ViewGene
Locus Name:
GeneID:
Location:

GeneID:6814
TrivialName: PA14_65600
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65600
PAO1 Ortholog Locus:
Sequence Length:2271
Protein Length:756
Start: 5845876
Stop: 5843607
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/03/06. PA14_65600 (GeneID 6814) and PA14_65610 (GeneID 6812) replaced by PA14_65605 (GeneID 7389). Homology: gb|AAG08349.1|AE004909_5 (AE004909) topoisomerase IV subunit A [Pseudomonas aeruginosa PAO1]
Identities = 114/114 (100%), Positives = 114/114 (100%)
Sequence:
ATGAGCGAATCCCTCGATCTGAGCCTGGAAGGGGTCGAACGCCGGTCGTTGGCCGAGTTCACCGAGCAGGCC
TATCTGAATTATTCCATGTACGTGATCATGGACCGCGCCCTGCCGCATATCGGCGACGGCCTGAAACCGGTG
CAGCGACGCATCGTCTACGCCATGAGCGAACTGGGGCTGGATGCCGATTCCAAGCACAAGAAGTCGGCGCGC
ACCGTCGGCGACGTGCTCGGCAAGTTCCACCCGCACGGCGACTCGGCCTGCTACGAGGCCATGGTGCTGATG
GCGCAGCCGTTCTCCTATCGCTATCCGCTGGTGGACGGCCAGGGCAACTGGGGGGGCGCCGGACGATCCCAA
GTCCTTCGCCGCCATGCGTTATACCGAGGCGCGCCTGTCGCGCTATTCCGAGGTGCTGCTCAGCGAACTGGG
CCAGGGTACCGTGGACTGGGTACCGAACTTCGACGGCACCCTCGACGAGCCGGCCGTGCTGCCGGCCCGCCT
GCCCAACCTGCTGCTCAACGGCACCACCGGCATCGCGGTGGGCATGGCCACCGACGTGCCGCCGCACAACCT
GCGGGAAGTCGCGTCGGCCTGCGTGCGCCTGCTCGACCAGCCGGGCGCGACGGTCGCCGAATTGTGCGAACA
CGTGCCGGGCCCGGACTTCCCCACCGAAGCCGAGATCATCACCCCGCGCGCCGACCTGCAGAAGGTCTACGA
GACCGGCCGCGGTTCGGTGCGCATGCGCGCGGTGTACCGCGTCGAGGACGGCGACATCGTCATCCACGCCCT
GCCGCACCAGGTGTCCGGTTCCAAGGTGCTGGAACAGATCGCCGGGCAGATGCAGGCCAAGAAGCTGCCGAT
GGTGGCCGACCTGCGCGACGAGTCGGACCACGAGAACCCGACCCGCATCGTCATCATCCCGCGTTCGAACCG
GGTCGATGTCGAAGAGCTGATGACCCATCTGTTCGCCACCACCGACCTGGAAACCAGCTACCGGGTCAACCT
GAACATCATCGGCCTCGACGGCAAGCCGCAGGTCAAGGACCTGCGCCAGTTGCTCTCGGAGTGGCTGCAGTT
CCGCATCGGCACCGTGCGTCGACGCCTGCAGTTCCGCCTGGACAAGGTCGAGCGCCGCCTGCATCTGCTGGA
TGGCTTGCTGATCGCCTTCCTCAACCTCGACGAGGTGATCCACATCATCCGCACCGAGGATCAGCCCAAGGC
GGTGCTGATGGAGCGCTTCGAACTCAGCGAGGTGCAGGCCGACTACATCCTCGACACCCGCCTGCGCCAGTT
GGCACGCCTGGAAGAGATGAAGATCCGCGGCGAGCAGGAAGAGTTGCTGAAGGAGCAGAAGCGCCTGCAGAC
CCTGCTCGGCAGCGAGGCCAAGCTGAAGAAGCTGGTGCGCGAGGAGCTGATCAAGGACGCCGAGACCTACGG
