ViewGene
Locus Name:
GeneID:
Location:

GeneID:6843
TrivialName: PA14_66870
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66870
PAO1 Ortholog Locus:
Sequence Length:504
Protein Length:167
Start: 5973071
Stop: 5972569
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
GeneID6843 (PA14_66870) replaced by 7423 (PA14_66875). Homology: gb|AAG08445.1|AE004919_6 (AE004919) polyhydroxyalkanoate synthesis protein PhaF [Pseudomonas aeruginosa PAO1]
Identities = 138/147 (93%), Positives = 142/147 (96%)
Sequence:
ATGGCTGGCAAGAAGAAGAGCGAAAAAGAGTCCAGTTGGATCGGCGAGATCGAGAAATACTCGCGGCAGATC
TGGCTGGCTGGTCTGGGGGCCTACTCGAAGGTCAGCAAGGACGGCAGCAAGCTGTTCGAGACCTTGGTGAAA
GACGGCGAGAAGGCTGAAAAAGAAGCGAAGTCCGATGTGGACGCGCAGGTCGGTGCGGCGAAGGCTTCCGCC
CGCTCTGCGAAGAGCAAGGTTGACGAGGTTCGGGATCGTGCGCTTGGCAAGTGGAGCGAGCTCGAGGAAGCT
TTCGACAAGCGCCTGAACAGCGCCATCTCGCGTCTCGGCGTGCCGAGCCGCAACGAGGTGAAGGAGCTGCAC
GGCAAGGTCGATACGCTGACCAGGCAGATCGAGAAACTCACCGGCGTCaGcgTCAAgcCCGGCGGCGAAGgc
aGCGGCCAAGCCTGCGGCGAAACCGGCTGCCAAGCCCGCGGCGAAAACCGCAGCGGCCAAGCCGGCAGCTAA
Translation:
MAGKKKSEKESSWIGEIEKYSRQIWLAGLGAYSKVSKDGSKLFETLVKDGEKAEKEAKSDVDAQVGAAKASA
RSAKSKVDEVRDRALGKWSELEEAFDKRLNSAISRLGVPSRNEVKELHGKVDTLTRQIEKLTGVSVKPGGEG
SGQACGETGCQARGENRSGQAGS*
AnnotationID:6844GeneID:6843
AnnotatorUID: danlee
Modification Date/Time: 2006-03-13 12:12:25
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam05597
Secondary Functional Category(ies):
EC Number:
Status:INACTIVE
Pathway:






 
Homology:
>dbj|BAB91367.1| (AB073926) protein associated to the polyhydroxyalkanoate inclusion
           [Pseudomonas sp. 61-3]
          Length = 253

 Score =  213 bits (541), Expect = 5e-55
 Identities = 109/141 (77%), Positives = 122/141 (86%), Gaps = 1/141 (0%)

>gb|AAO58573.1| (AE016874) polyhydroxyalkanoate granule-associated protein PhaF
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 257

 Score =  206 bits (523), Expect = 6e-53
 Identities = 103/141 (73%), Positives = 120/141 (85%), Gaps = 1/141 (0%)

>gb|AAN70573.1|AE016700_1 (AE016792) polyhydroxyalkanoate granule-associated protein GA2
           [Pseudomonas putida KT2440]
          Length = 261

 Score =  193 bits (491), Expect = 3e-49
 Identities = 100/141 (70%), Positives = 116/141 (82%), Gaps = 4/141 (2%)









 
Structural Features:
pfam05597, Phasin, Poly(hydroxyalcanoate) granule associated protein (phasin). 
CD-Length = 132 residues, 100.0% aligned
Score =  145 bits (367), Expect = 3e-36
Identities = 80/135 (59%), Positives = 106/135 (78%), Gaps = 4/135 (2%)






   
Genomic Context:






   
Comment:
GeneID6843 (PA14_66870) replaced by 7423 (PA14_66875).


















    ReferenceID:13400




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30769




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35002




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6843_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6843
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8041
                           PA5060_tr
                           translation of PA5060
                           309
                           44.66
                           47.89
                           142
                           689.0
     

     
     
202648
                           gb|AAG08445.1|AE004919_6
                           (AE004919) polyhydroxyalkanoate synthesis protein PhaF [Pseudomonas aeruginosa PAO1]
                           309
                           44.66
                           47.89
                           142
                           689.0
     

     
     
202649
                           dbj|BAB91367.1|
                           (AB073926) protein associated to the polyhydroxyalkanoate inclusion [Pseudomonas sp. 61-3]
                           253
                           43.87
                           51.38
                           86
                           531.0
     

     
     
202651
                           gb|AAO58573.1|
                           (AE016874) polyhydroxyalkanoate granule-associated protein PhaF [Pseudomonas syringae pv. tomato str. DC3000]
                           257
                           41.24
                           50.58
                           90
                           512.0
     

     
     
202652
                           emb|CAA09109.1|
                           (AJ010393) phaF protein [Pseudomonas oleovorans]
                           260
                           40.38
                           47.69
                           98
                           490.0
     

     






      

          
GC ORFID: 49845How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5236755Stop: 5236252

          
Length: 504


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5060 translation of PA5060Homolog Bit Score 270.0

          
Other Homologs:  t_PA5060 translation of PA5060 (BLASTP 270), psyr_15may02_Scaffold2_revised_gene3281 related to: gi|17402515|dbj|BAB78723.1| (AB049413) phaF [Pseudomonas chlororaphis] (BLASTP 201), t_PA5061 translation of PA5061 (BLASTP 110)

          
GC ORF Sequence

           
ATGGCTGGCAAGAAGAAGAGCGAAAAAGAGTCCAGTTGGATCGGCGAGATCGAGAAATACTCGCGGCAGATC 
TGGCTGGCTGGTCTGGGGGCCTACTCGAAGGTCAGCAAGGACGGCAGCAAGCTGTTCGAGACCTTGGTGAAA 
GACGGCGAGAAGGCTGAAAAAGAAGCGAAGTCCGATGTGGACGCGCAGGTCGGTGCGGCGAAGGCTTCCGCC 
CGCTCTGCGAAGAGCAAGGTTGACGAGGTTCGGGATCGTGCGCTTGGCAAGTGGAGCGAGCTCGAGGAAGCT 
TTCGACAAGCGCCTGAACAGCGCCATCTCGCGTCTCGGCGTGCCGAGCCGCAACGAGGTGAAGGAGCTGCAC 
GGCAAGGTCGATACGCTGACCAGGCAGATCGAGAAACTCACCGGCGTCaGcgTCAAgcCCGGCGGCGAAGgc 
aGCGGCCAAGCCTGCGGCGAAACCGGCTGCCAAGCCCGCGGCGAAAACCGCAGCGGCCAAGCCGGCAGCTAA