ViewGene
Locus Name:
GeneID:
Location:

GeneID:6248
TrivialName: PA14_24450
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_24450
PAO1 Ortholog Locus:
Sequence Length:3528
Protein Length:1175
Start: 2130044
Stop: 2133570
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
Inactivated 2/03/06. PA14_24450 (GeneID 6248) and PA14_24460 (GeneID 6247) replaced by PA14_24455 (GeneID 7388). Homology: gb|AAG06456.1|AE004731_4 (AE004731) NAD-dependent glutamate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 944/944 (100%), Positives = 944/944 (100%)
Sequence:
ATGGCGTTCTTCACCGCGGCCAGCAAAGCCGATTTCCAGCATCAACTGCAGACGGCACTGGCGCAGCACCTC
GGCGACAAGGCGTTACCACAAGTCACGCTGTTCGCCGAACAGTTCTTCAGCCTGATCTCCCTCGATGAACTG
ACCCAGCGTCGGCTTTCCGACCTGGTCGGTTGCACCCTTTCCGCCTGGCGCCTGCTGGAGCGCTTCGACCGC
GACCAGCCCGAAGTCCGCGTATACAACCCCGACTACGAGAAGCACGGCTGGCAATCCACCCACACCGCGGTG
GAAGTGCTGCACCCGGACCTGCCGTTCCTGGTCGACTCGGTGCGCATGGAACTGAACCGTCGCGGCTACAGC
ATCCATACCCTGCAGACCAACGTCCTCAGCGTGCGCCGCTCGGCCAAGGGCGAGCTGAAGGAAATCCTGCCC
AAGGGTAGCCAAGGCAAGGACGTCAGCCAGGAATCGCTGATGTACCTGGAGATCGACCGTTGCGCCCACGCC
GGCGAACTGCGGGCGCTGGAGAAGGCCATCCTGGAAGTGCTCGGTGAAGTACGCGTGACCGTCGCCGACTTC
GAACCGATGAAGGCCAAGGCCCGCGAACTGCTGACCTGGCTTGGCAAGGCCAAGCTGAAGGTGCCGGCGGAG
GAACTGAAGGAAGTGCGCAGCTATCTCGAATGGCTGCTGGACAACCACTTCACCTTCCTCGGCTACGAGGAA
TTCAGCGTCGCCGACGAGGCCGACGGCGGGCGCATGGTCTACGACGAGAAATCCTTCCTCGGCCTGACCCGT
CTGCTGCGTGCCGGCCTGAGCAAGGATGACCTGCACATCGAGGACTATGCCGTCGCCTACCTGCGCGAACCG
GTCCTGCTGTCCTTCGCCAAGGCTGCCCATCCGAGCCGCGTGCACCGCCCGGCCTACCCGGACTACGTGTCG
ATCCGCGAGCTGGACGGCAAGGGCCGGGTGATCCGCGAGTGCCGCTTCATGGGCCTGTTCACCTCCTCGGTG
TACAACGAGAGCGTCAACGACATTCCCTTCATCCGCGGCAAGGTCGCCGAAGTGATGCGGCGCTCCGGTTTC
GACACCAAGGCCCACCTCGGCAAGGAACTGGCCCAGGTGCTGGAAGTACTGCCGCGCGACGACCTGTTCCAG
ACCCCGGTGGACGAGCTGTTCAGCACCGCCCTGGCGATCGTCCGCATCCAGGAGCGCAACAAGATCCGCGTG
TTCCTGCGCAAGGACCCCTACGGTCGCTTCTGCTACTGCCTGGCCTACGTGCCGCGCGACGTGTATTCCACC
GAGACGCGCCTGAAGATCCAGCAGGTCCTGATGGAGCGCCTGCAAGCCAGCGACTGCGAGTTCTGGACTTTC
TTCTCCGAGTCGGTGCTGGCCCGCGTGCAGTTCATCCTCCGCGTCGACCCGAAGTCGCGCATCGACATCGAC
CCGGCGCGCCTGGAGGAAGAAGTGATCCAGGCCTGCCGTTCCTGGCAGGACGACTACAGCAGCCTGGTGGTG
GAAAACCTCGGCGAGGCCAAGGGCACCAACGTCCTGGCCGACTTCCCGAAAGGCTTCCCGGCCGGCTATCGC
GAGCGCTTCGCCCCGCACTTCGCGGTGGTCGACCTGCAGCACCTGCTCAGCCTGTCCGAGCAGCGCCCGCTG
GTGATGAGCTTCTACCAGCCGCTGGCGCAGGGCGAGCAGCAACTGCACTGCAAGCTGTACCACGCCGACACC
CCGCTGGCCCTGTCCGACGTCCTGCCGATCCTGGAGAACCTCGGCCTGCGCGTGCTCGGCGAATTCCCCTAC
CGCCTGCGCCACCAGAACGGCCGCGAATACTGGATCCACGACTTCGCCTTCACCTACGCCGAAGGGCTGGAC
GTGGACATCCAGCAGCTCAACGAGATCCTCCAGGACGCCTTCGTCCACATCGTCAGCGGCGACGCCGAGAAC
GACGCCTTCAACCGCCTGGTGCTGACCGCCAACCTGCCGTGGCGCGACGTCGCCCTGCTGCGTGCCTACGCG
CGTTACCTGAAGCAGATCCGCCTGGGCTTCGACCTCGGCTACATCGCCAGTGCGCTGAATGCCCACACCGAC
ATCGCCCGCGAGCTGGTGCGGCTGTTCAAGACCCGCTTCTACCTGGCGCGCAAACTCACCGCGGAAGACCTG
GAAGACAAGCAACAGAAGCTGGAGCAGGCGATCCTCGGTGCCCTCGACGAAGTCCAGGTGCTCAACGAGGAC
CGCATCCTGCGGCGCTACCTCGACCTGATCAAGGCAACCCTGCGGACCAACTTCTACCAGCCCGACGGCAAC