CGACGACCGCCGTTCGCCGATCGTCGCCCGCGCCGAGGCCCGCGCGCTGTCGGAAACCGAGCTGATGCCCAC
CGAACCGGTGACCGTGGTGCTCTCGGAAAAAGGCTGGGTGCGTTGCGCCAAGGGCCACGACATCGACGCCGC
CGGCCTCTCCTACAAGGCCGGCGACGGCTTCAAGGCCGCCGCGCCGGGACGCTCGAACCAGTATGCGGTGTT
CATCGACTCCACCGGGCGCAGCTACTCGCTGCCGGCCCACAGCCTGCCGTCCGCGCGAGGCCAGGGCGAGCC
ACTCAGCGGCCGGCTGACGCCTCCGCCGGGGGCCAGCTTCGAATGCGTGCTGCTGCCGGACGACGATGCGCT
GTTCGTGATCGCTTCCGACGCCGGCTATGGTTTCGTGGTCAAGGGCGAGGACCTGCAAGCCAAGAACAAGGC
CGGCAAGGCCCTGCTCAGCCTGCCCAACGGCTCCGCCGTGGTGGCGCCGCGCCCGGTGCGCGATGTGGAGCA
GGATTGGCTGGCGGCCGTGACGACCGAGGGCCGTCTGCTATTGTTCAAGGTCTCCGACCTGCCGCAGCTCGG
CAAGGGCAAGGGCAACAAGATCATCGGCATCCCCGGCGAACGCGTGGCCAGCCGCGAGGAGTACCTCACCGA
CCTGGCTGTTCTGCCAGCCGGGGCGACGTTGGTCCTGCAGGCTGGAAAGCGTACCCTGTCGCTCAAGGGCGA
CGACCTGGAACACTACAAGGGGGAGCGGGGCCGGCGAGGCAACAAGCTGCCGCGCGGTTTCCAGCGCGTCGA
CAGCCTGCTGGTGGATATTCCGCCACAGGATTGAAATGA
Translation:
MSESLDLSLEGVERRSLAEFTEQAYLNYSMYVIMDRALPHIGDGLKPVQRRIVYAMSELGLDADSKHKKSAR
TVGDVLGKFHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGGAGRSQVLRRHALYRGAPVALFRGAAQRTG
PGYRGLGTELRRHPRRAGRAAGPPAQPAAQRHHRHRGGHGHRRAAAQPAGSRVGLRAPARPAGRDGRRIVRT
RAGPGLPHRSRDHHPARRPAEGLRDRPRFGAHARGVPRRGRRHRHPRPAAPGVRFQGAGTDRRADAGQEAAD
GGRPARRVGPREPDPHRHHPAFEPGRCRRADDPSVRHHRPGNQLPGQPEHHRPRRQAAGQGPAPVALGVAAV
PHRHRASTPAVPPGQGRAPPASAGWLADRLPQPRRGDPHHPHRGSAQGGADGALRTQRGAGRLHPRHPPAPV
GTPGRDEDPRRAGRVAEGAEAPADPARQRGQAEEAGARGADQGRRDLRRRPPFADRRPRRGPRAVGNRADAH
RTGDRGALGKRLGALRQGPRHRRRRPLLQGRRRLQGRRAGTLEPVCGVHRLHRAQLLAAGPQPAVRARPGRA
TQRPADASAGGQLRMRAAAGRRCAVRDRFRRRLWFRGQGRGPASQEQGRQGPAQPAQRLRRGGAAPGARCGA
GLAGGRDDRGPSAIVQGLRPAAARQGQGQQDHRHPRRTRGQPRGVPHRPGCSASRGDVGPAGWKAYPVAQGR
RPGTLQGGAGPARQQAAARFPARRQPAGGYSATGLK*
AnnotationID:6815GeneID:6814
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:42
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:5.99.1.-
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAN70479.1|AE016689_7 (AE016792) DNA topoisomerase IV, A subunit [Pseudomonas putida
           KT2440]
          Length = 752

