ViewGene
Locus Name:
GeneID:
Location:

GeneID:85
TrivialName: PA14_68850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68850
PAO1 Ortholog Locus:PA5213
Sequence Length:2877
Protein Length:958
Start: 6143270
Stop: 6140394
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08598.1|AE004934_5 (AE004934) glycine cleavage system protein P1 [Pseudomonas aeruginosa PAO1]
Identities = 952/958 (99%), Positives = 954/958 (99%)
Sequence:
ATGTCGCAGACGCCGTCCCTCGCCCAGCTCCAGCCCGCCGATGCCTTCCTGCGCCGCCACCTCGGCCCCGAT
CCGGCGGAACAGCAGGCCATGCTGGATTTCCTCGGAGTTTCCACTCGCGCCGAACTGATCGTGCAGACGGTG
CCGCCGGCGATCCGCCTCAACCGGCCGCTGGAACTGCCCGCCGCGCTCGACGAACAGGCCGCCCTGGCGCGC
CTGCGCGGCTACGCCGGACTCAACCAGCGCTGGACCAGCCTGATCGGCATGGGCTACTACGGCACCGTGACG
CCGCCGGTGATCCTCCGCAACGTGCTGGAGAATCCCGGCTGGTACACCGCCTATACGCCCTACCAGCCGGAA
ATCGCCCAGGGCCGCCTGGAAGCGCTGCTGAATTTCCAGCAACTGACCATCGACCTCACCGGACTCGACCTG
GCCAGCGCCTCGCTGCTCGACGAAGCCACCGCCGCCGCCGAGGCCATGGCCCTGGCCAGGCGGGTGGCGAAG
GCCAGGAGCAACCGGTTCTTCGTCGATGCGCATTGCCATCCGCAGACCGTTTCGGTGTTGCGTACCCGCGCC
GAGGCGTTCGGTTTCGAGCTGGTCGTCGACGAGCCGGACAATCTTGCCGCCCACGCGGTGTTCGGCGCCTTG
CTGCAATACCCGGACAGTCGCGGCGAGATCCGCGACCTGCGCCCGCTTATCGAGGCGTTGCACGGCCAACAG
GCGCTGGCTTGCGTCGCCAGCGACCTGCTCGCCCTGTTGGTGCTGACCCCGCCGGGGGAACTCGGTGCCGAC
GTGGTGCTCGGCAGCGCCCAGCGCTTTGGCGTACCGATGGGCTACGGCGGGCCGCACGCGGCGTTCTTCGCC
ACCCGCGAAGGGTACAAGCGGGCAATGCCGGGGCGGATCATCGGCGTTTCCCGCGACGCCCGCGGCAACCCG
GCGTTGCGCATGGCCCTGCAAACCCGCGAGCAGCACATCCGTCGGGAAAAGGCCAACTCGAACATCTGCACC
GCCCAGGTGTTGCTGGCCAACATTGCTTCGCTGTATGCCGTCTACCATGGGCCGCAGGAGCTGAAACGCATC
GCCCAACGCGTCCAGCGCCTGACCGCGCTGCTGGCGGCGGGCCTGGAAAGCAAGGGACTGCGGCGGCTCAAC
CGGCACTTCTTCGACACCCTGACCTACGAGGTCGGCGAGCGTCAGGCGGCGATCCTCGAACGGGCGCGGGCG
GCACGGGTCAACCTGCGCGTGGTCGACGACCGGCGGCTGGCCCTGAGCCTCGACGAAACCTGTGACGCGGCG
ACCCTGGCGACCCTGTTCGAGATCTTCCTCGGCGCCGGCCACGGGCTGGATGTCGCGCACCTGGATGGCGGG
GCGGTGGCCGACGGCATCCCGGCGGTCCTGCAACGCACTTCGGCCTACCTGCAGCATCCGGTGTTCAACGCC
CACCACAGCGAGACCGAGATGCTTCGCTACCTGCGCCAGCTGGAAGGCAAGGACCTGGCGCTGAACCAGGCG
ATGATCCCGCTCGGCTCCTGCACCATGAAGCTCAACGCCAGCAGCGAAATGATCCCCATCACCTGGCCGGAA
TTCGCCGAACTGCATCCGTTCGTGCCGCGCGAGCAGGCCGAGGGCTATCGCCGGATGATCGACGAACTGGAA
GCCTGGCTGCGGGCGATCACCGGCTTCGACGCCATCTGCATGCAGCCGAACTCGGGAGCACAGGGCGAATAT
GCCGGGTTGCTGGCGATCCGCCGCTATCACCAGAGCCGCGGCGATAGCCAGCGCGACATCTGCCTGATCCCC
GCATCGGCGCATGGCACCAACCCGGCCTCGGCGATCATGGCCAGCATGCGGGTGGTGATCGTCGAGTGCGAT
CCCTGCGGCAACGTCGACCTCGACGACCTGCGCCTCAAGGCGGCCGAGGCGGGCGACCGGCTGTCCTGCCTG
ATGATCACCTATCCCTCGACCCACGGCGTCTACGAAGAGGGGATAGGCGAGATCTGCGAAGTGGTCCACCGC
