ViewGene
Locus Name:
GeneID:
Location:

GeneID:6908
TrivialName: PA14_69800
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69800
PAO1 Ortholog Locus:
Sequence Length:525
Protein Length:174
Start: 6224853
Stop: 6224329
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Inactivated 2/02/06. PA14_69790 GeneID 6909) and PA14_69800 (GeneID 6908) replaced by PA14_69795 (GeneID 7384). Homology: gb|AAG08672.1|AE004941_5 (AE004941) ammonium transporter AmtB [Pseudomonas aeruginosa PAO1]
Identities = 174/174 (100%), Positives = 174/174 (100%)
Sequence:
ATGCTGGTGACCCAGATCGCTACCGCCGCCGCGGCCCTGGCCTGGATGTTCGCCGAGTGGATCACCCACGGC
AAGCCGAGCGCCCTGGGCATCGCGTCCGGCGTGGTCGCCGGTCTGGTGGCGATCACCCCGGCCGCCGGCACC
GCCGGTCCGATGGGCGCGCTGGTGATCGGCCTGGCCTCCGGGGTGATCTGCTTCTTCGCCGCCACCAGTCTC
AAGCGCGCGCTGAAATACGACGATTCCCTCGACGCCTTCGGCGTACATGCGGTCGGCGGTATCGTCGGCGCG
CTGCTCACCGGGATCTTCGCGGCGCCGTCGCTGGGCGGCTTCGGCAGCGTGGAGGACATCGGCGCGCAGTTC
TTCGTGCAGTTCAAGGGCGTGGCCTTCACCGTGGTCTACACCGCCGTGGTGACCTTCGTGATCCTCAAGGTG
CTGGACCTGGTCATGGGCCTGCGGGTCACCGAGGAAGAGGAAGCGGTCGGCCTCGACCTGGCGCTGCACAAC
GAGCGCGGCTACAACCTGTAA
Translation:
MLVTQIATAAAALAWMFAEWITHGKPSALGIASGVVAGLVAITPAAGTAGPMGALVIGLASGVICFFAATSL
KRALKYDDSLDAFGVHAVGGIVGALLTGIFAAPSLGGFGSVEDIGAQFFVQFKGVAFTVVYTAVVTFVILKV
LDLVMGLRVTEEEEAVGLDLALHNERGYNL*
AnnotationID:6909GeneID:6908
AnnotatorUID: danlee
Modification Date/Time: 2006-02-02 12:12:58
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53764.1| (AE016856) ammonium transporter [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 445

 Score =  305 bits (781), Expect = 8e-83
 Identities = 150/174 (86%), Positives = 164/174 (94%)

>gb|AAL83554.1|AF414717_4 (AF414717) AmtB1 [Pseudomonas stutzeri]
          Length = 438

 Score =  294 bits (753), Expect = 1e-79
 Identities = 145/174 (83%), Positives = 157/174 (90%)

>gb|AAN70798.1|AE016723_10 (AE016793) ammonium transporter [Pseudomonas putida KT2440]
          Length = 443

 Score =  290 bits (742), Expect = 3e-78
 Identities = 142/174 (81%), Positives = 159/174 (91%)






 
Structural Features:
pfam00909, Ammonium_transp, Ammonium Transporter Family.
CD-Length = 395 residues, only  44.3% aligned
Score =  135 bits (341), Expect = 3e-33
Identities = 80/176 (45%), Positives = 101/176 (57%), Gaps = 5/176 (2%)






   
Genomic Context:






   
Comment:
Inactivated 2/02/06.  PA14_69790 GeneID 6909) and PA14_69800 (GeneID 6908) 
replaced by PA14_69795 (GeneID 7384).


















    ReferenceID:13512




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30895




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34811




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6908_cdd.html


    		




 
URL:

















    ReferenceID:35718




  
Author/Investigator(s):
        Vermeiren H, Keijers V, Vanderleyden J.





  
Title:
        Isolation and sequence analysis of the GlnKamtB1amtB2 gene cluster, encoding a PII homologue and two putative ammonium transporters, from Pseudomonas stutzeri A15.




  
PubMed:
        12180140


MedLine:
        




    
Source:
DNA Seq. 2002 Feb;13(1):67-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12180140









           
Homologs By Global Alignment 

           

       



     
Gene ID:6908
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8049
                           PA5287_tr
                           translation of PA5287
                           442
                           39.36
                           39.36
                           268
                           863.0
     

     
     
202787
                           gb|AAG08672.1|AE004941_5
                           (AE004941) ammonium transporter AmtB [Pseudomonas aeruginosa PAO1]
                           442
                           39.36
                           39.36
                           268
                           863.0
     

     
     
202857
                           gb|AAF04767.1|AF104224_1
                           (AF104224) probable ammonium transporter [Listeria monocytogenes]
                           211
                           36.96
                           50.23
                           43
                           368.0
     

     
     
202841
                           dbj|BAC45873.1|
                           (AP005937) blr0608 [Bradyrhizobium japonicum USDA 110]
                           225
                           35.11
                           48.00
                           53
                           398.0
     

     
     
202788
                           gb|AAO53764.1|
                           (AE016856) ammonium transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           445
                           33.70
                           36.85
                           271
                           781.0
     

     






      

          
GC ORFID: 49910How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5488540Stop: 5488016

          
Length: 525


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5287 translation of PA5287Homolog Bit Score 337.0

          
Other Homologs:  t_PA5287 translation of PA5287 (BLASTP 337), psyr_15may02_Scaffold2_revised_gene3960 related to: gi|15600480|ref|NP_253974.1| (NC_002516) ammonium transporter AmtB [Pseudomonas aeruginosa] pir||C82985 ammonium transporter AmtB PA5287 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG08672.1|AE004941_5 (AE004941) ammonium transporter Am-TRUNCATED- (BLASTP 305), psyr_15may02_Scaffold1_revised_gene1240 related to: gi|19033136|gb|AAL83554.1|AF414717_4 (AF414717) AmtB1 [Pseudomonas stutzeri] (BLASTP 192)

          
GC ORF Sequence

           
ATGCTGGTGACCCAGATCGCTACCGCCGCCGCGGCCCTGGCCTGGATGTTCGCCGAGTGGATCACCCACGGC 
AAGCCGAGCGCCCTGGGCATCGCGTCCGGCGTGGTCGCCGGTCTGGTGGCGATCACCCCGGCCGCCGGCACC 
GCCGGTCCGATGGGCGCGCTGGTGATCGGCCTGGCCTCCGGGGTGATCTGCTTCTTCGCCGCCACCAGTCTC 
AAGCGCGCGCTGAAATACGACGATTCCCTCGACGCCTTCGGCGTACATGCGGTCGGCGGTATCGTCGGCGCG 
CTGCTCACCGGGATCTTCGCGGCGCCGTCGCTGGGCGGCTTCGGCAGCGTGGAGGACATCGGCGCGCAGTTC 
TTCGTGCAGTTCAAGGGCGTGGCCTTCACCGTGGTCTACACCGCCGTGGTGACCTTCGTGATCCTCAAGGTG 
CTGGACCTGGTCATGGGCCTGCGGGTCACCGAGGAAGAGGAAGCGGTCGGCCTCGACCTGGCGCTGCACAAC 
GAGCGCGGCTACAACCTGTAA