 Score =  225 bits (573), Expect = 9e-58
 Identities = 108/114 (94%), Positives = 113/114 (99%)

>gb|AAO58388.1| (AE016874) DNA topoisomerase IV, A subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 751

 Score =  223 bits (569), Expect = 3e-57
 Identities = 107/114 (93%), Positives = 112/114 (98%)

>gb|AAN56872.1|AE015822_4 (AE015822) DNA topoisomerase IV, A subunit [Shewanella oneidensis
           MR-1]
          Length = 758

 Score =  210 bits (535), Expect = 2e-53
 Identities = 99/114 (86%), Positives = 107/114 (93%)






 
Structural Features:






   
Genomic Context:






   
Comment:
Inactivated 2/03/06.  PA14_65600 (GeneID 6814) and PA14_65610 (GeneID 6812) 
replaced by PA14_65605 (GeneID 7389).


















    ReferenceID:13348




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30711




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35127




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6814_cdd.html


    		




 
URL:

















    ReferenceID:35886




  
Author/Investigator(s):
        Akasaka T, Onodera Y, Tanaka M, Sato K.





  
Title:
        Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV.




  
PubMed:
        10049263


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1999 Mar;43(3):530-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:Antimicrob Agents Chemother. 1999 Mar;43(3):530-6.









           
Homologs By Global Alignment 

           

       



     
Gene ID:6814
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
201927
                           dbj|BAA37152.1|
                           (AB003428) topoisomerase IV subunit [Pseudomonas aeruginosa]
                           924
                           28.35
                           34.30
                           338
                           566.0
     

     
     
8032
                           PA4964_tr
                           translation of PA4964
                           920
                           27.82
                           34.02
                           330
                           567.0
     

     
     
201926
                           gb|AAG08349.1|AE004909_5
                           (AE004909) topoisomerase IV subunit A [Pseudomonas aeruginosa PAO1]
                           920
                           27.82
                           34.02
                           330
                           567.0
     

     
     
201929
                           gb|AAO58388.1|
                           (AE016874) DNA topoisomerase IV, A subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           912
                           26.75
                           33.55
                           317
                           518.0
     

     
     
201948
                           gb|AAL22048.1|
                           (AE008846) DNA topoisomerase IV, subunit A [Salmonella typhimurium LT2]
                           914
                           26.25
                           33.80
                           320
                           466.0
     

     






      

          
GC ORFID: 49816How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5109558Stop: 5107288

          
Length: 2271


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4964 translation of PA4964Homolog Bit Score 234.0

          
Other Homologs:  t_PA4964 translation of PA4964 (BLASTP 234), psyr_15may02_Scaffold2_revised_gene3414 related to: gi|15600157|ref|NP_253651.1| (NC_002516) topoisomerase IV subunit A [Pseudomonas aeruginosa] pir||G83025 topoisomerase IV subunit A PA4964 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG08349.1|AE004909_5 (AE004909) topoisomerase IV subu-TRUNCATED- (BLASTP 223), t_PA3168 translation of PA3168 (BLASTP 133), psyr_15may02_Scaffold8_revised_gene7729 related to: gi|15598364|ref|NP_251858.1| (NC_002516) DNA gyrase subunit A [Pseudomonas aeruginosa] sp|P48372|GYRA_PSEAE DNA gyrase subunit A pir||H83248 DNA gyrase subunit A PA3168 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAA68625.1| (L29417) gyr-TRUNCATED- (BLASTP 130)