GGCCAGAACAAGAGCTACTTCAGCTTCAAGTTCAACCCGAAGGCCATCCCGGAGCTGCCCAGGCCGGTGCCC
AAGTACGAGATCTTCGTCTACTCGCCGCGCGTCGAGGGCGTGCACCTGCGCGGCGGCAAGGTGGCTCGCGGC
GGCTTGCGCTGGTCGGACCGCGAGGAAGACTTCCGCACCGAGGTGCTCGGCCTGGTCAAGGCCCAGCAGGTG
AAGAACGCGGTGATCGTGCCGGTCGGCGCCAAGGGCGGCTTCGTCCCGCGCCGCCTGCCGCTTGGCGGTAGC
CGCGACGAGATCCAGGCCGAGGCGATCGCCTGCTACCGGATCTTCATTTCCGGCCTGCTCGACATCACCGAC
AACCTCAAGGAAGGCGAAGTGGTGCCGCCGGCCAACGTGGTCCGCCACGACGAGGACGATCCCTACCTGGTG
GTCGCCGCGGACAAGGGCACGGCGACCTTCTCCGACATCGCCAACGGCATCGCCGCCGAGTACGGTTTCTGG
CTCGGCGACGCCTTCGCCTCGGGGCGGCTCCGCCGGCTACGACCACAAGGGCATGGGGATTACCGCCAAGGG
CGCCTGGGTCAGCGTGCAACGGCACTTCCGCGAACGCGGCATCGACGTGCAGAAGGACAACATCAGCGTCAT
CGGCATCGGCGACATGGCCGGCGACGTGTTCGGCAACGGCCTGCTGATGTCGGACAAGCTGCAACTGGTGGC
CGCCTTCAACCACATGCATATCTTCATCGACCCGAACCCGGACGCCGCCAGCAGCTTCGTCGAGCGCCAGCG
CCTGTTCAACCTGCCGCGTTCGAGCTGGGCCGACTACGACGCCAAGCTGATCTCCGCCGGCGGCGGGATCTT
CCTGCGCAGCGCCAAGAGCATCGCCATCACCCCGGAAATGCAGGCGCGCTTCGACATCCAGGCCGACAGGCT
GGCGCCCACCGAGCTGATCCACGCGCTGCTCAAGGCGCCGGTCGACCTGCTCTGGAACGGCGGTATCGGCAC
CTACGTGAAGTCGAGCAAGGAAACCCACGCCGATGTCGGCGACAAGGCCAACGACGGTCTGCGCGTCGACGG
CCGCGAGCTGCGCGCGAAGGTGGTGGGCGAGGGCGGCAACCTGGGCATGACCCAACTGGCGCGGGTCGAGTT
CGGCCTGCACGGCGGTGCCAACAACACCGATTTCATCGACAACGTCGGTGGCGTGGACTGCTCCGACCATGA
Translation:
MAFFTAASKADFQHQLQTALAQHLGDKALPQVTLFAEQFFSLISLDELTQRRLSDLVGCTLSAWRLLERFDR
DQPEVRVYNPDYEKHGWQSTHTAVEVLHPDLPFLVDSVRMELNRRGYSIHTLQTNVLSVRRSAKGELKEILP
KGSQGKDVSQESLMYLEIDRCAHAGELRALEKAILEVLGEVRVTVADFEPMKAKARELLTWLGKAKLKVPAE
ELKEVRSYLEWLLDNHFTFLGYEEFSVADEADGGRMVYDEKSFLGLTRLLRAGLSKDDLHIEDYAVAYLREP
VLLSFAKAAHPSRVHRPAYPDYVSIRELDGKGRVIRECRFMGLFTSSVYNESVNDIPFIRGKVAEVMRRSGF
DTKAHLGKELAQVLEVLPRDDLFQTPVDELFSTALAIVRIQERNKIRVFLRKDPYGRFCYCLAYVPRDVYST
ETRLKIQQVLMERLQASDCEFWTFFSESVLARVQFILRVDPKSRIDIDPARLEEEVIQACRSWQDDYSSLVV
ENLGEAKGTNVLADFPKGFPAGYRERFAPHFAVVDLQHLLSLSEQRPLVMSFYQPLAQGEQQLHCKLYHADT
PLALSDVLPILENLGLRVLGEFPYRLRHQNGREYWIHDFAFTYAEGLDVDIQQLNEILQDAFVHIVSGDAEN
DAFNRLVLTANLPWRDVALLRAYARYLKQIRLGFDLGYIASALNAHTDIARELVRLFKTRFYLARKLTAEDL
EDKQQKLEQAILGALDEVQVLNEDRILRRYLDLIKATLRTNFYQPDGNGQNKSYFSFKFNPKAIPELPRPVP
KYEIFVYSPRVEGVHLRGGKVARGGLRWSDREEDFRTEVLGLVKAQQVKNAVIVPVGAKGGFVPRRLPLGGS
RDEIQAEAIACYRIFISGLLDITDNLKEGEVVPPANVVRHDEDDPYLVVAADKGTATFSDIANGIAAEYGFW
LGDAFASGRLRRLRPQGHGDYRQGRLGQRATALPRTRHRRAEGQHQRHRHRRHGRRRVRQRPADVGQAATGG
RLQPHAYLHRPEPGRRQQLRRAPAPVQPAAFELGRLRRQADLRRRRDLPAQRQEHRHHPGNAGALRHPGRQA
GAHRADPRAAQGAGRPALERRYRHLREVEQGNPRRCRRQGQRRSARRRPRAAREGGGRGRQPGHDPTGAGRV
RPARRCQQHRFHRQRRWRGLLRP*
AnnotationID:6249GeneID:6248
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 16:04:11
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam05088,COG2902
Secondary Functional Category(ies):putative enzyme
EC Number:1.4.1.4
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAC59865.1| (AP005078) putative NAD-glutamate dehydrogenase [Vibrio
           parahaemolyticus]
          Length = 1613