CACGGCGGCCAGGTCTACATGGACGGCGCCAACCTCAACGCCCAGGTCGGCCTGGCGCGACCGGCGGACATC
GGCGCGGACGTCTCGCACATGAACCTACACAAGACCTTCTGCATCCCCCATGGTGGTGGCGGGCCGGGCATG
GGGCCGATCGGGGTCAAGCGGCACCTGGCGCCGTTCGTCGCCAACCATCCGGTGATCCGCGTCGAAGGGCCG
AACCCGCTGAACGACGCGGTCAGCGCGGCGCCCTGGGGCAGCGCCAGCATCCTGCCGATCAGTTGGATGTAC
ATCGCCATGATGGGCCCGCAGCTGGCCGACGCCAGCGAGGTGGCGATCCTCTCCGCCAACTACCTGGCGAAC
CGCCTGGACGGGGCGTTCCCGGTGCTCTACCGCGGGCGCAACGAGCGCGTCGCCCACGAATGCATCCTCGAC
CTGCGACCGCTCAAGGCGCAGACCGGGATAACCGAGGAAGACGTGGCCAAGCGCCTGATGGACTACgGCTTC
CACGCCCCGACCATGTCCTTCCCGGTGCCGGGCACGCTGATGGTGGAGCCGACCGAGAGCGAGTCGaagGCG
GAGCTGGATCGCTTCGTCGAGGCGATGCTGAGCATCAggGCCGAGATCGGCAAGGTGGAGAGCGGCGCCTGG
CCGGCGGAGGACAACCCGCTGAAACGGGCGCCGCATACCCTGGCCGACGTCACCGGGATCTGGCAGAGACCC
TACGAGATCGCCGAGGCAGTGACCCCGAGCGAACATGCGCGGGCGTTCAAGTACTGGCCGGCGGTTAACCGG
GTGGACAACGTCTACGGCGACCGCAACCTGTTCTGCGCCTGCGTGCCGCTGGACGACTACCGCGAGTGA
Translation:
MSQTPSLAQLQPADAFLRRHLGPDPAEQQAMLDFLGVSTRAELIVQTVPPAIRLNRPLELPAALDEQAALAR
LRGYAGLNQRWTSLIGMGYYGTVTPPVILRNVLENPGWYTAYTPYQPEIAQGRLEALLNFQQLTIDLTGLDL
ASASLLDEATAAAEAMALARRVAKARSNRFFVDAHCHPQTVSVLRTRAEAFGFELVVDEPDNLAAHAVFGAL
LQYPDSRGEIRDLRPLIEALHGQQALACVASDLLALLVLTPPGELGADVVLGSAQRFGVPMGYGGPHAAFFA
TREGYKRAMPGRIIGVSRDARGNPALRMALQTREQHIRREKANSNICTAQVLLANIASLYAVYHGPQELKRI
AQRVQRLTALLAAGLESKGLRRLNRHFFDTLTYEVGERQAAILERARAARVNLRVVDDRRLALSLDETCDAA
TLATLFEIFLGAGHGLDVAHLDGGAVADGIPAVLQRTSAYLQHPVFNAHHSETEMLRYLRQLEGKDLALNQA
MIPLGSCTMKLNASSEMIPITWPEFAELHPFVPREQAEGYRRMIDELEAWLRAITGFDAICMQPNSGAQGEY
AGLLAIRRYHQSRGDSQRDICLIPASAHGTNPASAIMASMRVVIVECDPCGNVDLDDLRLKAAEAGDRLSCL
MITYPSTHGVYEEGIGEICEVVHRHGGQVYMDGANLNAQVGLARPADIGADVSHMNLHKTFCIPHGGGGPGM
GPIGVKRHLAPFVANHPVIRVEGPNPLNDAVSAAPWGSASILPISWMYIAMMGPQLADASEVAILSANYLAN
RLDGAFPVLYRGRNERVAHECILDLRPLKAQTGITEEDVAKRLMDYGFHAPTMSFPVPGTLMVEPTESESKA
ELDRFVEAMLSIRAEIGKVESGAWPAEDNPLKRAPHTLADVTGIWQRPYEIAEAVTPSEHARAFKYWPAVNR
VDNVYGDRNLFCACVPLDDYRE*
AnnotationID:95GeneID:85
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:15
Gene Name:gcvP1
Confidence Code:2
GeneProduct:glycine cleavage system protein P1
Cell Localization:()
Synonyms:gcvP
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:glycine dehydrogenase (decarboxylating)
Functional Category Confidence Code:5
COGs:COG1003,COG0403,pfam02347
Secondary Functional Category(ies):central intermediary metabolism
EC Number:1.4.4.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70757.1|AE016719_8 (AE016793) glycine cleavage system P protein [Pseudomonas putida
           KT2440]
          Length = 957

