ViewGene
Locus Name:
GeneID:
Location:

GeneID:6377
TrivialName: PA14_18290
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18290
PAO1 Ortholog Locus:
Sequence Length:1350
Protein Length:449
Start: 1567943
Stop: 1569292
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Inactivated 2/01/06. PA14_18270 (GeneID 6379) and PA14_18290 (GeneID 6377) replaced by PA14_18275 (GeneID 7380). Homology: gb|AAG06948.1|AE004777_1 (AE004777) phosphotransferase system, fructose-specific IIBC component [Pseudomonas aeruginosa PAO1]
Identities = 449/449 (100%), Positives = 449/449 (100%)
Sequence:
ATGGCCGCCGAGGCGTTGCAGCTCGCGGCGGGGCAACTGGGCTTCGCGTTGCAGGTCGAGACCCAGGGCTCG
GTCGGCGCGCGCAACCCGCTCGACCCGGCGGACATCGCCGCCGCCGACGTGGTCCTGCTGGCCGCCGACATC
GACGTGGACACGGCCCGTTTCGCCGGCAAGAAGATCTACCGTTGCGGCACCGGCGTGGCCCTCAAGCAGGCC
CGCGCGACGCTCGAGCGGGCGCTGGCGGAAGGGCAGGTCGAGAGCGCGGGCGCAGCCAGCGCGGTGGTCGCC
AGGGACGAGAAGCGCGGGGTGTACAAGCACCTGCTGACCGGGGTGTCGTTCATGTTGCCGATGGTGGTCGCG
GGCGGCCTGCTGATCGCCCTGTCGCTGGCCTTCGGCATCGATGCCTACAAGCAGCCGGGCAGCCTGGCGGCG
GTGCTGAGGACGGTCGGCGACACGGCTTTCGTCCTGATGGTGCCGATGCTCGCCGGCTACATCGCCTATTCC
ATCGCCGACCGCCCGGGCCTGGCGCCGGGCATGCTCGGCGGCCTGCTCGCCGGGACCCTCGGCGCCGGGTTC
ATCGGCGGGATAGTCGCCGGCTTCATCGCCGGCTATGCGGCGCGGGCCATCAGCCACGGGCTGAGGCTGCCG
GCGAGCCTGGAAGCGCTGAAGCCGATCCTGGTCATCCCGTTGCTGGCCAGCCTGGTCACCGGCCTGCTGATG
CTCTACGTGGTCGGCAAGCCGGTGGCCGGGATGCTCGCGGCACTGACCGGGTTCCTCGACGGCATGGGTACC
TCCAATGCCATCCTCCTCGGCCTGCTGCTCGGCGGGATGATGTGCGTCGACCTCGGCGGACCGGTCAACAAG
GCCGCCTACGCCTTTTCCGTCGGCCTGCTCTCGTCGCACAGCTACGCGCCGATGGCGGCGGTGATGGCGGCG
GGCATGGTGCCGCCGATCGGCATGGGCCTGGCGACCCTGCTGGCGCGGCGCAAGTTCGCCGAGAGCGAGCGG
CAGGCCGGCAAGGCAGCCTCGGTGCTGGGGCTGTGCTTCATCTCCGAAGGCGCGATCCCGTTCGCCGCCAAG
GACCCGTTGCGGGTGATCCCGGCGAGCATCGCCGGCGGCGCGCTGACCGGCGCCCTGTCGATGTATTTCGGC
TGCAAGTTGCAGGCGCCTCACGGCGGGTTGTTCGTGATGCTGGTGCCCAACGCGATCAACCACGCCCTGGCG
TACCTGCTGGCGATCGTCGCCGGCAGCCTGCTTACCGGGCTGCTCTACGCCGTGCTCAAGCGCGGCGCCGAG
CCGGGACTGGCGTTGGGGACCTCGTCCTCGCGGGAGCCGGCGGCTGGCGGATAG
Translation:
MAAEALQLAAGQLGFALQVETQGSVGARNPLDPADIAAADVVLLAADIDVDTARFAGKKIYRCGTGVALKQA
RATLERALAEGQVESAGAASAVVARDEKRGVYKHLLTGVSFMLPMVVAGGLLIALSLAFGIDAYKQPGSLAA
VLRTVGDTAFVLMVPMLAGYIAYSIADRPGLAPGMLGGLLAGTLGAGFIGGIVAGFIAGYAARAISHGLRLP
ASLEALKPILVIPLLASLVTGLLMLYVVGKPVAGMLAALTGFLDGMGTSNAILLGLLLGGMMCVDLGGPVNK
AAYAFSVGLLSSHSYAPMAAVMAAGMVPPIGMGLATLLARRKFAESERQAGKAASVLGLCFISEGAIPFAAK
DPLRVIPASIAGGALTGALSMYFGCKLQAPHGGLFVMLVPNAINHALAYLLAIVAGSLLTGLLYAVLKRGAE
PGLALGTSSSREPAAGG*
AnnotationID:6378GeneID:6377
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 16:04:25
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:(4) Inner membrane protein
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:protein-N(pi)-phosphohistidine-sugar phosphotransferase
Functional Category Confidence Code:5
COGs:COG1299
Secondary Functional Category(ies):carbon compound metabolism
EC Number:2.7.1.69
Status:ACTIVE
Pathway:
Glycolysis / Gluconeogenesis;
Fructose and mannose metabolism;
Galactose metabolism;
Starch and sucrose metabolism
PATH: map00530  Aminosugars metabolism
Homology:
>gb|AAO54490.1| (AE016859) phosphotransferase system, fructose-specific IIBC
           component [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 584

