ViewGene
Locus Name:
GeneID:
Location:

GeneID:6705
TrivialName: PA14_60440
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60440
PAO1 Ortholog Locus:
Sequence Length:1221
Protein Length:406
Start: 5388354
Stop: 5387134
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Inactivated 9-19-05 to correct frame-shift error due to problem with base call. Replaced with GeneID: 7356
Sequence:
ATGAAATTCGTCGATGAAGTATCAATCCACGTGAAAGCCGGTGACGGCGGCAATGGCCTCATGAGCTTTCGT
CGCGAGAAGTTCATCGAAAAGGGCGGTCCCAACGGCGGCGACGGTGGCGATGGCGGTTCCATCTACCTGGAA
GCCGACGTCAACCTGAACACCCTGGTCGACTATCGCTACACCCGCCGTTTCGATGCCCAGCGCGGCGAGAAC
GGCGGCAGCAAGGACTGCACCGGGGCCAAGGGCGACGACCTGATCCTGCCGGTGCCGGTCGGTACTACCGTG
ATCGACGCCAATACCCAGGAGATCATCGGCGACCTGACCGAGCCCGGTCAGCGGCTGATGGTCGCGCAGGGC
GGCTGGCATGGCCTCGGTAACACCCGTTTCAAATCCAGTACCAATCGGGCGCCGCGCCAGACCACCCCGGGC
AAGCCGGGCGAGGCGCGCGACCTCAAGCTGGAACTGAAGGTGCTGGCCGACGTCGGCCTGCTGGGACTGCCG
AATGCCGGCAAGAGCACTTTCATCCGCGCAGTGTCCGCGGCCAAGCCGAAGGTGGCCGACTATCCGTTCACC
ACACTGGTGCCGAACCTCGGCGTGGTCAGCGTCGGTCGCTACAAGAGCTTCGTGGTCGCCGACATTCCCGGC
CTGATCGAAGGTGCCGCCGAAGGCGCCGGGCTGGGCATCCGCTTCCTCAAGCATCTGGCGCGGACCCGTATC
CTCCTGCATCTGGTGGACATGGCGCCGCTGGACGAAAGCGATCCGGCCGATGCCGCCGAGGTCATCGTTCGC
GAGCTGGGTCGCTTCAGTCCGGCGCTGACCGAGCGCGAGCGCTGGCTGGTGCTGAACAAGATGGACCAGATC
CTCGATCCGGCCGAGCGCGAGGCGCGCAAGCAGGCGGTGATCGAGCGGTTGGGCTGGGAAGGCCCGGTCTAT
GTGATCTCCGCCCTGGAGCGCGATGGCACCGAGGCGCTCAGCCAGGACATCATGCGCTATCTCGACGAGCGT
ACCCTGCGCCTGGAAGAAGATCCGCAGTATGCCGAGGAGCTGGCCGAACTCGACCGGCGTATCGAGGACGAG
GCGCGGGCCCGTCTGCAGGCCCTGGACGATGCGCGCGCGCTGCGGCGTTCGGGGCTGAAGAACGCTGGTGCG
GTGGATGACGATGATTTCGATGATGAGGAAGACGACGGCGACGGTCCGGAGATCTTCTACGTTCCCTGA
Translation:
MKFVDEVSIHVKAGDGGNGLMSFRREKFIEKGGPNGGDGGDGGSIYLEADVNLNTLVDYRYTRRFDAQRGEN
GGSKDCTGAKGDDLILPVPVGTTVIDANTQEIIGDLTEPGQRLMVAQGGWHGLGNTRFKSSTNRAPRQTTPG
KPGEARDLKLELKVLADVGLLGLPNAGKSTFIRAVSAAKPKVADYPFTTLVPNLGVVSVGRYKSFVVADIPG
LIEGAAEGAGLGIRFLKHLARTRILLHLVDMAPLDESDPADAAEVIVRELGRFSPALTERERWLVLNKMDQI
LDPAEREARKQAVIERLGWEGPVYVISALERDGTEALSQDIMRYLDERTLRLEEDPQYAEELAELDRRIEDE
ARARLQALDDARALRRSGLKNAGAVDDDDFDDEEDDGDGPEIFYVP*
AnnotationID:6706GeneID:6705
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 18:06:30
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0536,KOG1489
Secondary Functional Category(ies):
EC Number:
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAO54341.1| (AE016858) GTP-binding protein, GTP1/Obg family [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 407

