ViewGene
Locus Name:
GeneID:
Location:

GeneID:6848
TrivialName: PA14_67070
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67070
PAO1 Ortholog Locus:
Sequence Length:2148
Protein Length:715
Start: 5988239
Stop: 5986093
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/01/06. PA14_67070 (GeneID 6848) and PA14_67060 (GeneID 6847) replaced by PA14_67065 (GeneID 7383). Homology: gb|AAG08462.1|AE004920_12 (AE004920) periplasmic glucans biosynthesis protein MdoH [Pseudomonas aeruginosa PAO1]
Identities = 436/442 (98%), Positives = 438/442 (99%)
Sequence:
ATGAATAACCCATCCACTACGAAAGCACCGCTGGCCGACTACCTCGCTCATCTTCCCCTGGCGGAAGAGGAG
CGGGAGCGGCTTGGCGAGTCCGCTTCCTTCTCCGAGCTGCACGCTCGCCTGGCGGGAGCGGAAGGCGCCGCT
GCCGATGCCGGGGGCGATCCCGCCCTGGCCTCGGTACGCGCCCGCCTGCAGCTGGGCACCCCTGAGCTGGAC
GACGCCGAGATGTTCGGCGTCGACGCCCAGGGTCGCACCTTCCTCAAGATTTCCCCGCCGATCCGCCGTACC
AAGGTGATTCCCGAGCCCTGGCGCACCAACATCCTGGTGCGCGGCTGGCGTCGGCTGACCGGACGCAGCAAC
CCGCCCAAGCCCAAGCGTGCCCTGCCGCGGGCCCGCTGGCAGCGGGTCGGCTCGCTGCGCCGGTTCATCCTG
CTGTTGTTGATGCTGGCGCAGACCTCGGTCGCCACCTACTACATGAAAGGCATCCTGCCCTACCAGGGCTGG
GCCTTCGTCGACCTGGAGGAACTGGCCCAGCAGAGCCTGCTGGATACCGTCCAGCAGGTGCTGCCCTATGTC
ATCCAGTTCGGCATCCTGGCGCTCTTCGCGATCCTCTTCTGCTGGGTCTCGGCCGGCTTCTGGACCGCGCTG
ATGGGCTTCTGGGAGCTGCTCACCGGGCGCGACCGCTACCGGATCTCCGGCAGCAGCGCCGGCAGCGAGCCG
ATCGCCGCCGACGCCCGCACGGCGATCGTCATGCCGATCTGCAACGAAGACGTGCCGCGGGTATTCGCCGGC
CTGCGGGCGACCGTCGAGTCGATGGCCGCCACCGGCGAGATGGAACGCTTCGACTTCTTCGTCCTCAGCGAC
ACCAACGACCCGGATATCGCCGTCGCCGAGCAGCAGGCCTGGCTCGAGCTGTGCCGCGAGACCAAGGGCTTC
GGCAAGATCTTCTACCGTCGCCGCCGGCGCCGGGTGAAGCGCAAGAGCGGCAACATCGACGACTTCTGCCGG
CGCTGGGGCGGCGACTACCGCTACATGGTGGTGATGGACGCCGACAGCGTGATGAGCGGCGACTGCCTGGCC
AAGCTGGTGCGCCTGATGGAGGCCAATCCTGAGGCGGGGATCATCCAGACCGCGCCGAAGGCGTCCGGCATG
GACACCCTGTATGCGCGCATGCAGCAGTTCGCCACCCGCGTCTACGGCCCGCTGTTCACCGCCGGcCTGCAC
TTCTGGCAACTCGGCGAGTCGCACTACTGGGGCCACAACGCGATCATCCGCATGCAGCCCTTCATCGACCAC
TGCGCCCCTGGCGCCGTTGCCGGGCAAGGGCTCGTTCGCCGGCGCGATCCTGTCCCACGACTTCGTCGAGGC
TGCGCTGATGCGCCGTGCCGGCTGGGGCGTGTGGATCGCCTACGACCTCGACGGCAGCTACGAAGAACTGCC