          
GC ORF Sequence

           
ATGAGCGAATCCCTCGATCTGAGCCTGGAAGGGGTCGAACGCCGGTCGTTGGCCGAGTTCACCGAGCAGGCC 
TATCTGAATTATTCCATGTACGTGATCATGGACCGCGCCCTGCCGCATATCGGCGACGGCCTGAAACCGGTG 
CAGCGACGCATCGTCTACGCCATGAGCGAACTGGGGCTGGATGCCGATTCCAAGCACAAGAAGTCGGCGCGC 
ACCGTCGGCGACGTGCTCGGCAAGTTCCACCCGCACGGCGACTCGGCCTGCTACGAGGCCATGGTGCTGATG 
GCGCAGCCGTTCTCCTATCGCTATCCGCTGGTGGACGGCCAGGGCAACTGGGGGGGCGCCGGACGATCCCAA 
GTCCTTCGCCGCCATGCGTTATACCGAGGCGCGCCTGTCGCGCTATTCCGAGGTGCTGCTCAGCGAACTGGG 
CCAGGGTACCGTGGACTGGGTACCGAACTTCGACGGCACCCTCGACGAGCCGGCCGTGCTGCCGGCCCGCCT 
GCCCAACCTGCTGCTCAACGGCACCACCGGCATCGCGGTGGGCATGGCCACCGACGTGCCGCCGCACAACCT 
GCGGGAAGTCGCGTCGGCCTGCGTGCGCCTGCTCGACCAGCCGGGCGCGACGGTCGCCGAATTGTGCGAACA 
CGTGCCGGGCCCGGACTTCCCCACCGAAGCCGAGATCATCACCCCGCGCGCCGACCTGCAGAAGGTCTACGA 
GACCGGCCGCGGTTCGGTGCGCATGCGCGCGGTGTACCGCGTCGAGGACGGCGACATCGTCATCCACGCCCT 
GCCGCACCAGGTGTCCGGTTCCAAGGTGCTGGAACAGATCGCCGGGCAGATGCAGGCCAAGAAGCTGCCGAT 
GGTGGCCGACCTGCGCGACGAGTCGGACCACGAGAACCCGACCCGCATCGTCATCATCCCGCGTTCGAACCG 
GGTCGATGTCGAAGAGCTGATGACCCATCTGTTCGCCACCACCGACCTGGAAACCAGCTACCGGGTCAACCT 
GAACATCATCGGCCTCGACGGCAAGCCGCAGGTCAAGGACCTGCGCCAGTTGCTCTCGGAGTGGCTGCAGTT 
CCGCATCGGCACCGTGCGTCGACGCCTGCAGTTCCGCCTGGACAAGGTCGAGCGCCGCCTGCATCTGCTGGA 
TGGCTTGCTGATCGCCTTCCTCAACCTCGACGAGGTGATCCACATCATCCGCACCGAGGATCAGCCCAAGGC 
GGTGCTGATGGAGCGCTTCGAACTCAGCGAGGTGCAGGCCGACTACATCCTCGACACCCGCCTGCGCCAGTT 
GGCACGCCTGGAAGAGATGAAGATCCGCGGCGAGCAGGAAGAGTTGCTGAAGGAGCAGAAGCGCCTGCAGAC 
CCTGCTCGGCAGCGAGGCCAAGCTGAAGAAGCTGGTGCGCGAGGAGCTGATCAAGGACGCCGAGACCTACGG 
CGACGACCGCCGTTCGCCGATCGTCGCCCGCGCCGAGGCCCGCGCGCTGTCGGAAACCGAGCTGATGCCCAC 
CGAACCGGTGACCGTGGTGCTCTCGGAAAAAGGCTGGGTGCGTTGCGCCAAGGGCCACGACATCGACGCCGC 
CGGCCTCTCCTACAAGGCCGGCGACGGCTTCAAGGCCGCCGCGCCGGGACGCTCGAACCAGTATGCGGTGTT 
CATCGACTCCACCGGGCGCAGCTACTCGCTGCCGGCCCACAGCCTGCCGTCCGCGCGAGGCCAGGGCGAGCC 
ACTCAGCGGCCGGCTGACGCCTCCGCCGGGGGCCAGCTTCGAATGCGTGCTGCTGCCGGACGACGATGCGCT 
GTTCGTGATCGCTTCCGACGCCGGCTATGGTTTCGTGGTCAAGGGCGAGGACCTGCAAGCCAAGAACAAGGC 
CGGCAAGGCCCTGCTCAGCCTGCCCAACGGCTCCGCCGTGGTGGCGCCGCGCCCGGTGCGCGATGTGGAGCA 
GGATTGGCTGGCGGCCGTGACGACCGAGGGCCGTCTGCTATTGTTCAAGGTCTCCGACCTGCCGCAGCTCGG 
CAAGGGCAAGGGCAACAAGATCATCGGCATCCCCGGCGAACGCGTGGCCAGCCGCGAGGAGTACCTCACCGA 
CCTGGCTGTTCTGCCAGCCGGGGCGACGTTGGTCCTGCAGGCTGGAAAGCGTACCCTGTCGCTCAAGGGCGA 
CGACCTGGAACACTACAAGGGGGAGCGGGGCCGGCGAGGCAACAAGCTGCCGCGCGGTTTCCAGCGCGTCGA 
CAGCCTGCTGGTGGATATTCCGCCACAGGATTGAAATGA