ViewGene
Locus Name:
GeneID:
Location:

GeneID:6812
TrivialName: PA14_65610
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65610
PAO1 Ortholog Locus:
Sequence Length:1893
Protein Length:630
Start: 5845504
Stop: 5843612
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Inactivated 2/03/06. PA14_65600 (GeneID 6814) and PA14_65610 (GeneID 6812) replaced by PA14_65605 (GeneID 7389). Homology: gb|AAG08349.1|AE004909_5 (AE004909) topoisomerase IV subunit A [Pseudomonas aeruginosa PAO1]
Identities = 630/630 (100%), Positives = 630/630 (100%)
Sequence:
ATGCGTTATACCGAGGCGCGCCTGTCGCGCTATTCCGAGGTGCTGCTCAGCGAACTGGGCCAGGGTACCGTG
GACTGGGTACCGAACTTCGACGGCACCCTCGACGAGCCGGCCGTGCTGCCGGCCCGCCTGCCCAACCTGCTG
CTCAACGGCACCACCGGCATCGCGGTGGGCATGGCCACCGACGTGCCGCCGCACAACCTGCGGGAAGTCGCG
TCGGCCTGCGTGCGCCTGCTCGACCAGCCGGGCGCGACGGTCGCCGAATTGTGCGAACACGTGCCGGGCCCG
GACTTCCCCACCGAAGCCGAGATCATCACCCCGCGCGCCGACCTGCAGAAGGTCTACGAGACCGGCCGCGGT
TCGGTGCGCATGCGCGCGGTGTACCGCGTCGAGGACGGCGACATCGTCATCCACGCCCTGCCGCACCAGGTG
TCCGGTTCCAAGGTGCTGGAACAGATCGCCGGGCAGATGCAGGCCAAGAAGCTGCCGATGGTGGCCGACCTG
CGCGACGAGTCGGACCACGAGAACCCGACCCGCATCGTCATCATCCCGCGTTCGAACCGGGTCGATGTCGAA
GAGCTGATGACCCATCTGTTCGCCACCACCGACCTGGAAACCAGCTACCGGGTCAACCTGAACATCATCGGC
CTCGACGGCAAGCCGCAGGTCAAGGACCTGCGCCAGTTGCTCTCGGAGTGGCTGCAGTTCCGCATCGGCACC
GTGCGTCGACGCCTGCAGTTCCGCCTGGACAAGGTCGAGCGCCGCCTGCATCTGCTGGATGGCTTGCTGATC
GCCTTCCTCAACCTCGACGAGGTGATCCACATCATCCGCACCGAGGATCAGCCCAAGGCGGTGCTGATGGAG
CGCTTCGAACTCAGCGAGGTGCAGGCCGACTACATCCTCGACACCCGCCTGCGCCAGTTGGCACGCCTGGAA
GAGATGAAGATCCGCGGCGAGCAGGAAGAGTTGCTGAAGGAGCAGAAGCGCCTGCAGACCCTGCTCGGCAGC
GAGGCCAAGCTGAAGAAGCTGGTGCGCGAGGAGCTGATCAAGGACGCCGAGACCTACGGCGACGACCGCCGT
TCGCCGATCGTCGCCCGCGCCGAGGCCCGCGCGCTGTCGGAAACCGAGCTGATGCCCACCGAACCGGTGACC
GTGGTGCTCTCGGAAAAAGGCTGGGTGCGTTGCGCCAAGGGCCACGACATCGACGCCGCCGGCCTCTCCTAC
AAGGCCGGCGACGGCTTCAAGGCCGCCGCGCCGGGACGCTCGAACCAGTATGCGGTGTTCATCGACTCCACC
GGGCGCAGCTACTCGCTGCCGGCCCACAGCCTGCCGTCCGCGCGAGGCCAGGGCGAGCCACTCAGCGGCCGG
CTGACGCCTCCGCCGGGGGCCAGCTTCGAATGCGTGCTGCTGCCGGACGACGATGCGCTGTTCGTGATCGCT
TCCGACGCCGGCTATGGTTTCGTGGTCAAGGGCGAGGACCTGCAAGCCAAGAACAAGGCCGGCAAGGCCCTG
CTCAGCCTGCCCAACGGCTCCGCCGTGGTGGCGCCGCGCCCGGTGCGCGATGTGGAGCAGGATTGGCTGGCG
GCCGTGACGACCGAGGGCCGTCTGCTATTGTTCAAGGTCTCCGACCTGCCGCAGCTCGGCAAGGGCAAGGGC
AACAAGATCATCGGCATCCCCGGCGAACGCGTGGCCAGCCGCGAGGAGTACCTCACCGACCTGGCTGTTCTG
CCAGCCGGGGCGACGTTGGTCCTGCAGGCTGGAAAGCGTACCCTGTCGCTCAAGGGCGACGACCTGGAACAC
TACAAGGGGGAGCGGGGCCGGCGAGGCAACAAGCTGCCGCGCGGTTTCCAGCGCGTCGACAGCCTGCTGGTG
GATATTCCGCCACAGGATTGA
Translation:
MRYTEARLSRYSEVLLSELGQGTVDWVPNFDGTLDEPAVLPARLPNLLLNGTTGIAVGMATDVPPHNLREVA
SACVRLLDQPGATVAELCEHVPGPDFPTEAEIITPRADLQKVYETGRGSVRMRAVYRVEDGDIVIHALPHQV
SGSKVLEQIAGQMQAKKLPMVADLRDESDHENPTRIVIIPRSNRVDVEELMTHLFATTDLETSYRVNLNIIG
LDGKPQVKDLRQLLSEWLQFRIGTVRRRLQFRLDKVERRLHLLDGLLIAFLNLDEVIHIIRTEDQPKAVLME
RFELSEVQADYILDTRLRQLARLEEMKIRGEQEELLKEQKRLQTLLGSEAKLKKLVREELIKDAETYGDDRR
SPIVARAEARALSETELMPTEPVTVVLSEKGWVRCAKGHDIDAAGLSYKAGDGFKAAAPGRSNQYAVFIDST
GRSYSLPAHSLPSARGQGEPLSGRLTPPPGASFECVLLPDDDALFVIASDAGYGFVVKGEDLQAKNKAGKAL
LSLPNGSAVVAPRPVRDVEQDWLAAVTTEGRLLLFKVSDLPQLGKGKGNKIIGIPGERVASREEYLTDLAVL
PAGATLVLQAGKRTLSLKGDDLEHYKGERGRRGNKLPRGFQRVDSLLVDIPPQD*
AnnotationID:6813GeneID:6812
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:11
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0188,pfam00521
Secondary Functional Category(ies):
EC Number:5.99.1.-
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAN70479.1|AE016689_7 (AE016792) DNA topoisomerase IV, A subunit [Pseudomonas putida
           KT2440]
          Length = 752