 Score =  807 bits (2084), Expect = 0.0
 Identities = 422/918 (45%), Positives = 593/918 (64%), Gaps = 15/918 (1%)

>gb|AAG40620.1|AF218569_1 (AF218569) NAD-glutamate dehydrogenase [Streptomyces clavuligerus]
          Length = 1651

 Score =  732 bits (1889), Expect = 0.0
 Identities = 420/946 (44%), Positives = 566/946 (59%), Gaps = 49/946 (5%)








 
Structural Features:
pfam05088, Bac_GDH, Bacterial NAD-glutamate dehydrogenase. 

COG2902, NAD-specific glutamate dehydrogenase [Amino acid transport and metabolism]






   
Genomic Context:






   
Comment:
Inactivated 2/03/06.  PA14_24450 (GeneID 6248) and PA14_24460 (GeneID 6247) 
replaced by PA14_24455 (GeneID 7388).


















    ReferenceID:12348




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29612




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32443




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6248_cdd.html


    		




 
URL:

















    ReferenceID:35947




  
Author/Investigator(s):
        Lu CD, Abdelal AT.





  
Title:
        The gdhB gene of Pseudomonas aeruginosa encodes an arginine-inducible NAD(+)-dependent glutamate dehydrogenase which is subject to allosteric regulation.




