ViewGene
Locus Name:
GeneID:
Location:

GeneID:6455
TrivialName: PA14_15390
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_15390
PAO1 Ortholog Locus:
Sequence Length:738
Protein Length:245
Start: 1304193
Stop: 1304930
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
INACTIVATED, replaced by PA14_15380, GeneID 7265. Homology: gb|AAK97757.1| (AY043299) RepA [Aeromonas salmonicida]
Identities = 197/239 (82%), Positives = 219/239 (91%), Gaps = 1/239 (0%)
Sequence:
ATGAGCATCGCATGCAGCGTTCCGGGTGGCGACCTCGTGGGCCTTGCCCCCGCACATTCCGGGCGCGTGGTC
TATCTGGCCGGTGAAGACCCGCCGCCCGCGCTTGTGCGGCGCGTTCACGCCATCGGCCAGCACCTCGGGCAC
GCAGCTCGCCAAGCCATCGCAGAGAACTTGGTGCTTGAGCCGATCATGGGCAAGCGCATGGACATCATGAAC
AAGGCTCACCTGCGCCGCGTCATTGAGGACGGTGCCGGGGCGAGGCTGATTGTGCTGGATACCCTGAGCCGT
ATTCACGCCCTCGATGAGAACAGCAACGGCAACATGGCCCACCTCGTGGCCGTGTTGGAGCACGTTGCGGCC
ACCACGGGCGCATCCGTGCTCTACCTACACCACGTCAGCAAGGGAAGCGCCCGCGAAGGGCAAACCGACCAG
CAGCAGGCTGCGCGTGGCGCATCGGCGTTGATCGACAACGCCAGGTGGTGTGGTTACGTCGCTCGCATGACC
GAGGATGAGGCAAAGCGCCTCAGCGACCGCCCCCACGACCGGCAGCCCATCGGTAATGAGCGCCGCAGCTAC
TTCGTGCGTTTCGGCGTCAGCAAGCAGAACTACGACGCCACGCCGCTTGATCGCTGGTACATGCGGCACTCC
GGCGGCGTACTGGTGCCAGTGGAACTGCATGAGGCCCGCAGGAGTGAGGAGAAGGGATGCGATGGCCGGAGG
CGAAGCGATGGCTTTTGA
Translation:
MSIACSVPGGDLVGLAPAHSGRVVYLAGEDPPPALVRRVHAIGQHLGHAARQAIAENLVLEPIMGKRMDIMN
KAHLRRVIEDGAGARLIVLDTLSRIHALDENSNGNMAHLVAVLEHVAATTGASVLYLHHVSKGSAREGQTDQ
QQAARGASALIDNARWCGYVARMTEDEAKRLSDRPHDRQPIGNERRSYFVRFGVSKQNYDATPLDRWYMRHS
GGVLVPVELHEARRSEEKGCDGRRRSDGF*
AnnotationID:6456GeneID:6455
AnnotatorUID: diggins
Modification Date/Time: 2006-02-09 08:08:21
Gene Name:
Confidence Code:3
GeneProduct:putative replicative helicase, RepA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd01125
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|15983526|ref|NP_387463.1|  RepA [Aeromonas salmonicida]
gi|15822667|gb|AAK97757.1|  RepA [Aeromonas salmonicida]
     Length=292
     Score =  397 bits (1019),  Expect = 2e-109
     Identities = 197/239 (82%), Positives = 219/239 (91%), Gaps = 1/239 (0%)

gi|40850641|gb|AAR96040.1|  RepA [Chlamydia suis]
     Length=347
     Score =  397 bits (1019),  Expect = 2e-109
     Identities = 197/239 (82%), Positives = 219/239 (91%), Gaps = 1/239 (0%)

gi|154647|gb|AAA27381.1|  replication protein A
     Length=290
     Score =  391 bits (1004),  Expect = 1e-107
     Identities = 195/243 (80%), Positives = 217/243 (89%), Gaps = 1/243 (0%)






 
Structural Features:
cd01125, repA, Hexameric Replicative Helicase RepA. RepA is encoded by a plasmid, which is found in most 
Gram negative bacteria. RepA is a 5'-3' DNA helicase which can utilize ATP, GTP and CTP to a lesser extent.
             CD-Length = 239 residues,  90.8% aligned
             Score =  169 bits (429), Expect = 3e-43

COG3598, RepA, RecA-family ATPase [DNA replication, recombination, and repair].
             CD-Length = 402 residues, only  37.1% aligned
             Score = 55.8 bits (134), Expect = 5e-09






   
Genomic Context:






   
Comment:


















    ReferenceID:12719




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:16955




  
Author/Investigator(s):
        L'Abee-Lund,T.M. and Sorum,H.