 Score =  664 bits (1712), Expect = 0.0
 Identities = 342/430 (79%), Positives = 385/430 (89%), Gaps = 1/430 (0%)

>gb|AAC75228.1| (AE000306) PTS system, fructose-specific transport protein
           [Escherichia coli K12]
          Length = 563

 Score =  552 bits (1422), Expect = e-156
 Identities = 277/433 (63%), Positives = 352/433 (81%), Gaps = 4/433 (0%)

>gb|AAC22105.1| (U32727) PTS system, fructose-specific IIBC component (fruA)
           [Haemophilus influenzae Rd]
          Length = 556

 Score =  441 bits (1133), Expect = e-123
 Identities = 228/433 (52%), Positives = 309/433 (71%), Gaps = 8/433 (1%)
Structural Features:
COG1299, FruA, Phosphotransferase system, fructose-specific IIC component 
[Carbohydrate transport and metabolism]
CD-Length = 343 residues, 100.0% aligned
Score =  334 bits (859), Expect = 2e-92
Identities = 207/344 (60%), Positives = 256/344 (74%), Gaps = 7/344 (2%)
Genomic Context:






   
Comment:
Inactivated 2/01/06.  PA14_18270 (GeneID 6379) and PA14_18290 (GeneID 6377) replaced by PA14_18275 (GeneID 7380).


















    ReferenceID:12574




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29861




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31984




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6377_cdd.html


    		




 
URL:

















    ReferenceID:34146




  
Author/Investigator(s):
        Ogawa M, Fujitani S, Mao X, Inouye S, Komano T.





  
Title:
        FruA, a putative transcription factor essential for the development of Myxococcus xanthus.




  
PubMed:
        8951822


MedLine:
        




    
Source:
Mol Microbiol. 1996 Nov;22(4):757-67.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8951822

















    ReferenceID:34147




  
Author/Investigator(s):
        Burne RA, Penders JE.





  
Title:
        Characterization of the Streptococcus mutans GS-5 fruA gene encoding exo-beta-D-fructosidase.




  
PubMed:
        1398976


MedLine:
        




    
Source:
Infect Immun. 1992 Nov;60(11):4621-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1398976

















    ReferenceID:34148




  
Author/Investigator(s):
        Wu LF, Saier MH Jr.





  
Title:
        Nucleotide sequence of the fruA gene, encoding the fructose permease of the Rhodobacter capsulatus phosphotransferase system, and analyses of the deduced protein sequence.




  
PubMed:
        2254279


MedLine:
        




    
Source:
J Bacteriol. 1990 Dec;172(12):7167-78.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2254279

















    ReferenceID:34149




  
Author/Investigator(s):
        Prior TI, Kornberg HL.





  
Title:
        Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12.




  
PubMed:
        3076173


MedLine:
        




    
Source:
J Gen Microbiol. 1988 Oct;134 ( Pt 10):2757-68.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3076173









           
Homologs By Global Alignment 

           

       



     
Gene ID:6377
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7980
                           PA3560_tr
                           translation of PA3560
                           585
                           76.75
                           76.75
                           136
                           2206.0
     

     
     
199057
                           gb|AAG06948.1|AE004777_1
                           (AE004777) phosphotransferase system, fructose-specific IIBC component [Pseudomonas aeruginosa PAO1]
                           585
                           76.75
                           76.75
                           136
                           2206.0
     

     
     
