ViewGene
Locus Name:
GeneID:
Location:

GeneID:6820
TrivialName: PA14_65790
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65790
PAO1 Ortholog Locus:
Sequence Length:2043
Protein Length:680
Start: 5860649
Stop: 5858608
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
GeneID6819 (PA14_65800) and GeneID6820 (PA14_65790) replaced by GeneID7422 (PA14_65795). Homology: gb|AAG08362.1|AE004910_9 (AE004910) probable acetolactate synthase large subunit [Pseudomonas aeruginosa PAO1]
Identities = 259/260 (99%), Positives = 259/260 (99%)
Sequence:
ATGGGAGCAAGAGCGCTGCGGCGCGAGCGGCGACTCCTATGGTCGCCGAACTGGACGAGGATATTGCCCATG
CAACCACAGAAGACCCTCACCGCCGGCCAGGCCCTGGTCCGCTTGCTGGCCAACTACGGCGTGGATACCGTT
TTCGGTATCCCCGGCGTGCACACCCTCGAGCTGTACCGCGGACTTCCGGGCAGCGGCATCCGCCACGTGCTG
ACCCGCCACGAGCAGGGCGCCGGCTTCATGGCCGACGGTTATGCGCGGGTCAGCGGCAAGCCGGGAGTGTGC
TTCGTCATCACCGGGCCGGGCGTGACCAACGTCGCCACCGCCATCGGCCAGGCCTACGCCGACTCGGTACCG
CTGCTGGTGATTTCCAGCGTCAACCACAGCGCCAGCCTGGGCAAGGGCTGGGGCTGCCTGCACGAGACCCAG
GACCAGCGTGCCATGACCGCGCCGATCACTGCCTTCTCCGCCTTGGCCTTGAGCCCGGAACAGCTGCCCGAA
CTGATCGCCCGGGCCTACGCGGTGTTCGACAGCGAGCGTCCGCGCCCGGTGCATATCTCGATCCCGCTCGAC
GTGCTCGCCGCGCCGGTGGCCCATGACTGGAGCGCTGCCGTGGCGCGCCGCCCTGGTCGTGGTGTGCCGTGC
AGCGAGGCATTGCGGGCGGCGGCGGAGCGTCTGGCTGCGGCCCGGCGACCAATGCTCATCGCCGGCGGCGGC
GCGCTGGCGgccgGCGAGGCGctTgccGCGCTGAGCGAGCGCCTGGCCGCGCCGCTGTttcACCAGCGTCGC
cggCAAGGGTCTGCTGCCGCcgGACGCGCCGCTCAACGCCGGGGCCAGCCTGTGCGTAGCGCCGGGCTGGGA
GATGATCGCCGAGGCCGACCTGGTGCTGGCGGTGGGCACCGAGATGGCCGACACCGACTTCTGGCGCGAACG
CCTGCCGCTGTCCGGCGAACTGATCCGCGTCGATATCGACCCGCGCAAGTTCAACGACTTCTATCCCTCTGC
CGTAGCGCTCAGGGGCGATGCCCGGCAGACCCTGGAAGCGCTGCTGGCGCGCCTGCCGCAGGAGGCCCGCGA
CGCCGCGCCGGCCGCCGCGAGGGTGGCGCGCCTGCGCGCGGAGATCCGCGCGGCACACGCGCCGCTGCAGGC
GCTGCACCAGGCGATCCTCGACCGGATCGCCGCTGCGCTGCCGGCCGATGCGTTCGTCAGCACCGACATGAC
CCAACTGGCCTATACCGGCAACTACGCGTTCGCCAGCCGAGCACCGCGCAGCTGGCTGCATCCCACCGGCTA
CGGCACCCTCGGCTACGGCCTGCCGGCCGGCATCGGCGCCAAGCTCGGCGCCCCGCAGCGCCCGGGCCTGGT
GCTGGTCGGCGATGGCGGCTTCCTCTACACCGCGCAGGAACTGGCCACCGCCAGCGAGGAACTGGACAGTCC
GCTGGTGGTGCTACTGTGGAACAACGACGCGCTCGGCCAGATCCGCGACGACATGCTCGGCCTGGACATCGA
GCCGGTCGGCGTGCTGCCGCGCAACCCCGACTTCGCCCTGCTGGGCCGCGCCTACGGTTGCGCGGTGCGCCA
GCCGCAGGACCTGGACGAACTGGAGCGCGACCTGCGCGCCGGCTTCGGGCAGCCCGGCGTGACCCTGATCGA
ACTGCGGCACGCCTGCGCGCGCTGACCGCCGGACAAGGAGAGCATCCATGCGCTATTCCGACTTCACCCAAC
GTATCGCCGGCGACGGCGCCGCTGCCTGGGACATCCACTATCGCGCGCTGGCGCGGGTCGAGCAGGGCGAGG
AAATCCTCCTGCTGTCGGTCGGCGACCCCGACTTCGATACCCCGGCGCCCATCGTCCAGTCGGCCATCGACA
GCCTGCTGGCCGGCAACACCCACTACGCCGACGTGCGCGGCAAGCGCGCCCTGCGCCAGCGCATCGCCGAAC
GCCACCGACAGCGCAGCGGCCAGGCGGTGGACGCGGAGCAGGTGGTGGTGCTGGCCGGCGCCCAGTGCGCGC
TGTACGCGGTGGTGCAGTGCCTGCTGA
Translation:
MGARALRRERRLLWSPNWTRILPMQPQKTLTAGQALVRLLANYGVDTVFGIPGVHTLELYRGLPGSGIRHVL
TRHEQGAGFMADGYARVSGKPGVCFVITGPGVTNVATAIGQAYADSVPLLVISSVNHSASLGKGWGCLHETQ
DQRAMTAPITAFSALALSPEQLPELIARAYAVFDSERPRPVHISIPLDVLAAPVAHDWSAAVARRPGRGVPC
SEALRAAAERLAAARRPMLIAGGGALAAGEALAALSERLAAPLFHQRRRQGSAAAGRAAQRRGQPVRSAGLG
DDRRGRPGAGGGHRDGRHRLLARTPAAVRRTDPRRYRPAQVQRLLSLCRSAQGRCPADPGSAAGAPAAGGPR
RRAGRREGGAPARGDPRGTRAAAGAAPGDPRPDRRCAAGRCVRQHRHDPTGLYRQLRVRQPSTAQLAASHRL
RHPRLRPAGRHRRQARRPAAPGPGAGRRWRLPLHRAGTGHRQRGTGQSAGGATVEQRRARPDPRRHARPGHR
AGRRAAAQPRLRPAGPRLRLRGAPAAGPGRTGARPARRLRAARRDPDRTAARLRALTAGQGEHPCAIPTSPN
VSPATAPLPGTSTIARWRGSSRARKSSCCRSATPTSIPRRPSSSRPSTACWPATPTTPTCAASAPCASASPN
ATDSAAARRWTRSRWWCWPAPSARCTRWCSAC*
AnnotationID:6821GeneID:6820
AnnotatorUID: danlee
Modification Date/Time: 2006-03-13 11:11:55
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02776
Secondary Functional Category(ies):coenzyme metabolism
EC Number:
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAN67017.1|AE016330_6 (AE016779) acetolactate synthase, large subunit, putative
           [Pseudomonas putida KT2440]
          Length = 545