 Score =  463 bits (1191), Expect = e-130
 Identities = 232/291 (79%), Positives = 266/291 (91%), Gaps = 2/291 (0%)

>emb|CAD07818.1| (AL627278) probable GTP-binding protein [Salmonella enterica subsp.
           enterica serovar Typhi]
          Length = 390

 Score =  302 bits (774), Expect = 1e-81
 Identities = 160/289 (55%), Positives = 205/289 (70%), Gaps = 16/289 (5%)






 
Structural Features:
COG0536, Obg, Predicted GTPase
CD-Length = 369 residues, only  66.1% aligned
Score =  334 bits (859), Expect = 1e-92
Identities = 143/246 (58%), Positives = 181/246 (73%), Gaps = 2/246 (0%)

KOG1489, Predicted GTP-binding protein (ODN superfamily) 
CD-Length = 366 residues, only  56.3% aligned
Score =  200 bits (511), Expect = 3e-52
Identities = 88/210 (41%), Positives = 127/210 (60%), Gaps = 4/210 (1%)






   
Genomic Context:






   
Comment:
nactivated 9-19-05 to correct frame-shift error due to problem with base call. Replaced with GeneID: 7356


















    ReferenceID:13161




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30498




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31647




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6705_cdd.html


    		




 
URL:

















    ReferenceID:33102




  
Author/Investigator(s):
        Dutkiewicz R, Slominska M, Wegrzyn G, Czyz A.





  
Title:
        Overexpression of the cgtA (yhbZ, obgE) gene, coding for an essential GTP-binding protein, impairs the regulation of chromosomal functions in Escherichia coli.




  
PubMed:
        12402086


MedLine:
        




    
Source:
Curr Microbiol. 2002 Dec;45(6):440-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12402086

















    ReferenceID:33103




  
Author/Investigator(s):
        Slominska M, Konopa G, Wegrzyn G, Czyz A.





  
Title:
        Impaired chromosome partitioning and synchronization of DNA replication initiation in an insertional mutant in the Vibrio harveyi cgtA gene coding for a common GTP-binding protein.




  
PubMed:
        11879184


MedLine:
        




    
Source:
Biochem J. 2002 Mar 15;362(Pt 3):579-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11879184

















    ReferenceID:33104




  
Author/Investigator(s):
        Mittenhuber G.





  
Title:
        Comparative genomics of prokaryotic GTP-binding proteins (the Era, Obg, EngA, ThdF (TrmE), YchF and YihA families) and their relationship to eukaryotic GTP-binding proteins (the DRG, ARF, RAB, RAN, RAS and RHO families).




  
PubMed:
        11200227


MedLine:
        




    
Source:
J Mol Microbiol Biotechnol. 2001 Jan;3(1):21-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11200227

















    ReferenceID:33105




  
Author/Investigator(s):
        Okamoto S, Itoh M, Ochi K.





  
Title:
        Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation.




  
PubMed:
        8981995


MedLine:
        




    
Source:
J Bacteriol. 1997 Jan;179(1):170-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8981995

















    ReferenceID:33106




  
Author/Investigator(s):
        Vidwans SJ, Ireton K, Grossman AD.





  
Title:
        Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis.




  
PubMed:
        7768831


MedLine:
        




    
Source:
J Bacteriol. 1995 Jun;177(11):3308-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7768831

















    ReferenceID:33107




  
Author/Investigator(s):
        Welsh KM, Trach KA, Folger C, Hoch JA.





  
Title:
        Biochemical characterization of the essential GTP-binding protein Obg of Bacillus subtilis.




  
PubMed:
        7961487


MedLine:
        




    
Source:
J Bacteriol. 1994 Dec;176(23):7161-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7961487

















    ReferenceID:33108




  
Author/Investigator(s):
        Trach K, Hoch JA.





  
Title:
        The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.