 Score = 1095 bits (2832), Expect = 0.0
 Identities = 542/627 (86%), Positives = 592/627 (94%)

>gb|AAO58388.1| (AE016874) DNA topoisomerase IV, A subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 751

 Score = 1074 bits (2778), Expect = 0.0
 Identities = 536/625 (85%), Positives = 584/625 (93%)

>dbj|BAC58694.1| (AP005074) DNA topoisomerase IV [Vibrio parahaemolyticus]
          Length = 761

 Score =  833 bits (2152), Expect = 0.0
 Identities = 408/626 (65%), Positives = 516/626 (82%)






 
Structural Features:
COG0188, GyrA, Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit 
[DNA replication, recombination, and repair]
CD-Length = 804 residues,  77.9% aligned
Score =  579 bits (1494), Expect = 4e-166
Identities = 255/639 (39%), Positives = 381/639 (59%), Gaps = 28/639 (4%)

pfam00521, DNA_topoisoIV, DNA gyrase/topoisomerase IV, subunit A.
CD-Length = 446 residues,  80.3% aligned
Score =  410 bits (1055), Expect = 3e-115
Identities = 171/362 (47%), Positives = 235/362 (64%), Gaps = 10/362 (2%)






   
Genomic Context:






   
Comment:
Inactivated 2/03/06.  PA14_65600 (GeneID 6814) and PA14_65610 (GeneID 6812) 
replaced by PA14_65605 (GeneID 7389).


















    ReferenceID:13345




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30707




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35128




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6812_cdd.html


    		




 
URL:

















    ReferenceID:35887




  
Author/Investigator(s):
        Akasaka T, Onodera Y, Tanaka M, Sato K.





  
Title:
        Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV.




  
PubMed:
        10049263


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1999 Mar;43(3):530-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10049263









           
Homologs By Global Alignment 

           

       



     
Gene ID:6812
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8031
                           PA4964_tr
                           translation of PA4964
                           754
                           83.55
                           83.55
                           124
                           3198.0
     

     
     
201774
                           gb|AAG08349.1|AE004909_5
                           (AE004909) topoisomerase IV subunit A [Pseudomonas aeruginosa PAO1]
                           754
                           83.55
                           83.55
                           124
                           3198.0
     

     
     
201775
                           dbj|BAA37152.1|
                           (AB003428) topoisomerase IV subunit [Pseudomonas aeruginosa]
                           754
                           83.28
                           83.28
                           124
                           3187.0
     

     
     
201776
                           gb|AAN70479.1|AE016689_7
                           (AE016792) DNA topoisomerase IV, A subunit [Pseudomonas putida KT2440]
                           754
                           71.88
                           78.51
                           126
                           2831.0
     

     
     
201777
                           gb|AAO58388.1|
                           (AE016874) DNA topoisomerase IV, A subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           754
                           71.08
                           77.45
                           127
                           2774.0
     

     






      

          
GC ORFID: 49814How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5109185Stop: 5107293

          
Length: 1893


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4964 translation of PA4964Homolog Bit Score 1236.0