199060
                           gb|AAL47556.1|
                           (AY065999) fructose-specific phosphotransferase component II [Azotobacter vinelandii]
                           458
                           73.36
                           81.65
                           26
                           1646.0
     

     
     
199059
                           gb|AAN66420.1|AE016269_7
                           (AE016777) phosphotransferase system, fructose-specific IIBC component [Pseudomonas putida KT2440]
                           586
                           58.87
                           66.55
                           143
                           1713.0
     

     
     
199058
                           gb|AAO54490.1|
                           (AE016859) phosphotransferase system, fructose-specific IIBC component [Pseudomonas syringae pv. tomato str. DC3000]
                           592
                           58.61
                           65.87
                           151
                           1711.0
     

     






      

          
GC ORFID: 49379How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 3385992Stop: 3384643

          
Length: 1350


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3560 translation of PA3560Homolog Bit Score 854.0

          
Other Homologs:  t_PA3560 translation of PA3560 (BLASTP 854), psyr_15may02_Scaffold15_revised_gene2496 related to: gi|15598756|ref|NP_252250.1| (NC_002516) phosphotransferase system, fructose-specific IIBC component [Pseudomonas aeruginosa] pir||H83199 phosphotransferase system, fructose-specific IIBC component PA3560 [imported] - Pseudomonas aeruginosa -TRUNCATED- (BLASTP 669)

          
GC ORF Sequence

           
ATGGCCGCCGAGGCGTTGCAGCTCGCGGCGGGGCAACTGGGCTTCGCGTTGCAGGTCGAGACCCAGGGCTCG 
GTCGGCGCGCGCAACCCGCTCGACCCGGCGGACATCGCCGCCGCCGACGTGGTCCTGCTGGCCGCCGACATC 
GACGTGGACACGGCCCGTTTCGCCGGCAAGAAGATCTACCGTTGCGGCACCGGCGTGGCCCTCAAGCAGGCC 
CGCGCGACGCTCGAGCGGGCGCTGGCGGAAGGGCAGGTCGAGAGCGCGGGCGCAGCCAGCGCGGTGGTCGCC 
AGGGACGAGAAGCGCGGGGTGTACAAGCACCTGCTGACCGGGGTGTCGTTCATGTTGCCGATGGTGGTCGCG 
GGCGGCCTGCTGATCGCCCTGTCGCTGGCCTTCGGCATCGATGCCTACAAGCAGCCGGGCAGCCTGGCGGCG 
GTGCTGAGGACGGTCGGCGACACGGCTTTCGTCCTGATGGTGCCGATGCTCGCCGGCTACATCGCCTATTCC 
ATCGCCGACCGCCCGGGCCTGGCGCCGGGCATGCTCGGCGGCCTGCTCGCCGGGACCCTCGGCGCCGGGTTC 
ATCGGCGGGATAGTCGCCGGCTTCATCGCCGGCTATGCGGCGCGGGCCATCAGCCACGGGCTGAGGCTGCCG 
GCGAGCCTGGAAGCGCTGAAGCCGATCCTGGTCATCCCGTTGCTGGCCAGCCTGGTCACCGGCCTGCTGATG 
CTCTACGTGGTCGGCAAGCCGGTGGCCGGGATGCTCGCGGCACTGACCGGGTTCCTCGACGGCATGGGTACC 
TCCAATGCCATCCTCCTCGGCCTGCTGCTCGGCGGGATGATGTGCGTCGACCTCGGCGGACCGGTCAACAAG 
GCCGCCTACGCCTTTTCCGTCGGCCTGCTCTCGTCGCACAGCTACGCGCCGATGGCGGCGGTGATGGCGGCG 
GGCATGGTGCCGCCGATCGGCATGGGCCTGGCGACCCTGCTGGCGCGGCGCAAGTTCGCCGAGAGCGAGCGG 
CAGGCCGGCAAGGCAGCCTCGGTGCTGGGGCTGTGCTTCATCTCCGAAGGCGCGATCCCGTTCGCCGCCAAG 
GACCCGTTGCGGGTGATCCCGGCGAGCATCGCCGGCGGCGCGCTGACCGGCGCCCTGTCGATGTATTTCGGC 
TGCAAGTTGCAGGCGCCTCACGGCGGGTTGTTCGTGATGCTGGTGCCCAACGCGATCAACCACGCCCTGGCG 
TACCTGCTGGCGATCGTCGCCGGCAGCCTGCTTACCGGGCTGCTCTACGCCGTGCTCAAGCGCGGCGCCGAG 
CCGGGACTGGCGTTGGGGACCTCGTCCTCGCGGGAGCCGGCGGCTGGCGGATAG