ViewGene
Locus Name:
GeneID:
Location:

GeneID:6909
TrivialName: PA14_69790
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69790
PAO1 Ortholog Locus:
Sequence Length:1548
Protein Length:515
Start: 6225657
Stop: 6224111
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/02/06. PA14_69790 GeneID 6909) and PA14_69800 (GeneID 6908) replaced by PA14_69795 (GeneID 7384). Homology: gb|AAG08672.1|AE004941_5 (AE004941) ammonium transporter AmtB [Pseudomonas aeruginosa PAO1]
Identities = 266/266 (100%), Positives = 266/266 (100%)
Sequence:
ATGACTCTGCGTAAGATCGCAGGGCTAGGAGCCCTATTGTCCCTCGCTTTGCCGGGCGTCGCCCTGGCCGCT
GAGGAACCCGTCCTGAACAGCGGCGACACCGCCTGGATGCTGATCTCCACTGCGCTCGTCCTGCTCATGACC
ATCCCCGGTCTGGCGCTGTTCTACGGCGGCATGGTGCGAGCGAAGAACGTGCTGTCGATCATGATGCAGTGC
TTCGCCATCACCGGCCTGATCACCATTCTCTGGGTGGTCTATGGCTACAGCCTGGCGTTCGATACCGCCGGG
ATGGAGAAGGGCGTCCTCAACTTCAATTCCTTCGTCGGCGGGCTGGACAAGGCCTTCCTCAGCGGTCTCACC
GCCGACGGCCTGACTTCCGCCACCGCGCTGTTCCCGGAAAGCGTGTTCATCACCTTCCAGATGACCTTCGCG
ATCATCACACCGGCGCTGATCGTCGGCGCCTTCGCCGAGCGCATGAAGTTCTCGGCGATGCTGATCTTCATG
GCGGTCTGGTTCACCGTGGTCTACGCGCCGATCGCGCACATGGTCTGGAGCGGCGACGGCGCCCTGATGTGG
GACTGGGGCGTGCTGGACTTCGCCGGCGGCACCGTGGTGCACATCAACGCCGGTATCGCCGGCCTGGTCGCC
TGCCTGGTGCTGGGCAAGCGCAAGGGCTACCCGACCACGCCGATGGCGCCGCACAACCTCGGCTACACCCTG
GTCGGGGCGGCCATGCTGTGGATCGGCTGGTTCGGTTTCAACGCCGGTTCCGCCGCCGCCGCCAACGGCACC
GCCGGGGATGGCCATGCTGGTGACCCAGATCGCTACCGCCGCCGCGGCCCTGGCCTGGATGTTCGCCGAGTG
GATCACCCACGGCAAGCCGAGCGCCCTGGGCATCGCGTCCGGCGTGGTCGCCGGTCTGGTGGCGATCACCCC
GGCCGCCGGCACCGCCGGTCCGATGGGCGCGCTGGTGATCGGCCTGGCCTCCGGGGTGATCTGCTTCTTCGC
CGCCACCAGTCTCAAGCGCGCGCTGAAATACGACGATTCCCTCGACGCCTTCGGCGTACATGCGGTCGGCGG
TATCGTCGGCGCGCTGCTCACCGGGATCTTCGCGGCGCCGTCGCTGGGCGGCTTCGGCAGCGTGGAGGACAT
CGGCGCGCAGTTCTTCGTGCAGTTCAAGGGCGTGGCCTTCACCGTGGTCTACACCGCCGTGGTGACCTTCGT
GATCCTCAAGGTGCTGGACCTGGTCATGGGCCTGCGGGTCACCGAGGAAGAGGAAGCGGTCGGCCTCGACCT
GGCGCTGCACAACGAGCGCGGCTACAACCTGTAATACCTGCCGGCGCCCGGCTTTCCGCCGGGCGTCGGTTT
GCCTCCCGCGCTCGCCTTCGCCGAACGCCATGCCCTTGCGCGTCGAAAGTCCTGGGCGTTTCCCGCGACTGC
CACGACCTCTCCGCAACCGGTCTTTCAGCGACTGCCGGCGGACTGCGCAACCCTCAGCTTTCACAGGTCTGT
CTCTTACGCTCTTTGCTTTTTCAAATGCCGCGCTAG
Translation:
MTLRKIAGLGALLSLALPGVALAAEEPVLNSGDTAWMLISTALVLLMTIPGLALFYGGMVRAKNVLSIMMQC
FAITGLITILWVVYGYSLAFDTAGMEKGVLNFNSFVGGLDKAFLSGLTADGLTSATALFPESVFITFQMTFA
IITPALIVGAFAERMKFSAMLIFMAVWFTVVYAPIAHMVWSGDGALMWDWGVLDFAGGTVVHINAGIAGLVA
CLVLGKRKGYPTTPMAPHNLGYTLVGAAMLWIGWFGFNAGSAAAANGTAGDGHAGDPDRYRRRGPGLDVRRV
DHPRQAERPGHRVRRGRRSGGDHPGRRHRRSDGRAGDRPGLRGDLLLRRHQSQARAEIRRFPRRLRRTCGRR
YRRRAAHRDLRGAVAGRLRQRGGHRRAVLRAVQGRGLHRGLHRRGDLRDPQGAGPGHGPAGHRGRGSGRPRP
GAAQRARLQPVIPAGARLSAGRRFASRARLRRTPCPCASKVLGVSRDCHDLSATGLSATAGGLRNPQLSQVC
LLRSLLFQMPR*
AnnotationID:6910GeneID:6909
AnnotatorUID: danlee
Modification Date/Time: 2006-02-02 12:12:55
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70798.1|AE016723_10 (AE016793) ammonium transporter [Pseudomonas putida KT2440]
          Length = 443