          
Other Homologs:  t_PA4964 translation of PA4964 (BLASTP 1236), psyr_15may02_Scaffold2_revised_gene3414 related to: gi|15600157|ref|NP_253651.1| (NC_002516) topoisomerase IV subunit A [Pseudomonas aeruginosa] pir||G83025 topoisomerase IV subunit A PA4964 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG08349.1|AE004909_5 (AE004909) topoisomerase IV subu-TRUNCATED- (BLASTP 1070)

          
GC ORF Sequence

           
ATGCGTTATACCGAGGCGCGCCTGTCGCGCTATTCCGAGGTGCTGCTCAGCGAACTGGGCCAGGGTACCGTG 
GACTGGGTACCGAACTTCGACGGCACCCTCGACGAGCCGGCCGTGCTGCCGGCCCGCCTGCCCAACCTGCTG 
CTCAACGGCACCACCGGCATCGCGGTGGGCATGGCCACCGACGTGCCGCCGCACAACCTGCGGGAAGTCGCG 
TCGGCCTGCGTGCGCCTGCTCGACCAGCCGGGCGCGACGGTCGCCGAATTGTGCGAACACGTGCCGGGCCCG 
GACTTCCCCACCGAAGCCGAGATCATCACCCCGCGCGCCGACCTGCAGAAGGTCTACGAGACCGGCCGCGGT 
TCGGTGCGCATGCGCGCGGTGTACCGCGTCGAGGACGGCGACATCGTCATCCACGCCCTGCCGCACCAGGTG 
TCCGGTTCCAAGGTGCTGGAACAGATCGCCGGGCAGATGCAGGCCAAGAAGCTGCCGATGGTGGCCGACCTG 
CGCGACGAGTCGGACCACGAGAACCCGACCCGCATCGTCATCATCCCGCGTTCGAACCGGGTCGATGTCGAA 
GAGCTGATGACCCATCTGTTCGCCACCACCGACCTGGAAACCAGCTACCGGGTCAACCTGAACATCATCGGC 
CTCGACGGCAAGCCGCAGGTCAAGGACCTGCGCCAGTTGCTCTCGGAGTGGCTGCAGTTCCGCATCGGCACC 
GTGCGTCGACGCCTGCAGTTCCGCCTGGACAAGGTCGAGCGCCGCCTGCATCTGCTGGATGGCTTGCTGATC 
GCCTTCCTCAACCTCGACGAGGTGATCCACATCATCCGCACCGAGGATCAGCCCAAGGCGGTGCTGATGGAG 
CGCTTCGAACTCAGCGAGGTGCAGGCCGACTACATCCTCGACACCCGCCTGCGCCAGTTGGCACGCCTGGAA 
GAGATGAAGATCCGCGGCGAGCAGGAAGAGTTGCTGAAGGAGCAGAAGCGCCTGCAGACCCTGCTCGGCAGC 
GAGGCCAAGCTGAAGAAGCTGGTGCGCGAGGAGCTGATCAAGGACGCCGAGACCTACGGCGACGACCGCCGT 
TCGCCGATCGTCGCCCGCGCCGAGGCCCGCGCGCTGTCGGAAACCGAGCTGATGCCCACCGAACCGGTGACC 
GTGGTGCTCTCGGAAAAAGGCTGGGTGCGTTGCGCCAAGGGCCACGACATCGACGCCGCCGGCCTCTCCTAC 
AAGGCCGGCGACGGCTTCAAGGCCGCCGCGCCGGGACGCTCGAACCAGTATGCGGTGTTCATCGACTCCACC 
GGGCGCAGCTACTCGCTGCCGGCCCACAGCCTGCCGTCCGCGCGAGGCCAGGGCGAGCCACTCAGCGGCCGG 
CTGACGCCTCCGCCGGGGGCCAGCTTCGAATGCGTGCTGCTGCCGGACGACGATGCGCTGTTCGTGATCGCT 
TCCGACGCCGGCTATGGTTTCGTGGTCAAGGGCGAGGACCTGCAAGCCAAGAACAAGGCCGGCAAGGCCCTG 
CTCAGCCTGCCCAACGGCTCCGCCGTGGTGGCGCCGCGCCCGGTGCGCGATGTGGAGCAGGATTGGCTGGCG 
GCCGTGACGACCGAGGGCCGTCTGCTATTGTTCAAGGTCTCCGACCTGCCGCAGCTCGGCAAGGGCAAGGGC 
AACAAGATCATCGGCATCCCCGGCGAACGCGTGGCCAGCCGCGAGGAGTACCTCACCGACCTGGCTGTTCTG 
CCAGCCGGGGCGACGTTGGTCCTGCAGGCTGGAAAGCGTACCCTGTCGCTCAAGGGCGACGACCTGGAACAC 
TACAAGGGGGAGCGGGGCCGGCGAGGCAACAAGCTGCCGCGCGGTTTCCAGCGCGTCGACAGCCTGCTGGTG 
GATATTCCGCCACAGGATTGA