ViewGene
Locus Name:
GeneID:
Location:

GeneID:6367
TrivialName: PA14_18560
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18560
PAO1 Ortholog Locus:
Sequence Length:1569
Protein Length:522
Start: 1593855
Stop: 1592288
Strand: -
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
GeneID6367 (PA14_18560) replaced by GeneID7418 (PA14_18565). Homology: gb|AAG06929.1|AE004774_7 (AE004774) alginate biosynthesis protein Alg8 [Pseudomonas aeruginosa PAO1]
Identities = 457/458 (99%), Positives = 457/458 (99%)
Sequence:
ATGGAAACTTACAAACGTGGCCTCGCCGAAGCCACCGGCTGGCTGGTGTTCCTCAGCCTGCTGATGGTGCTC
GCGCTGGCAGTGCCGAAGACCGTGTTCGACGCCGACTCCAAGGATTTCATCCTGCTTATCGGCGCCGTCGGC
ATCTGGCGCTACTCCATGGGCGGCGTGCACTTCCTGCGCGGCATGCTGTTCCTCCACGTGGTCTACCCGTAC
TACCGCCGGCACGTGCGCCAGTTGGGCAGCGCGGCCGACCCGTCGCACGTGTTCCTGATGGTCACCAGTTTC
CGCATCGACGCCCTGACCACTGCCATGGTCTATCGCTCGGTGATCCGCGAAGCCATCGACAGCGGCTACCCG
ACCACCGTGGTCTGCTCCATCGTCGAGATGTCCGACGAGGTCCTGGTCCGTTCTCTGTGGGAGAAGATGAAC
CCGCCGGACCGGGTCAGCCTCGACTTCGTGCGGATTCCCGGCACCGGCAAGCGCGACGGCCTGGCCTATGGC
TTCCGCGCGATTTCCCGGCACCTGCCGGACGACGACGCGGTGGTCGCGGTGATCGACGGCGACACCGTGCTC
GACCACGGCGTGGTGAAGAAGACCGTGCCCTGGTTCAAGCTCTTCCCCAACGTCGGCGGCCTCACCACCAAT
GAATTCTGCGAAGTGCAGGGCGGCTACGTGATGAGCGAGTGGCACAAGCTGCGCTTCGCCCAGCGCCACATC
AACATGTGCTCGATGGCCCTGTCCAAGCGCGTGCTGACCATGACCGGACGGATGTCGGTGTTCCGCGCCCGG
GTGGTGACCAACCCCGAGTTCATCACCGACGTCGAGAACGACCACCTGGAGCACTGGCGCCTGGGTCGCTTC
AAGTTCCTCACCGGTGACGACAAGTCCAGCTGGTTCAGCCTGATGCGCCTGGGCTACGACACCTTCTACGTG
CCCGACGCGGCGATCAACACGGTCGAGCACCCGCCGGAGAAGAGCTTCATCAAGGCCAGCCGCAAGCTGATG
TACCGCTGGTACGGCAACAACCTGCGGCAGAACTCGCGCGCGCTCAAGCTCGGCGCGCGGCGCCTGGGCTGG
TTCACCATGCTGGTGCTGTTCGACCAGCGCGTCTCGATGTGGACCAGCCTGCTCGGCCTGGTGGTGGCGATC
CTCGCCAGCCTCAAGTACAGCATCGCCTTCCTGCTGGTGTACCTGCTCTGGATCGGCCTCACCCGCCTGGTG
CTGACCCTGCTCCTCTCGCTGTCCGGGCACCGCATCGGCCCGGCCTATCCGCTGATCCTCTATTACAACCAG
ATCGTCGGCGCGCTGGTGAAGATCTACGTGTTCTTCcGCCTCGACCGGCAGTCCTGGACCCGCCAGCCGACC
AaGCtgggaGCGCGgccTGGCCAGCTTCCAGCGCTGGTTCAACGCCTGGTCGTCGCGGGCCATGACCTTCTC
TGCCGCCAGCATCTTCGTCGCCGTGCTGCTGACCATCGTATGACCACCGAATCGACTACGGACAACGCCAGG
GCCGCGGCCCGCGCGCAAATCGACCCAGATTGGACCGAACAGGACCCGACACCATGA
Translation:
METYKRGLAEATGWLVFLSLLMVLALAVPKTVFDADSKDFILLIGAVGIWRYSMGGVHFLRGMLFLHVVYPY
YRRHVRQLGSAADPSHVFLMVTSFRIDALTTAMVYRSVIREAIDSGYPTTVVCSIVEMSDEVLVRSLWEKMN
PPDRVSLDFVRIPGTGKRDGLAYGFRAISRHLPDDDAVVAVIDGDTVLDHGVVKKTVPWFKLFPNVGGLTTN
EFCEVQGGYVMSEWHKLRFAQRHINMCSMALSKRVLTMTGRMSVFRARVVTNPEFITDVENDHLEHWRLGRF
KFLTGDDKSSWFSLMRLGYDTFYVPDAAINTVEHPPEKSFIKASRKLMYRWYGNNLRQNSRALKLGARRLGW
FTMLVLFDQRVSMWTSLLGLVVAILASLKYSIAFLLVYLLWIGLTRLVLTLLLSLSGHRIGPAYPLILYYNQ
IVGALVKIYVFFRLDRQSWTRQPTKLGARPGQLPALVQRLVVAGHDLLCRQHLRRRAADHRMTTESTTDNAR
AAARAQIDPDWTEQDPTP*
AnnotationID:6368GeneID:6367
AnnotatorUID: danlee
Modification Date/Time: 2006-03-11 16:04:17
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:2.4.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54767.1| (AE016860) alginate biosynthesis protein Alg8 [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 493