  
Title:
         A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas
            salmonicida




  
PubMed:
        12151232


MedLine:
        22146887




    
Source:
JOURNAL   Plasmid 47 (3), 172-181 (2002)







    
Reference Type:Journal 







    
Data:


    		




 
URL:

















    ReferenceID:16956




  
Author/Investigator(s):
        Dorrington,R.A. and Rawlings,D.E.





  
Title:
        Characterization of the minimum replicon of the broad-host-range
            plasmid pTF-FC2 and similarity between pTF-FC2 and the IncQ
            plasmids




  
PubMed:
        2120189


MedLine:
        91008941




    
Source:
J. Bacteriol. 172 (10), 5697-5705 (1990)







    
Reference Type:Journal 







    
Data:


    		




 
URL:

















    ReferenceID:30015




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6455
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
199873
                           gb|AAK97757.1|
                           (AY043299) RepA [Aeromonas salmonicida]
                           294
                           67.00
                           74.82
                           51
                           1018.0
     

     
     
199875
                           gb|AAA27381.1|
                           (M73777) replication protein A [Plasmid pTF-FC2]
                           292
                           66.78
                           74.31
                           49
                           1002.0
     

     
     
199874
                           gb|AAK97750.1|
                           (AY043298) RepA [Aeromonas salmonicida subsp. salmonicida]
                           294
                           66.66
                           74.48
                           51
                           1010.0
     

     
     
199876
                           gb|AAK56915.1|AF325537_4
                           (AF325537) helicase-like protein [Acidithiobacillus caldus]
                           292
                           61.64
                           70.54
                           48
                           923.0
     

     
     
199878
                           emb|CAC05682.1|
                           (AJ293027) putative DNA helicase [uncultured eubacterium pIE1115]
                           296
                           36.48
                           51.68
                           68
                           518.0
     

     






      

          
GC ORFID: 49457How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 3649744Stop: 3649007

          
Length: 738


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: Homolog Bit Score 

          
Other Homologs:  

          
GC ORF Sequence

           
ATGAGCATCGCATGCAGCGTTCCGGGTGGCGACCTCGTGGGCCTTGCCCCCGCACATTCCGGGCGCGTGGTC 
TATCTGGCCGGTGAAGACCCGCCGCCCGCGCTTGTGCGGCGCGTTCACGCCATCGGCCAGCACCTCGGGCAC 
GCAGCTCGCCAAGCCATCGCAGAGAACTTGGTGCTTGAGCCGATCATGGGCAAGCGCATGGACATCATGAAC 
AAGGCTCACCTGCGCCGCGTCATTGAGGACGGTGCCGGGGCGAGGCTGATTGTGCTGGATACCCTGAGCCGT 
ATTCACGCCCTCGATGAGAACAGCAACGGCAACATGGCCCACCTCGTGGCCGTGTTGGAGCACGTTGCGGCC 
ACCACGGGCGCATCCGTGCTCTACCTACACCACGTCAGCAAGGGAAGCGCCCGCGAAGGGCAAACCGACCAG 
CAGCAGGCTGCGCGTGGCGCATCGGCGTTGATCGACAACGCCAGGTGGTGTGGTTACGTCGCTCGCATGACC 
GAGGATGAGGCAAAGCGCCTCAGCGACCGCCCCCACGACCGGCAGCCCATCGGTAATGAGCGCCGCAGCTAC 
TTCGTGCGTTTCGGCGTCAGCAAGCAGAACTACGACGCCACGCCGCTTGATCGCTGGTACATGCGGCACTCC 
GGCGGCGTACTGGTGCCAGTGGAACTGCATGAGGCCCGCAGGAGTGAGGAGAAGGGATGCGATGGCCGGAGG 
CGAAGCGATGGCTTTTGA