 Score =  491 bits (1263), Expect = e-138
 Identities = 240/267 (89%), Positives = 254/267 (95%), Gaps = 1/267 (0%)

>gb|AAO53764.1| (AE016856) ammonium transporter [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 445

 Score =  471 bits (1213), Expect = e-132
 Identities = 227/269 (84%), Positives = 249/269 (92%), Gaps = 3/269 (1%)

>gb|AAL83554.1|AF414717_4 (AF414717) AmtB1 [Pseudomonas stutzeri]
          Length = 438

 Score =  459 bits (1182), Expect = e-128
 Identities = 227/266 (85%), Positives = 243/266 (91%), Gaps = 4/266 (1%)








 
Structural Features:
pfam00909, Ammonium_transp, Ammonium Transporter Family.
CD-Length = 395 residues, only  55.2% aligned
Score =  218 bits (557), Expect = 1e-57
Identities = 107/234 (45%), Positives = 138/234 (58%), Gaps = 18/234 (7%)







   
Genomic Context:






   
Comment:
Inactivated 2/02/06.  PA14_69790 GeneID 6909) and PA14_69800 (GeneID 6908) 
replaced by PA14_69795 (GeneID 7384).


















    ReferenceID:13514




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30897




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34812




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6909_cdd.html


    		




 
URL:

















    ReferenceID:35717




  
Author/Investigator(s):
        Vermeiren H, Keijers V, Vanderleyden J.





  
Title:
        Isolation and sequence analysis of the GlnKamtB1amtB2 gene cluster, encoding a PII homologue and two putative ammonium transporters, from Pseudomonas stutzeri A15.




  
PubMed:
        12180140


MedLine:
        




    
Source:
DNA Seq. 2002 Feb;13(1):67-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12180140









           
Homologs By Global Alignment 

           

       



     
Gene ID:6909
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8050
                           PA5287_tr
                           translation of PA5287
                           587
                           51.10
                           54.17
                           217
                           1311.0
     

     
     
202875
                           gb|AAG08672.1|AE004941_5
                           (AE004941) ammonium transporter AmtB [Pseudomonas aeruginosa PAO1]
                           587
                           51.10
                           54.17
                           217
                           1311.0
     

     
     
202876
                           gb|AAN70798.1|AE016723_10
                           (AE016793) ammonium transporter [Pseudomonas putida KT2440]
                           563
                           47.95
                           53.46
                           168
                           1209.0
     

     
     
202878
                           gb|AAL83554.1|AF414717_4
                           (AF414717) AmtB1 [Pseudomonas stutzeri]
                           585
                           44.10
                           49.57
                           217
                           1132.0
     

     
     
202877
                           gb|AAO53764.1|
                           (AE016856) ammonium transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           588
                           44.04
                           49.65
                           216
                           1154.0
     

     






      

          
GC ORFID: 49911How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5489345Stop: 5487798

          
Length: 1548


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5287 translation of PA5287Homolog Bit Score 531.0