GCCGAACCTGCTCGACGAACTCAAGCGCGACCGCCGCTGGTGCCACGGCAACCTGATGAACTTCCGCCTGTT
CCTGGTCAAGGGCATGCACCCGGTGCACCGCGCGGTGTTCCTCACCGGGGTCATGTCCTACCTGTCGGCGCC
GTTGTGGTTCTTCTTCCTGGTGCTTTCCACCGCGCTGCTGGCGGTGCACCAGCTGATGGAGCCGCAGTACTT
CCTGGAACCGCGGCAGTTGTTCCCGATCTGGCCGCAGTGGCATCCGGAGAAGGCCATCGCGTTGTTCTCCAC
CACCCTGACCCTGTTGTTCCTGCCCAAGCTGCTCAGCGTAATGCTGATCTGGGCCAAGGGCGCCAAGGGTTT
TGGCGGGGTGATCCGAGTGACCCTGAGCATGCTCCTGGAGATGTTCTTCTCGGTGCTGCTGGCGCCGGTGCG
CATGCTCTTCCACACCCGCTTCGTGCTGGCCGCCTTCCTCGGCTGGTCGGTGCAGTGGAACTCGCCGCAGCG
CGACGACGACGCCACGCCCTGGAGCGAGGCGATCCGCCGGCACGGCATGCAGACCCTGCTAGGTATCGCCTG
GACCCTGCTGGTGGCCTGGCTCAACCCGCGCTTCCTGTGGTGGCTGTCGCCGATCGTCGGTTCGCTGATCCT
GTCGATCCCGGTATCGGTGATCTCCAGCCGGGTGAAGCTGGGCCTGCGCGCCCGCGATGA
Translation:
MNNPSTTKAPLADYLAHLPLAEEERERLGESASFSELHARLAGAEGAAADAGGDPALASVRARLQLGTPELD
DAEMFGVDAQGRTFLKISPPIRRTKVIPEPWRTNILVRGWRRLTGRSNPPKPKRALPRARWQRVGSLRRFIL
LLLMLAQTSVATYYMKGILPYQGWAFVDLEELAQQSLLDTVQQVLPYVIQFGILALFAILFCWVSAGFWTAL
MGFWELLTGRDRYRISGSSAGSEPIAADARTAIVMPICNEDVPRVFAGLRATVESMAATGEMERFDFFVLSD
TNDPDIAVAEQQAWLELCRETKGFGKIFYRRRRRRVKRKSGNIDDFCRRWGGDYRYMVVMDADSVMSGDCLA
KLVRLMEANPEAGIIQTAPKASGMDTLYARMQQFATRVYGPLFTAGLHFWQLGESHYWGHNAIIRMQPFIDH
CAPGAVAGQGLVRRRDPVPRLRRGCADAPCRLGRVDRLRPRRQLRRTAAEPARRTQARPPLVPRQPDELPPV
PGQGHAPGAPRGVPHRGHVLPVGAVVVLLPGAFHRAAGGAPADGAAVLPGTAAVVPDLAAVASGEGHRVVLH
HPDPVVPAQAAQRNADLGQGRQGFWRGDPSDPEHAPGDVLLGAAGAGAHALPHPLRAGRLPRLVGAVELAAA
RRRRHALERGDPPARHADPARYRLDPAGGLAQPALPVVAVADRRFADPVDPGIGDLQPGEAGPARPR*
AnnotationID:6849GeneID:6848
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 18:06:52
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:membrane glycosyltransferase; synthesis of membrane-derived oligosaccharide
Functional Category Confidence Code:5
COGs:COG2943
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:2.4.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70590.1|AE016701_10 (AE016792) periplasmic glucans biosynthesis protein [Pseudomonas
           putida KT2440]
          Length = 857

