ViewGene
Locus Name:
GeneID:
Location:

GeneID:6847
TrivialName: PA14_67060
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67060
PAO1 Ortholog Locus:
Sequence Length:1425
Protein Length:474
Start: 5987077
Stop: 5985654
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/01/06. PA14_67070 (GeneID 6848) and PA14_67060 (GeneID 6847) replaced by PA14_67065 (GeneID 7383). Homology: gb|AAG08462.1|AE004920_12 (AE004920) periplasmic glucans biosynthesis protein MdoH [Pseudomonas aeruginosa PAO1]
Identities = 426/427 (99%), Positives = 427/427 (100%)
Sequence:
ATGCGCGCATGCAGCAGTTCGCCACCCGCGTCTACGGCCCGCTGTTCACCGCCGGcCTGCACTTCTGGCAAC
TCGGCGAGTCGCACTACTGGGGCCACAACGCGATCATCCGCATGCAGCCCTTCATCGACCACTGCGCCCCTG
GCGCCGTTGCCGGGCAAGGGCTCGTTCGCCGGCGCGATCCTGTCCCACGACTTCGTCGAGGCTGCGCTGATG
CGCCGTGCCGGCTGGGGCGTGTGGATCGCCTACGACCTCGACGGCAGCTACGAAGAACTGCCGCCGAACCTG
CTCGACGAACTCAAGCGCGACCGCCGCTGGTGCCACGGCAACCTGATGAACTTCCGCCTGTTCCTGGTCAAG
GGCATGCACCCGGTGCACCGCGCGGTGTTCCTCACCGGGGTCATGTCCTACCTGTCGGCGCCGTTGTGGTTC
TTCTTCCTGGTGCTTTCCACCGCGCTGCTGGCGGTGCACCAGCTGATGGAGCCGCAGTACTTCCTGGAACCG
CGGCAGTTGTTCCCGATCTGGCCGCAGTGGCATCCGGAGAAGGCCATCGCGTTGTTCTCCACCACCCTGACC
CTGTTGTTCCTGCCCAAGCTGCTCAGCGTAATGCTGATCTGGGCCAAGGGCGCCAAGGGTTTTGGCGGGGTG
ATCCGAGTGACCCTGAGCATGCTCCTGGAGATGTTCTTCTCGGTGCTGCTGGCGCCGGTGCGCATGCTCTTC
CACACCCGCTTCGTGCTGGCCGCCTTCCTCGGCTGGTCGGTGCAGTGGAACTCGCCGCAGCGCGACGACGAC
GCCACGCCCTGGAGCGAGGCGATCCGCCGGCACGGCATGCAGACCCTGCTAGGTATCGCCTGGACCCTGCTG
GTGGCCTGGCTCAACCCGCGCTTCCTGTGGTGGCTGTCGCCGATCGTCGGTTCGCTGATCCTGTCGATCCCG
GTATCGGTGATCTCCAGCCGGGTGAAGCTGGGCCTGCGCGCCCGCGATGAAAAGCTGTTCCTGATCCCGGAG
GAGTACGACACGCCGCGCGAGCTGCGCGCCACCGACGAGTACACCTACGAGAACCGCTGGCATGCGCTCAAG
GATGGCTTCCTCAAGGCCGCCGTCGATCCGTTGCTCAACGCCCTGGCCTGCGCCATGGGCACGGCTCGCCAC
AACCGTGCGCAAGCCATCGAGACGGTGCGCGGCGAGCGTATCGGCAAGGCCATCGAGAAGGGCCCGGAACAG
CTCGACGGCGCCACGCGCCTGGCCCTGTTGAGTGACCCGGTAGCACTTTCGCGCCTGCATACGCGGGTCTGG
GAAGAGGACCGCGACGACTGGCTCGGCCGCTGGCGCAAGGCCGAGGCGGACGACCCCCACGCCGCCAGCGTA
CCGCTGGCCCAGGTAGTGCCCGGCGACGCCGGCCTGCTGCCCGCCGCCCAGTCCTGA
Translation:
MRACSSSPPASTARCSPPACTSGNSASRTTGATTRSSACSPSSTTAPLAPLPGKGSFAGAILSHDFVEAALM
RRAGWGVWIAYDLDGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWF
FFLVLSTALLAVHQLMEPQYFLEPRQLFPIWPQWHPEKAIALFSTTLTLLFLPKLLSVMLIWAKGAKGFGGV
IRVTLSMLLEMFFSVLLAPVRMLFHTRFVLAAFLGWSVQWNSPQRDDDATPWSEAIRRHGMQTLLGIAWTLL
VAWLNPRFLWWLSPIVGSLILSIPVSVISSRVKLGLRARDEKLFLIPEEYDTPRELRATDEYTYENRWHALK
DGFLKAAVDPLLNALACAMGTARHNRAQAIETVRGERIGKAIEKGPEQLDGATRLALLSDPVALSRLHTRVW
EEDRDDWLGRWRKAEADDPHAASVPLAQVVPGDAGLLPAAQS*
AnnotationID:6848GeneID:6847
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 18:06:30
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:membrane glycosyltransferase; synthesis of membrane-derived oligosaccharide
Functional Category Confidence Code:5
COGs:COG2943
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:2.4.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70590.1|AE016701_10 (AE016792) periplasmic glucans biosynthesis protein [Pseudomonas
           putida KT2440]
          Length = 857