 Score = 1542 bits (3993), Expect = 0.0
 Identities = 759/957 (79%), Positives = 839/957 (87%)

>gb|AAN81931.1|AE016766_19 (AE016766) Glycine dehydrogenase [decarboxylating] [Escherichia
           coli CFT073]
          Length = 957

 Score = 1257 bits (3252), Expect = 0.0
 Identities = 629/959 (65%), Positives = 754/959 (78%), Gaps = 8/959 (0%)

>gb|AAO70523.1| (AE016844) glycine dehydrogenase [Salmonella enterica subsp.
           enterica serovar Typhi Ty2]
          Length = 957

 Score = 1255 bits (3248), Expect = 0.0
 Identities = 629/959 (65%), Positives = 752/959 (78%), Gaps = 8/959 (0%)






 
Structural Features:
COG1003, GcvP, Glycine cleavage system protein P (pyridoxal-binding), 
C-terminal domain 
[Amino acid transport and metabolism]
CD-Length = 496 residues,  99.4% aligned
Score =  721 bits (1862), Expect = 0.0
Identities = 299/499 (59%), Positives = 348/499 (69%), Gaps = 33/499 (6%)

COG0403, GcvP, Glycine cleavage system protein P (pyridoxal-binding), 
N-terminal domain 
[Amino acid transport and metabolism]
CD-Length = 450 residues, 100.0% aligned
Score =  619 bits (1599), Expect = 3e-178
Identities = 257/450 (57%), Positives = 308/450 (68%), Gaps = 12/450 (2%)

pfam02347, GDC-P, Glycine cleavage system P-protein.
CD-Length = 430 residues, 100.0% aligned
Score =  563 bits (1452), Expect = 4e-161
Identities = 249/430 (57%), Positives = 305/430 (70%), Gaps = 6/430 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:173




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17315




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34846




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:85_cdd.html


    		




 
URL:

















    ReferenceID:35758




  
Author/Investigator(s):
        Eur J Biochem. 1993 Sep 1;216(2):539-48.





  
Title:
        Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system.




  
PubMed:
        8375392


MedLine:
        




    
Source:
Eur J Biochem. 1993 Sep 1;216(2):539-48.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8375392

















    ReferenceID:35760




  
Author/Investigator(s):
        Stauffer LT, Fogarty SJ, Stauffer GV.





  
Title:
        Characterization of the Escherichia coli gcv operon.