 Score =  278 bits (710), Expect = 1e-73
 Identities = 144/232 (62%), Positives = 171/232 (73%), Gaps = 7/232 (3%)

>dbj|BAB51695.1| (AP003006) acetohydroxy acid synthase [Mesorhizobium loti]
          Length = 526

 Score =  251 bits (641), Expect = 1e-65
 Identities = 132/237 (55%), Positives = 161/237 (67%), Gaps = 2/237 (0%)

>emb|CAC45139.1| (AL591784) PUTATIVE ACETOLACTATE SYNTHASE ISOZYME I LARGE SUBUNIT
           PROTEIN [Sinorhizobium meliloti]
          Length = 537

 Score =  230 bits (587), Expect = 2e-59
 Identities = 129/241 (53%), Positives = 153/241 (63%), Gaps = 4/241 (1%)








 
Structural Features:
pfam02776, TPP_enzyme_N, Thiamine pyrophosphate enzyme, N-terminal TPP binding domain.
CD-Length = 171 residues,  97.1% aligned
Score =  161 bits (410), Expect = 2e-40
Identities = 74/169 (43%), Positives = 93/169 (55%), Gaps = 4/169 (2%)






   
Genomic Context:






   
Comment:
GeneID6819 (PA14_65800) and GeneID6820 (PA14_65790) replaced by GeneID7422 (PA14_65795).


















    ReferenceID:13358




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30723




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35115




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6820_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6820
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8036
                           PA4977_tr
                           translation of PA4977
                           760
                           43.55
                           46.44
                           281
                           1334.0
     

     
     
202621
                           gb|AAG08362.1|AE004910_9
                           (AE004910) probable acetolactate synthase large subunit [Pseudomonas aeruginosa PAO1]
                           760
                           43.55
                           46.44
                           281
                           1334.0
     

     
     
202622
                           gb|AAN67017.1|AE016330_6
                           (AE016779) acetolactate synthase, large subunit, putative [Pseudomonas putida KT2440]
                           759
                           28.98
                           36.10
                           293
                           685.0
     

     
     
8037
                           PA1417_tr
                           translation of PA1417
                           768
                           27.99
                           33.98
                           323
                           691.0
     

     
     
202623
                           gb|AAG04806.1|AE004571_5
                           (AE004571) probable decarboxylase [Pseudomonas aeruginosa PAO1]
                           768
                           27.99
                           33.98
                           323
                           691.0
     

     






      

          
GC ORFID: 49822How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5124332Stop: 5122290

          
Length: 2043


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4977 translation of PA4977Homolog Bit Score 514.0