 Score =  686 bits (1771), Expect = 0.0
 Identities = 329/424 (77%), Positives = 366/424 (86%)

>gb|AAO58587.1| (AE016874) periplasmic glucan biosynthesis protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 859

 Score =  684 bits (1765), Expect = 0.0
 Identities = 325/415 (78%), Positives = 361/415 (86%)

>gb|AAC74133.1| (AE000206) membrane glycosyltransferase; synthesis of
           membrane-derived oligosaccharide (MDO) [Escherichia coli
           K12]
          Length = 847

 Score =  562 bits (1448), Expect = e-159
 Identities = 273/385 (70%), Positives = 313/385 (81%)








 
Structural Features:
COG2943, MdoH, Membrane glycosyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 736 residues, only  55.7% aligned
Score =  605 bits (1562), Expect = 3e-174
Identities = 269/410 (65%), Positives = 311/410 (75%), Gaps = 4/410 (0%)






   
Genomic Context:






   
Comment:
Inactivated 2/01/06.  PA14_67070 (GeneID 6848) and PA14_67060 (GeneID 6847) 
replaced by PA14_67065 (GeneID 7383).


















    ReferenceID:13408




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30777




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35029




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6847_cdd.html


    		




 
URL:

















    ReferenceID:35821




  
Author/Investigator(s):
        Loubens I, Debarbieux L, Bohin A, Lacroix JM, Bohin JP.





  
Title:
        Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae.




  
PubMed:
        7934824


MedLine:
        




    
Source:
Mol Microbiol. 1993 Oct;10(2):329-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7934824

















    ReferenceID:35822




  
Author/Investigator(s):
        Debarbieux L, Bohin A, Bohin JP.





  
Title:
        Topological analysis of the membrane-bound glucosyltransferase, MdoH, required for osmoregulated periplasmic glucan synthesis in Escherichia coli.




  
PubMed:
        9352918


MedLine:
        




    
Source:
J Bacteriol. 1997 Nov;179(21):6692-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9352918









           
Homologs By Global Alignment 

           

       



     
Gene ID:6847
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8043
                           PA5077_tr
                           translation of PA5077
                           861
                           50.52
                           51.33
                           387
                           2249.0
     

     
     
202658
                           gb|AAG08462.1|AE004920_12
                           (AE004920) periplasmic glucans biosynthesis protein MdoH [Pseudomonas aeruginosa PAO1]
                           861
                           50.52
                           51.33
                           387
                           2249.0
     

     
     
202659
                           gb|AAN70590.1|AE016701_10
                           (AE016792) periplasmic glucans biosynthesis protein [Pseudomonas putida KT2440]
                           860
                           39.30
                           44.18
                           389
                           1768.0
     

     
     
202660
                           gb|AAO58587.1|
                           (AE016874) periplasmic glucan biosynthesis protein [Pseudomonas syringae pv. tomato str. DC3000]
                           863
                           38.81
                           43.80
                           393
                           1760.0
     

     
     