  
PubMed:
        8181752


MedLine:
        




    
Source:
Gene. 1994 May 3;142(1):17-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8181752









           
Homologs By Global Alignment 

           

       



     
Gene ID:85
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
163
                           PA5213_tr
                           translation of PA5213
                           958
                           99.37
                           99.58
                           0
                           4927.0
     

     
     
14002
                           gb|AAG08598.1|AE004934_5
                           (AE004934) glycine cleavage system protein P1 [Pseudomonas aeruginosa PAO1]
                           958
                           99.37
                           99.58
                           0
                           4927.0
     

     
     
14003
                           gb|AAN70757.1|AE016719_8
                           (AE016793) glycine cleavage system P protein [Pseudomonas putida KT2440]
                           958
                           79.22
                           87.57
                           1
                           3993.0
     

     
     
14005
                           emb|CAA52146.1|
                           (X73958) glycine dehydrogenase (decarboxylating) [Escherichia coli]
                           964
                           65.66
                           78.52
                           13
                           3264.0
     

     
     
14006
                           gb|AAC75941.1|
                           (AE000373) glycine decarboxylase, P protein of glycine cleavage system [Escherichia coli K12]
                           964
                           65.66
                           78.52
                           13
                           3264.0
     

     






      

          
GC ORFID: 43087How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5213Glimmer Score: 

          
Start: 5406956Stop: 5404080

          
Length: 2877


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5213 translation of PA5213Homolog Bit Score 1902.0