 Score =  786 bits (2031), Expect = 0.0
 Identities = 382/458 (83%), Positives = 419/458 (91%)

>emb|CAA70053.1| (Y08819) glucosyl transferase [Azotobacter vinelandii]
          Length = 492

 Score =  709 bits (1830), Expect = 0.0
 Identities = 351/458 (76%), Positives = 395/458 (86%), Gaps = 1/458 (0%)

>gb|AAC07558.1| (AE000751) alginate synthesis-related protein [Aquifex aeolicus
           VF5]
          Length = 882

 Score =  280 bits (717), Expect = 1e-74
 Identities = 164/448 (36%), Positives = 256/448 (57%), Gaps = 15/448 (3%)








 
Structural Features:






   
Genomic Context:






   
Comment:
GeneID6367 (PA14_18560) replaced by GeneID7418 (PA14_18565).


















    ReferenceID:12556




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29842




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32023




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6367_cdd.html


    		




 
URL:

















    ReferenceID:34127




  
Author/Investigator(s):
        Maharaj R, May TB, Wang SK, Chakrabarty AM.





  
Title:
        Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.




  
PubMed:
        8294014


MedLine:
        




    
Source:
Gene. 1993 Dec 22;136(1-2):267-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8294014









           
Homologs By Global Alignment 

           

       



     
Gene ID:6367
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7979
                           PA3541_tr
                           translation of PA3541
                           543
                           84.89
                           85.45
                           70
                           2364.0
     

     
     
199052
                           gb|AAG06929.1|AE004774_7
                           (AE004774) alginate biosynthesis protein Alg8 [Pseudomonas aeruginosa PAO1]
                           543
                           84.89
                           85.45
                           70
                           2364.0
     

     
     
199053
                           gb|AAC36875.2|
                           (L22611) alginate synthesis-related protein [Pseudomonas aeruginosa]
                           546
                           84.24
                           84.79
                           73
                           2359.0
     

     
     
199054
                           gb|AAO54767.1|
                           (AE016860) alginate biosynthesis protein Alg8 [Pseudomonas syringae pv. tomato str. DC3000]
                           541
                           71.16
                           78.37
                           67
                           2015.0
     

     
     
199055
                           gb|AAN66911.1|AE016319_7
                           (AE016778) alginate biosynthesis protein Alg8 [Pseudomonas putida KT2440]
                           544
                           68.19
                           77.20
                           70
                           1956.0
     

     






      

          
GC ORFID: 49369How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 3360079Stop: 3361647

          
Length: 1569


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3541 translation of PA3541Homolog Bit Score 921.0