 Score =  669 bits (1726), Expect = 0.0
 Identities = 327/444 (73%), Positives = 377/444 (84%), Gaps = 5/444 (1%)

>gb|AAO58587.1| (AE016874) periplasmic glucan biosynthesis protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 859

 Score =  664 bits (1714), Expect = 0.0
 Identities = 324/447 (72%), Positives = 373/447 (83%), Gaps = 8/447 (1%)

>gb|AAG55795.1|AE005315_9 (AE005315) membrane glycosyltransferase; synthesis of
           membrane-derived oligosaccharide (MDO) [Escherichia coli
           O157:H7 EDL933]
          Length = 847

 Score =  511 bits (1317), Expect = e-144
 Identities = 259/437 (59%), Positives = 321/437 (73%), Gaps = 10/437 (2%)








 
Structural Features:
COG2943, MdoH, Membrane glycosyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 736 residues, only  46.3% aligned
Score =  489 bits (1259), Expect = 6e-139
Identities = 229/380 (60%), Positives = 263/380 (69%), Gaps = 40/380 (10%)






   
Genomic Context:






   
Comment:
Inactivated 2/01/06.  PA14_67070 (GeneID 6848) and PA14_67060 (GeneID 6847) 
replaced by PA14_67065 (GeneID 7383).


















    ReferenceID:13410




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30779




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35028




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6848_cdd.html


    		




 
URL:

















    ReferenceID:35820




  
Author/Investigator(s):
        Loubens I, Debarbieux L, Bohin A, Lacroix JM, Bohin JP.





  
Title:
        Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae.




  
PubMed:
        7934824


MedLine:
        




    
Source:
Mol Microbiol. 1993 Oct;10(2):329-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7934824

















    ReferenceID:35823




  
Author/Investigator(s):
        Debarbieux L, Bohin A, Bohin JP.





  
Title:
        Topological analysis of the membrane-bound glucosyltransferase, MdoH, required for osmoregulated periplasmic glucan synthesis in Escherichia coli.




  
PubMed:
        9352918


MedLine:
        




    
Source:
J Bacteriol. 1997 Nov;179(21):6692-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9352918









           
Homologs By Global Alignment 

           

       



     
Gene ID:6848
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8044
                           PA5077_tr
                           translation of PA5077
                           898
                           57.68
                           61.46
                           220
                           2276.0
     

     
     
202678
                           gb|AAG08462.1|AE004920_12
                           (AE004920) periplasmic glucans biosynthesis protein MdoH [Pseudomonas aeruginosa PAO1]
                           898
                           57.68
                           61.46
                           220
                           2276.0
     

     
     
202679
                           gb|AAN70590.1|AE016701_10
                           (AE016792) periplasmic glucans biosynthesis protein [Pseudomonas putida KT2440]
                           910
                           44.39
                           53.18
                           248
                           1738.0
     

     
     
202680
                           gb|AAO58587.1|
                           (AE016874) periplasmic glucan biosynthesis protein [Pseudomonas syringae pv. tomato str. DC3000]
                           913
                           44.35
                           52.57
                           252
                           1718.0
     

     
     
202681
                           emb|CAC13111.1|
                           (AJ294718) OpgH protein [Erwinia chrysanthemi]
                           897
                           38.68
                           48.82
                           229
                           1432.0
     

     






      

          
GC ORFID: 49850How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5251924Stop: 5249777

          
Length: 2148


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5077 translation of PA5077Homolog Bit Score 884.0

          
Other Homologs:  t_PA5077 translation of PA5077 (BLASTP 884), psyr_15may02_Scaffold2_revised_gene3274 related to: gi|140793|sp|P20401|HRPM_PSESY HRPM PROTEIN pir||B31383 hrpM protein - Pseudomonas syringae pv. syringae (BLASTP 663)