202664
                           emb|CAD08275.1|
                           (AL627269) periplasmic glucans biosynthesis protein MdoH [Salmonella enterica subsp. enterica serovar Typhi]
                           874
                           34.09
                           39.47
                           427
                           1461.0
     

     






      

          
GC ORFID: 49849How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5250762Stop: 5249338

          
Length: 1425


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5077 translation of PA5077Homolog Bit Score 871.0

          
Other Homologs:  t_PA5077 translation of PA5077 (BLASTP 871), psyr_15may02_Scaffold2_revised_gene3274 related to: gi|140793|sp|P20401|HRPM_PSESY HRPM PROTEIN pir||B31383 hrpM protein - Pseudomonas syringae pv. syringae (BLASTP 681)

          
GC ORF Sequence

           
ATGCGCGCATGCAGCAGTTCGCCACCCGCGTCTACGGCCCGCTGTTCACCGCCGGcCTGCACTTCTGGCAAC 
TCGGCGAGTCGCACTACTGGGGCCACAACGCGATCATCCGCATGCAGCCCTTCATCGACCACTGCGCCCCTG 
GCGCCGTTGCCGGGCAAGGGCTCGTTCGCCGGCGCGATCCTGTCCCACGACTTCGTCGAGGCTGCGCTGATG 
CGCCGTGCCGGCTGGGGCGTGTGGATCGCCTACGACCTCGACGGCAGCTACGAAGAACTGCCGCCGAACCTG 
CTCGACGAACTCAAGCGCGACCGCCGCTGGTGCCACGGCAACCTGATGAACTTCCGCCTGTTCCTGGTCAAG 
GGCATGCACCCGGTGCACCGCGCGGTGTTCCTCACCGGGGTCATGTCCTACCTGTCGGCGCCGTTGTGGTTC 
TTCTTCCTGGTGCTTTCCACCGCGCTGCTGGCGGTGCACCAGCTGATGGAGCCGCAGTACTTCCTGGAACCG 
CGGCAGTTGTTCCCGATCTGGCCGCAGTGGCATCCGGAGAAGGCCATCGCGTTGTTCTCCACCACCCTGACC 
CTGTTGTTCCTGCCCAAGCTGCTCAGCGTAATGCTGATCTGGGCCAAGGGCGCCAAGGGTTTTGGCGGGGTG 
ATCCGAGTGACCCTGAGCATGCTCCTGGAGATGTTCTTCTCGGTGCTGCTGGCGCCGGTGCGCATGCTCTTC 
CACACCCGCTTCGTGCTGGCCGCCTTCCTCGGCTGGTCGGTGCAGTGGAACTCGCCGCAGCGCGACGACGAC 
GCCACGCCCTGGAGCGAGGCGATCCGCCGGCACGGCATGCAGACCCTGCTAGGTATCGCCTGGACCCTGCTG 
GTGGCCTGGCTCAACCCGCGCTTCCTGTGGTGGCTGTCGCCGATCGTCGGTTCGCTGATCCTGTCGATCCCG 
GTATCGGTGATCTCCAGCCGGGTGAAGCTGGGCCTGCGCGCCCGCGATGAAAAGCTGTTCCTGATCCCGGAG 
GAGTACGACACGCCGCGCGAGCTGCGCGCCACCGACGAGTACACCTACGAGAACCGCTGGCATGCGCTCAAG 
GATGGCTTCCTCAAGGCCGCCGTCGATCCGTTGCTCAACGCCCTGGCCTGCGCCATGGGCACGGCTCGCCAC 
AACCGTGCGCAAGCCATCGAGACGGTGCGCGGCGAGCGTATCGGCAAGGCCATCGAGAAGGGCCCGGAACAG 
CTCGACGGCGCCACGCGCCTGGCCCTGTTGAGTGACCCGGTAGCACTTTCGCGCCTGCATACGCGGGTCTGG 
GAAGAGGACCGCGACGACTGGCTCGGCCGCTGGCGCAAGGCCGAGGCGGACGACCCCCACGCCGCCAGCGTA 
CCGCTGGCCCAGGTAGTGCCCGGCGACGCCGGCCTGCTGCCCGCCGCCCAGTCCTGA