCGCCGCCGCGACTGC
GCGACGACGGATTGCCCGGCGCCGCTGCACACCCTGTCGAAGACCGATACGGAGGAAAACCGGAGCGCGCTC
AATTGA
Translation:
MTMRRLMTWLFGAFLLLLREDAFALGLLDGYHLALENDPQFQAAIQEHEAGRQYRALGRAALLPRLVYSYNR
GRSWSDVTQTTTRGDFKEDRDYDSYVSTLSLQQPLFDYEAFSRYRKGVAQALLSDERFRSQSQELLVRVLEA
YTGALLAQDQIELARAQKRSYREQFQLNQRQFERGNGTRTDTLETQARFNLAQAQEIEARDSQDAALRELER
LVGAPLEIADLAPLGERFQVRPLSPASYTAWRDLALAENPELASLRHAVDVARYEVEQNRADFLPRLGLYAS
TGKSKSGSENTYNQRYETDSVGIQLSVPLFSGGETLAATRQATHRMEKSHYDLDDKVRETLNQVRKMYNQSS
SSAAKIRAYEMTVDSARTLVMATRKSIAAGVRVNLDLLNAEQALYSAMNELSKAKYDYLTAWARLRFYAGVL
DEADLELVAANFVSGETPARRRDCATTDCPAPLHTLSKTDTEENRSALN*
AnnotationID:931GeneID:922
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 13:01:34
Gene Name:aprF
Confidence Code:1
GeneProduct:alkaline protease secretion outer membrane protein AprF precursor
Cell Localization:(6) Outer membrane protein
Synonyms:
Cell Localization Confidence Code:1
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1538,pfam02321
Secondary Functional Category(ies):secreted factors (toxins, enzymes,alginate)
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / Intracellular trafficking and secretion]
CD-Length = 457 residues,  89.1% aligned
Score =  187 bits (475), Expect = 3e-48
Identities = 120/414 (28%), Positives = 182/414 (43%), Gaps = 9/414 (2%)

pfam02321, OEP, Outer membrane efflux protein.
CD-Length = 188 residues, 100.0% aligned
Score =  120 bits (302), Expect = 3e-28
Identities = 66/188 (35%), Positives = 101/188 (53%), Gaps = 1/188 (0%)






   
Genomic Context:
Cluster of genes including P.aeruginosa genes aprD, aprE, aprF, aprA, aprI for alkaline protease 
secretion apparatus, alkaline protease, inhibitor







   
Comment:


















    ReferenceID:1847




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18989




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37562




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:922_cdd.html


    		




 
URL:

















    ReferenceID:38039




  
Author/Investigator(s):
        Duong F, Lazdunski A, Cami B, Murgier M.





  
Title:
        Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.




  
PubMed:
        1427098


MedLine:
        




    
Source:
Gene. 1992 Nov 2;121(1):47-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1427098









           
Homologs By Global Alignment 

           

       



     
Gene ID:922
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1633
                           PA1248_tr
                           translation of PA1248
                           481
                           99.99
                           99.99
                           0
                           2425.0
     

     
     
58807
                           gb|AAG04637.1|AE004554_5
                           (AE004554) Alkaline protease secretion outer membrane protein AprF precursor [Pseudomonas aeruginosa PAO1]
                           481
                           99.99
                           99.99
                           0
                           2425.0
     

     
     
58808
                           emb|CAA45857.1|
                           (X64558) aprF [Pseudomonas aeruginosa]
                           481
                           99.99
                           99.99
                           0
                           2425.0
     

     
     
58809
                           dbj|BAA88494.1|
                           (AB023289) outer membrane protein [Pseudomonas fluorescens]
                           481
                           51.55
                           69.02
                           39
                           1259.0
     

     
     
58810
                           gb|AAF87592.1|AF286062_5
                           (AF286062) AprF [Pseudomonas brassicacearum]
                           485
                           49.89
                           67.01
                           36
                           1179.0
     

     






      

          
GC ORFID: 43924How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1248Glimmer Score: 

          
Start: 673141Stop: 674586

          
Length: 1446


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1248 translation of PA1248Homolog Bit Score 938.0

          
Other Homologs:  t_PA1248 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCATGCGCCGCCTGATGACCTGGCTGTTCGGCGCctTcCTGTTGCTGCTGCGCGAGGATGCCTTCGCC 
CTCGGCCTGCTGGACGGCTATCACCTGGCCCTGGAGAACGACCCGCAGTTCCAGGCCGCGATCCAGGAGCAC 
GAGgCCGGCAGGCAATACCGTGCGCTCGGCCGCGCGGCGCTGCTGCCGCGCCTGGTGTACAGCTACAACCGT 
GGGCGCAGTTGGTCCGATGTGACCCAGACCACCACCCGCGGCGATTTCAAGGAAGACCGCGACTACGACAGC 
TACGTCTCCACCCTCAGCCTGCAACAGCCGTTGTTCGACTACGAGGCCTTCAGCCGCTACCGCAAGGGCGTG 
GCCcAGGCGCTGCTGTCCGACGAGCGCTTCCGCAGCCAGAGCCAGGAACTGCTGGTCCGGGTCCTGGAAGCC 
TATACCGGCGCGCTGCTGGCCCAGGACCAGATCGAACTGGCGCGGGCGCAGAAACGCTCCTACCGCGAGCAG 
TTCCAGCTCAACCAGCGCCAGTTCGAGCGCGGCAACGGCACCCGCACCGACACCCTGGAGACCCAGGCCCGC 
TTCAACCTGGCCCAGGCCCAGGAAATCGAGGCGCGGGACAGCCAGGATGCCGCCCTGCGCGAGCTGGAGCGA 
CTGGTCGGGGCGCCGCTGGAGATCGCCGACCTGGCGCCGCTCGGCGAACGCTTCCAGGTGCGCCCGCTGAGC 
CCCGCCAGCTACACCGCCTGGCGCGACCTGGCGCTGGCCGAGAATCCCGAGCTGGCCTCGCTGCGCCACGCG 
GTGGACGTGGCCCGCTACGAGGTCGAGCAGAACCGCGCCGACTTCCTGCCGCGCCTGGGCCTGTACGCCAGC 
ACCGGCAAGTCCAAGTCCGGTTCGGAGAACACCTACAACCAGCGCTACGAGACCGACAGCGTCGGCATCCAG 
TTGAGCGTTCCGCTGTTCTCCGGCGGCGAGACCCTGGCGGCGACGCGCCAGGCCACGCATCGCATGGAGAAG 
AGCCATTACGACCTGGACGACAAGGTCCGCGAGACCCTCAACCAGGTGCGCAAGATGTACAACCAGAGCAGC 
AGCAGCGCGGCGAAGATCCGCGCCTACGAGATGACCGTGGATTCGGCGCGGACGTTGGTGATGGCCACGCGC 
AAGAGCATCGCCGCCGGGGTGCGGGTCAACCTCGACCTGCTGAACGCCGAGCAGGCGCTGTACAGCGCGATG 
AACGAGCTGTCCAAGGCCAAGTACGACTACCTCACGGCCTGGGCACGCCTGCGTTTCTACGCCGGGGTGCTG 
GACGAGGCCGACCTGGAACTGGTGGCGGCGAACTTCGTTTCCGGCGAGACGCCGGCGCGCCGCCGCGACTGC 
GCGACGACGGATTGCCCGGCGCCGCTGCACACCCTGTCGAAGACCGATACGGAGGAAAACCGGAGCGCGCTC 
AATTGA