          
Other Homologs:  t_PA3541 translation of PA3541 (BLASTP 921), psyr_15may02_Scaffold3_revised_gene4992 related to: gi|15598737|ref|NP_252231.1| (NC_002516) alginate biosynthesis protein Alg8 [Pseudomonas aeruginosa] pir||A83204 alginate biosynthesis protein Alg8 PA3541 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG06929.1|AE004774_7 (AE004774) algin-TRUNCATED- (BLASTP 783)

          
GC ORF Sequence

           
ATGGAAACTTACAAACGTGGCCTCGCCGAAGCCACCGGCTGGCTGGTGTTCCTCAGCCTGCTGATGGTGCTC 
GCGCTGGCAGTGCCGAAGACCGTGTTCGACGCCGACTCCAAGGATTTCATCCTGCTTATCGGCGCCGTCGGC 
ATCTGGCGCTACTCCATGGGCGGCGTGCACTTCCTGCGCGGCATGCTGTTCCTCCACGTGGTCTACCCGTAC 
TACCGCCGGCACGTGCGCCAGTTGGGCAGCGCGGCCGACCCGTCGCACGTGTTCCTGATGGTCACCAGTTTC 
CGCATCGACGCCCTGACCACTGCCATGGTCTATCGCTCGGTGATCCGCGAAGCCATCGACAGCGGCTACCCG 
ACCACCGTGGTCTGCTCCATCGTCGAGATGTCCGACGAGGTCCTGGTCCGTTCTCTGTGGGAGAAGATGAAC 
CCGCCGGACCGGGTCAGCCTCGACTTCGTGCGGATTCCCGGCACCGGCAAGCGCGACGGCCTGGCCTATGGC 
TTCCGCGCGATTTCCCGGCACCTGCCGGACGACGACGCGGTGGTCGCGGTGATCGACGGCGACACCGTGCTC 
GACCACGGCGTGGTGAAGAAGACCGTGCCCTGGTTCAAGCTCTTCCCCAACGTCGGCGGCCTCACCACCAAT 
GAATTCTGCGAAGTGCAGGGCGGCTACGTGATGAGCGAGTGGCACAAGCTGCGCTTCGCCCAGCGCCACATC 
AACATGTGCTCGATGGCCCTGTCCAAGCGCGTGCTGACCATGACCGGACGGATGTCGGTGTTCCGCGCCCGG 
GTGGTGACCAACCCCGAGTTCATCACCGACGTCGAGAACGACCACCTGGAGCACTGGCGCCTGGGTCGCTTC 
AAGTTCCTCACCGGTGACGACAAGTCCAGCTGGTTCAGCCTGATGCGCCTGGGCTACGACACCTTCTACGTG 
CCCGACGCGGCGATCAACACGGTCGAGCACCCGCCGGAGAAGAGCTTCATCAAGGCCAGCCGCAAGCTGATG 
TACCGCTGGTACGGCAACAACCTGCGGCAGAACTCGCGCGCGCTCAAGCTCGGCGCGCGGCGCCTGGGCTGG 
TTCACCATGCTGGTGCTGTTCGACCAGCGCGTCTCGATGTGGACCAGCCTGCTCGGCCTGGTGGTGGCGATC 
CTCGCCAGCCTCAAGTACAGCATCGCCTTCCTGCTGGTGTACCTGCTCTGGATCGGCCTCACCCGCCTGGTG 
CTGACCCTGCTCCTCTCGCTGTCCGGGCACCGCATCGGCCCGGCCTATCCGCTGATCCTCTATTACAACCAG 
ATCGTCGGCGCGCTGGTGAAGATCTACGTGTTCTTCcGCCTCGACCGGCAGTCCTGGACCCGCCAGCCGACC 
AaGCtgggaGCGCGgccTGGCCAGCTTCCAGCGCTGGTTCAACGCCTGGTCGTCGCGGGCCATGACCTTCTC 
TGCCGCCAGCATCTTCGTCGCCGTGCTGCTGACCATCGTATGACCACCGAATCGACTACGGACAACGCCAGG 
GCCGCGGCCCGCGCGCAAATCGACCCAGATTGGACCGAACAGGACCCGACACCATGA