          
GC ORF Sequence

           
ATGAATAACCCATCCACTACGAAAGCACCGCTGGCCGACTACCTCGCTCATCTTCCCCTGGCGGAAGAGGAG 
CGGGAGCGGCTTGGCGAGTCCGCTTCCTTCTCCGAGCTGCACGCTCGCCTGGCGGGAGCGGAAGGCGCCGCT 
GCCGATGCCGGGGGCGATCCCGCCCTGGCCTCGGTACGCGCCCGCCTGCAGCTGGGCACCCCTGAGCTGGAC 
GACGCCGAGATGTTCGGCGTCGACGCCCAGGGTCGCACCTTCCTCAAGATTTCCCCGCCGATCCGCCGTACC 
AAGGTGATTCCCGAGCCCTGGCGCACCAACATCCTGGTGCGCGGCTGGCGTCGGCTGACCGGACGCAGCAAC 
CCGCCCAAGCCCAAGCGTGCCCTGCCGCGGGCCCGCTGGCAGCGGGTCGGCTCGCTGCGCCGGTTCATCCTG 
CTGTTGTTGATGCTGGCGCAGACCTCGGTCGCCACCTACTACATGAAAGGCATCCTGCCCTACCAGGGCTGG 
GCCTTCGTCGACCTGGAGGAACTGGCCCAGCAGAGCCTGCTGGATACCGTCCAGCAGGTGCTGCCCTATGTC 
ATCCAGTTCGGCATCCTGGCGCTCTTCGCGATCCTCTTCTGCTGGGTCTCGGCCGGCTTCTGGACCGCGCTG 
ATGGGCTTCTGGGAGCTGCTCACCGGGCGCGACCGCTACCGGATCTCCGGCAGCAGCGCCGGCAGCGAGCCG 
ATCGCCGCCGACGCCCGCACGGCGATCGTCATGCCGATCTGCAACGAAGACGTGCCGCGGGTATTCGCCGGC 
CTGCGGGCGACCGTCGAGTCGATGGCCGCCACCGGCGAGATGGAACGCTTCGACTTCTTCGTCCTCAGCGAC 
ACCAACGACCCGGATATCGCCGTCGCCGAGCAGCAGGCCTGGCTCGAGCTGTGCCGCGAGACCAAGGGCTTC 
GGCAAGATCTTCTACCGTCGCCGCCGGCGCCGGGTGAAGCGCAAGAGCGGCAACATCGACGACTTCTGCCGG 
CGCTGGGGCGGCGACTACCGCTACATGGTGGTGATGGACGCCGACAGCGTGATGAGCGGCGACTGCCTGGCC 
AAGCTGGTGCGCCTGATGGAGGCCAATCCTGAGGCGGGGATCATCCAGACCGCGCCGAAGGCGTCCGGCATG 
GACACCCTGTATGCGCGCATGCAGCAGTTCGCCACCCGCGTCTACGGCCCGCTGTTCACCGCCGGcCTGCAC 
TTCTGGCAACTCGGCGAGTCGCACTACTGGGGCCACAACGCGATCATCCGCATGCAGCCCTTCATCGACCAC 
TGCGCCCCTGGCGCCGTTGCCGGGCAAGGGCTCGTTCGCCGGCGCGATCCTGTCCCACGACTTCGTCGAGGC 
TGCGCTGATGCGCCGTGCCGGCTGGGGCGTGTGGATCGCCTACGACCTCGACGGCAGCTACGAAGAACTGCC 
GCCGAACCTGCTCGACGAACTCAAGCGCGACCGCCGCTGGTGCCACGGCAACCTGATGAACTTCCGCCTGTT 
CCTGGTCAAGGGCATGCACCCGGTGCACCGCGCGGTGTTCCTCACCGGGGTCATGTCCTACCTGTCGGCGCC 
GTTGTGGTTCTTCTTCCTGGTGCTTTCCACCGCGCTGCTGGCGGTGCACCAGCTGATGGAGCCGCAGTACTT 
CCTGGAACCGCGGCAGTTGTTCCCGATCTGGCCGCAGTGGCATCCGGAGAAGGCCATCGCGTTGTTCTCCAC 
CACCCTGACCCTGTTGTTCCTGCCCAAGCTGCTCAGCGTAATGCTGATCTGGGCCAAGGGCGCCAAGGGTTT 
TGGCGGGGTGATCCGAGTGACCCTGAGCATGCTCCTGGAGATGTTCTTCTCGGTGCTGCTGGCGCCGGTGCG 
CATGCTCTTCCACACCCGCTTCGTGCTGGCCGCCTTCCTCGGCTGGTCGGTGCAGTGGAACTCGCCGCAGCG 
CGACGACGACGCCACGCCCTGGAGCGAGGCGATCCGCCGGCACGGCATGCAGACCCTGCTAGGTATCGCCTG 
GACCCTGCTGGTGGCCTGGCTCAACCCGCGCTTCCTGTGGTGGCTGTCGCCGATCGTCGGTTCGCTGATCCT 
GTCGATCCCGGTATCGGTGATCTCCAGCCGGGTGAAGCTGGGCCTGCGCGCCCGCGATGA