          
Other Homologs:  t_PA5287 translation of PA5287 (BLASTP 531), psyr_15may02_Scaffold2_revised_gene3960 related to: gi|15600480|ref|NP_253974.1| (NC_002516) ammonium transporter AmtB [Pseudomonas aeruginosa] pir||C82985 ammonium transporter AmtB PA5287 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG08672.1|AE004941_5 (AE004941) ammonium transporter Am-TRUNCATED- (BLASTP 471), psyr_15may02_Scaffold1_revised_gene1240 related to: gi|19033136|gb|AAL83554.1|AF414717_4 (AF414717) AmtB1 [Pseudomonas stutzeri] (BLASTP 361)

          
GC ORF Sequence

           
ATGACTCTGCGTAAGATCGCAGGGCTAGGAGCCCTATTGTCCCTCGCTTTGCCGGGCGTCGCCCTGGCCGCT 
GAGGAACCCGTCCTGAACAGCGGCGACACCGCCTGGATGCTGATCTCCACTGCGCTCGTCCTGCTCATGACC 
ATCCCCGGTCTGGCGCTGTTCTACGGCGGCATGGTGCGAGCGAAGAACGTGCTGTCGATCATGATGCAGTGC 
TTCGCCATCACCGGCCTGATCACCATTCTCTGGGTGGTCTATGGCTACAGCCTGGCGTTCGATACCGCCGGG 
ATGGAGAAGGGCGTCCTCAACTTCAATTCCTTCGTCGGCGGGCTGGACAAGGCCTTCCTCAGCGGTCTCACC 
GCCGACGGCCTGACTTCCGCCACCGCGCTGTTCCCGGAAAGCGTGTTCATCACCTTCCAGATGACCTTCGCG 
ATCATCACACCGGCGCTGATCGTCGGCGCCTTCGCCGAGCGCATGAAGTTCTCGGCGATGCTGATCTTCATG 
GCGGTCTGGTTCACCGTGGTCTACGCGCCGATCGCGCACATGGTCTGGAGCGGCGACGGCGCCCTGATGTGG 
GACTGGGGCGTGCTGGACTTCGCCGGCGGCACCGTGGTGCACATCAACGCCGGTATCGCCGGCCTGGTCGCC 
TGCCTGGTGCTGGGCAAGCGCAAGGGCTACCCGACCACGCCGATGGCGCCGCACAACCTCGGCTACACCCTG 
GTCGGGGCGGCCATGCTGTGGATCGGCTGGTTCGGTTTCAACGCCGGTTCCGCCGCCGCCGCCAACGGCACC 
GCCGGGGATGGCCATGCTGGTGACCCAGATCGCTACCGCCGCCGCGGCCCTGGCCTGGATGTTCGCCGAGTG 
GATCACCCACGGCAAGCCGAGCGCCCTGGGCATCGCGTCCGGCGTGGTCGCCGGTCTGGTGGCGATCACCCC 
GGCCGCCGGCACCGCCGGTCCGATGGGCGCGCTGGTGATCGGCCTGGCCTCCGGGGTGATCTGCTTCTTCGC 
CGCCACCAGTCTCAAGCGCGCGCTGAAATACGACGATTCCCTCGACGCCTTCGGCGTACATGCGGTCGGCGG 
TATCGTCGGCGCGCTGCTCACCGGGATCTTCGCGGCGCCGTCGCTGGGCGGCTTCGGCAGCGTGGAGGACAT 
CGGCGCGCAGTTCTTCGTGCAGTTCAAGGGCGTGGCCTTCACCGTGGTCTACACCGCCGTGGTGACCTTCGT 
GATCCTCAAGGTGCTGGACCTGGTCATGGGCCTGCGGGTCACCGAGGAAGAGGAAGCGGTCGGCCTCGACCT 
GGCGCTGCACAACGAGCGCGGCTACAACCTGTAATACCTGCCGGCGCCCGGCTTTCCGCCGGGCGTCGGTTT 
GCCTCCCGCGCTCGCCTTCGCCGAACGCCATGCCCTTGCGCGTCGAAAGTCCTGGGCGTTTCCCGCGACTGC 
CACGACCTCTCCGCAACCGGTCTTTCAGCGACTGCCGGCGGACTGCGCAACCCTCAGCTTTCACAGGTCTGT 
CTCTTACGCTCTTTGCTTTTTCAAATGCCGCGCTAG