          
Other Homologs:  t_PA4977 translation of PA4977 (BLASTP 514), t_PA1417 translation of PA1417 (BLASTP 267)

          
GC ORF Sequence

           
ATGGGAGCAAGAGCGCTGCGGCGCGAGCGGCGACTCCTATGGTCGCCGAACTGGACGAGGATATTGCCCATG 
CAACCACAGAAGACCCTCACCGCCGGCCAGGCCCTGGTCCGCTTGCTGGCCAACTACGGCGTGGATACCGTT 
TTCGGTATCCCCGGCGTGCACACCCTCGAGCTGTACCGCGGACTTCCGGGCAGCGGCATCCGCCACGTGCTG 
ACCCGCCACGAGCAGGGCGCCGGCTTCATGGCCGACGGTTATGCGCGGGTCAGCGGCAAGCCGGGAGTGTGC 
TTCGTCATCACCGGGCCGGGCGTGACCAACGTCGCCACCGCCATCGGCCAGGCCTACGCCGACTCGGTACCG 
CTGCTGGTGATTTCCAGCGTCAACCACAGCGCCAGCCTGGGCAAGGGCTGGGGCTGCCTGCACGAGACCCAG 
GACCAGCGTGCCATGACCGCGCCGATCACTGCCTTCTCCGCCTTGGCCTTGAGCCCGGAACAGCTGCCCGAA 
CTGATCGCCCGGGCCTACGCGGTGTTCGACAGCGAGCGTCCGCGCCCGGTGCATATCTCGATCCCGCTCGAC 
GTGCTCGCCGCGCCGGTGGCCCATGACTGGAGCGCTGCCGTGGCGCGCCGCCCTGGTCGTGGTGTGCCGTGC 
AGCGAGGCATTGCGGGCGGCGGCGGAGCGTCTGGCTGCGGCCCGGCGACCAATGCTCATCGCCGGCGGCGGC 
GCGCTGGCGgccgGCGAGGCGctTgccGCGCTGAGCGAGCGCCTGGCCGCGCCGCTGTttcACCAGCGTCGC 
cggCAAGGGTCTGCTGCCGCcgGACGCGCCGCTCAACGCCGGGGCCAGCCTGTGCGTAGCGCCGGGCTGGGA 
GATGATCGCCGAGGCCGACCTGGTGCTGGCGGTGGGCACCGAGATGGCCGACACCGACTTCTGGCGCGAACG 
CCTGCCGCTGTCCGGCGAACTGATCCGCGTCGATATCGACCCGCGCAAGTTCAACGACTTCTATCCCTCTGC 
CGTAGCGCTCAGGGGCGATGCCCGGCAGACCCTGGAAGCGCTGCTGGCGCGCCTGCCGCAGGAGGCCCGCGA 
CGCCGCGCCGGCCGCCGCGAGGGTGGCGCGCCTGCGCGCGGAGATCCGCGCGGCACACGCGCCGCTGCAGGC 
GCTGCACCAGGCGATCCTCGACCGGATCGCCGCTGCGCTGCCGGCCGATGCGTTCGTCAGCACCGACATGAC 
CCAACTGGCCTATACCGGCAACTACGCGTTCGCCAGCCGAGCACCGCGCAGCTGGCTGCATCCCACCGGCTA 
CGGCACCCTCGGCTACGGCCTGCCGGCCGGCATCGGCGCCAAGCTCGGCGCCCCGCAGCGCCCGGGCCTGGT 
GCTGGTCGGCGATGGCGGCTTCCTCTACACCGCGCAGGAACTGGCCACCGCCAGCGAGGAACTGGACAGTCC 
GCTGGTGGTGCTACTGTGGAACAACGACGCGCTCGGCCAGATCCGCGACGACATGCTCGGCCTGGACATCGA 
GCCGGTCGGCGTGCTGCCGCGCAACCCCGACTTCGCCCTGCTGGGCCGCGCCTACGGTTGCGCGGTGCGCCA 
GCCGCAGGACCTGGACGAACTGGAGCGCGACCTGCGCGCCGGCTTCGGGCAGCCCGGCGTGACCCTGATCGA 
ACTGCGGCACGCCTGCGCGCGCTGACCGCCGGACAAGGAGAGCATCCATGCGCTATTCCGACTTCACCCAAC 
GTATCGCCGGCGACGGCGCCGCTGCCTGGGACATCCACTATCGCGCGCTGGCGCGGGTCGAGCAGGGCGAGG 
AAATCCTCCTGCTGTCGGTCGGCGACCCCGACTTCGATACCCCGGCGCCCATCGTCCAGTCGGCCATCGACA 
GCCTGCTGGCCGGCAACACCCACTACGCCGACGTGCGCGGCAAGCGCGCCCTGCGCCAGCGCATCGCCGAAC 
GCCACCGACAGCGCAGCGGCCAGGCGGTGGACGCGGAGCAGGTGGTGGTGCTGGCCGGCGCCCAGTGCGCGC 
TGTACGCGGTGGTGCAGTGCCTGCTGA