  
PubMed:
        2537815


MedLine:
        




    
Source:
J Bacteriol. 1989 Mar;171(3):1362-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2537815









           
Homologs By Global Alignment 

           

       



     
Gene ID:6705
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8027
                           PA4566_tr
                           translation of PA4566
                           406
                           71.67
                           71.67
                           115
                           1480.0
     

     
     
201515
                           gb|AAG07954.1|AE004870_5
                           (AE004870) GTP-binding protein Obg [Pseudomonas aeruginosa PAO1]
                           406
                           71.67
                           71.67
                           115
                           1480.0
     

     
     
201516
                           gb|AAO54341.1|
                           (AE016858) GTP-binding protein, GTP1/Obg family [Pseudomonas syringae pv. tomato str. DC3000]
                           408
                           56.86
                           65.19
                           118
                           1193.0
     

     
     
201517
                           gb|AAN66315.1|AE016260_1
                           (AE016776) GTP-binding protein, GTP1/Obg family [Pseudomonas putida KT2440]
                           409
                           56.72
                           63.08
                           119
                           1182.0
     

     
     
201535
                           gb|AAC22533.1|
                           (U32769) GTP-binding protein (yhbZ) [Haemophilus influenzae Rd] [Haemophilus influenzae Rd KW20]
                           410
                           40.73
                           48.78
                           139
                           763.0
     

     






      

          
GC ORFID: 49707How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 4651688Stop: 4650813

          
Length: 876


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4566 translation of PA4566Homolog Bit Score 574.0

          
Other Homologs:  t_PA4566 translation of PA4566 (BLASTP 574), psyr_15may02_Scaffold2_revised_gene3528 related to: gi|15599762|ref|NP_253256.1| (NC_002516) GTP-binding protein Obg [Pseudomonas aeruginosa] pir||H83074 GTP-binding protein Obg PA4566 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG07954.1|AE004870_5 (AE004870) GTP-binding protein Obg [Ps-TRUNCATED- (BLASTP 462)

          
GC ORF Sequence

           
ATGGTCGCGCAGGGCGGCTGGCATGGCCTCGGTAACACCCGTTTCAAATCCAGTACCAATCGGGCGCCGCGC 
CAGACCACCCCGGGCAAGCCGGGCGAGGCGCGCGACCTCAAGCTGGAACTGAAGGTGCTGGCCGACGTCGGC 
CTGCTGGGACTGCCGAATGCCGGCAAGAGCACTTTCATCCGCGCAGTGTCCGCGGCCAAGCCGAAGGTGGCC 
GACTATCCGTTCACCACACTGGTGCCGAACCTCGGCGTGGTCAGCGTCGGTCGCTACAAGAGCTTCGTGGTC 
GCCGACATTCCCGGCCTGATCGAAGGTGCCGCCGAAGGCGCCGGGCTGGGCATCCGCTTCCTCAAGCATCTG 
GCGCGGACCCGTATCCTCCTGCATCTGGTGGACATGGCGCCGCTGGACGAAAGCGATCCGGCCGATGCCGCC 
GAGGTCATCGTTCGCGAGCTGGGTCGCTTCAGTCCGGCGCTGACCGAGCGCGAGCGCTGGCTGGTGCTGAAC 
AAGATGGACCAGATCCTCGATCCGGCCGAGCGCGAGGCGCGCAAGCAGGCGGTGATCGAGCGGTTGGGCTGG 
GAAGGCCCGGTCTATGTGATCTCCGCCCTGGAGCGCGATGGCACCGAGGCGCTCAGCCAGGACATCATGCGC 
TATCTCGACGAGCGTACCCTGCGCCTGGAAGAAGATCCGCAGTATGCCGAGGAGCTGGCCGAACTCGACCGG 
CGTATCGAGGACGAGGCGCGGGCCCGTCTGCAGGCCCTGGACGATGCGCGCGCGCTGCGGCGTTCGGGGCTG 
AAGAACGCTGGTGCGGTGGATGACGATGATTTCGATGATGAGGAAGACGACGGCGACGGTCCGGAGATCTTC 
TACGTTCCCTGA