          
Other Homologs:  t_PA2445 (ORF: BLASTX 1166.0), psyr_15may02_Scaffold3_revised_gene4966 (ORF: BLASTX 1157.0), t_PA5213 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGCAGACGCCGTCCCTCGCCCAGCTCCAGCCCGCCGATGCCTTCCTGCGCCGCCACCTCGGCCCCGAT 
CCGGCGGAACAGCAGGCCATGCTGGATTTCCTCGGAGTTTCCACTCGCGCCGAACTGATCGTGCAGACGGTG 
CCGCCGGCGATCCGCCTCAACCGGCCGCTGGAACTGCCCGCCGCGCTCGACGAACAGGCCGCCCTGGCGCGC 
CTGCGCGGCTACGCCGGACTCAACCAGCGCTGGACCAGCCTGATCGGCATGGGCTACTACGGCACCGTGACG 
CCGCCGGTGATCCTCCGCAACGTGCTGGAGAATCCCGGCTGGTACACCGCCTATACGCCCTACCAGCCGGAA 
ATCGCCCAGGGCCGCCTGGAAGCGCTGCTGAATTTCCAGCAACTGACCATCGACCTCACCGGACTCGACCTG 
GCCAGCGCCTCGCTGCTCGACGAAGCCACCGCCGCCGCCGAGGCCATGGCCCTGGCCAGGCGGGTGGCGAAG 
GCCAGGAGCAACCGGTTCTTCGTCGATGCGCATTGCCATCCGCAGACCGTTTCGGTGTTGCGTACCCGCGCC 
GAGGCGTTCGGTTTCGAGCTGGTCGTCGACGAGCCGGACAATCTTGCCGCCCACGCGGTGTTCGGCGCCTTG 
CTGCAATACCCGGACAGTCGCGGCGAGATCCGCGACCTGCGCCCGCTTATCGAGGCGTTGCACGGCCAACAG 
GCGCTGGCTTGCGTCGCCAGCGACCTGCTCGCCCTGTTGGTGCTGACCCCGCCGGGGGAACTCGGTGCCGAC 
GTGGTGCTCGGCAGCGCCCAGCGCTTTGGCGTACCGATGGGCTACGGCGGGCCGCACGCGGCGTTCTTCGCC 
ACCCGCGAAGGGTACAAGCGGGCAATGCCGGGGCGGATCATCGGCGTTTCCCGCGACGCCCGCGGCAACCCG 
GCGTTGCGCATGGCCCTGCAAACCCGCGAGCAGCACATCCGTCGGGAAAAGGCCAACTCGAACATCTGCACC 
GCCCAGGTGTTGCTGGCCAACATTGCTTCGCTGTATGCCGTCTACCATGGGCCGCAGGAGCTGAAACGCATC 
GCCCAACGCGTCCAGCGCCTGACCGCGCTGCTGGCGGCGGGCCTGGAAAGCAAGGGACTGCGGCGGCTCAAC 
CGGCACTTCTTCGACACCCTGACCTACGAGGTCGGCGAGCGTCAGGCGGCGATCCTCGAACGGGCGCGGGCG 
GCACGGGTCAACCTGCGCGTGGTCGACGACCGGCGGCTGGCCCTGAGCCTCGACGAAACCTGTGACGCGGCG 
ACCCTGGCGACCCTGTTCGAGATCTTCCTCGGCGCCGGCCACGGGCTGGATGTCGCGCACCTGGATGGCGGG 
GCGGTGGCCGACGGCATCCCGGCGGTCCTGCAACGCACTTCGGCCTACCTGCAGCATCCGGTGTTCAACGCC 
CACCACAGCGAGACCGAGATGCTTCGCTACCTGCGCCAGCTGGAAGGCAAGGACCTGGCGCTGAACCAGGCG 
ATGATCCCGCTCGGCTCCTGCACCATGAAGCTCAACGCCAGCAGCGAAATGATCCCCATCACCTGGCCGGAA 
TTCGCCGAACTGCATCCGTTCGTGCCGCGCGAGCAGGCCGAGGGCTATCGCCGGATGATCGACGAACTGGAA 
GCCTGGCTGCGGGCGATCACCGGCTTCGACGCCATCTGCATGCAGCCGAACTCGGGAGCACAGGGCGAATAT 
GCCGGGTTGCTGGCGATCCGCCGCTATCACCAGAGCCGCGGCGATAGCCAGCGCGACATCTGCCTGATCCCC 
GCATCGGCGCATGGCACCAACCCGGCCTCGGCGATCATGGCCAGCATGCGGGTGGTGATCGTCGAGTGCGAT 
CCCTGCGGCAACGTCGACCTCGACGACCTGCGCCTCAAGGCGGCCGAGGCGGGCGACCGGCTGTCCTGCCTG 
ATGATCACCTATCCCTCGACCCACGGCGTCTACGAAGAGGGGATAGGCGAGATCTGCGAAGTGGTCCACCGC 
CACGGCGGCCAGGTCTACATGGACGGCGCCAACCTCAACGCCCAGGTCGGCCTGGCGCGACCGGCGGACATC 
GGCGCGGACGTCTCGCACATGAACCTACACAAGACCTTCTGCATCCCCCATGGTGGTGGCGGGCCGGGCATG 
GGGCCGATCGGGGTCAAGCGGCACCTGGCGCCGTTCGTCGCCAACCATCCGGTGATCCGCGTCGAAGGGCCG 
AACCCGCTGAACGACGCGGTCAGCGCGGCGCCCTGGGGCAGCGCCAGCATCCTGCCGATCAGTTGGATGTAC 
ATCGCCATGATGGGCCCGCAGCTGGCCGACGCCAGCGAGGTGGCGATCCTCTCCGCCAACTACCTGGCGAAC 
CGCCTGGACGGGGCGTTCCCGGTGCTCTACCGCGGGCGCAACGAGCGCGTCGCCCACGAATGCATCCTCGAC 
CTGCGACCGCTCAAGGCGCAGACCGGGATAACCGAGGAAGACGTGGCCAAGCGCCTGATGGACTACgGCTTC 
CACGCCCCGACCATGTCCTTCCCGGTGCCGGGCACGCTGATGGTGGAGCCGACCGAGAGCGAGTCGaagGCG 
GAGCTGGATCGCTTCGTCGAGGCGATGCTGAGCATCAggGCCGAGATCGGCAAGGTGGAGAGCGGCGCCTGG 
CCGGCGGAGGACAACCCGCTGAAACGGGCGCCGCATACCCTGGCCGACGTCACCGGGATCTGGCAGAGACCC 
TACGAGATCGCCGAGGCAGTGACCCCGAGCGAACATGCGCGGGCGTTCAAGTACTGGCCGGCGGTTAACCGG 
GTGGACAACGTCTACGGCGACCGCAACCTGTTCTGCGCCTGCGTGCCGCTGGACGACTACCGCGAGTGA