  
PubMed:
        11133942


MedLine:
        




    
Source:
J Bacteriol. 2001 Jan;183(2):490-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11133942









           
Homologs By Global Alignment 

           

       



     
Gene ID:6248
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7962
                           PA3068_tr
                           translation of PA3068
                           1669
                           59.85
                           61.17
                           543
                           4849.0
     

     
     
198762
                           gb|AAG06456.1|AE004731_4
                           (AE004731) NAD-dependent glutamate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           1669
                           59.85
                           61.17
                           543
                           4849.0
     

     
     
198763
                           gb|AAG53963.1|AF315586_1
                           (AF315586) NAD(+)-dependent glutamate dehydrogenase [Pseudomonas aeruginosa]
                           1669
                           59.85
                           61.17
                           543
                           4849.0
     

     
     
198765
                           gb|AAO57226.1|
                           (AE016869) conserved hypothetical protein [Pseudomonas syringae pv. tomato str. DC3000]
                           1679
                           49.55
                           55.27
                           565
                           4083.0
     

     
     
198764
                           gb|AAN67694.1|AE016400_2
                           (AE016781) conserved hypothetical protein [Pseudomonas putida KT2440]
                           1736
                           48.38
                           53.45
                           634
                           4101.0
     

     






      

          
GC ORFID: 49250How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 2823886Stop: 2820359

          
Length: 3528


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3068 translation of PA3068Homolog Bit Score 1879.0

          
Other Homologs:  t_PA3068 translation of PA3068 (BLASTP 1879), psyr_15may02_Scaffold1_revised_gene753 related to: gi|15598264|ref|NP_251758.1| (NC_002516) conserved hypothetical protein [Pseudomonas aeruginosa] pir||E83261 conserved hypothetical protein PA3068 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG06456.1|AE004731_4 (AE004731) conserved h-TRUNCATED- (BLASTP 1582)

          
GC ORF Sequence

           
ATGGCGTTCTTCACCGCGGCCAGCAAAGCCGATTTCCAGCATCAACTGCAGACGGCACTGGCGCAGCACCTC 
GGCGACAAGGCGTTACCACAAGTCACGCTGTTCGCCGAACAGTTCTTCAGCCTGATCTCCCTCGATGAACTG 
ACCCAGCGTCGGCTTTCCGACCTGGTCGGTTGCACCCTTTCCGCCTGGCGCCTGCTGGAGCGCTTCGACCGC 
GACCAGCCCGAAGTCCGCGTATACAACCCCGACTACGAGAAGCACGGCTGGCAATCCACCCACACCGCGGTG 
GAAGTGCTGCACCCGGACCTGCCGTTCCTGGTCGACTCGGTGCGCATGGAACTGAACCGTCGCGGCTACAGC 
ATCCATACCCTGCAGACCAACGTCCTCAGCGTGCGCCGCTCGGCCAAGGGCGAGCTGAAGGAAATCCTGCCC 
AAGGGTAGCCAAGGCAAGGACGTCAGCCAGGAATCGCTGATGTACCTGGAGATCGACCGTTGCGCCCACGCC 
GGCGAACTGCGGGCGCTGGAGAAGGCCATCCTGGAAGTGCTCGGTGAAGTACGCGTGACCGTCGCCGACTTC 
GAACCGATGAAGGCCAAGGCCCGCGAACTGCTGACCTGGCTTGGCAAGGCCAAGCTGAAGGTGCCGGCGGAG 
GAACTGAAGGAAGTGCGCAGCTATCTCGAATGGCTGCTGGACAACCACTTCACCTTCCTCGGCTACGAGGAA 
TTCAGCGTCGCCGACGAGGCCGACGGCGGGCGCATGGTCTACGACGAGAAATCCTTCCTCGGCCTGACCCGT 
CTGCTGCGTGCCGGCCTGAGCAAGGATGACCTGCACATCGAGGACTATGCCGTCGCCTACCTGCGCGAACCG 
GTCCTGCTGTCCTTCGCCAAGGCTGCCCATCCGAGCCGCGTGCACCGCCCGGCCTACCCGGACTACGTGTCG 
ATCCGCGAGCTGGACGGCAAGGGCCGGGTGATCCGCGAGTGCCGCTTCATGGGCCTGTTCACCTCCTCGGTG 
TACAACGAGAGCGTCAACGACATTCCCTTCATCCGCGGCAAGGTCGCCGAAGTGATGCGGCGCTCCGGTTTC 
GACACCAAGGCCCACCTCGGCAAGGAACTGGCCCAGGTGCTGGAAGTACTGCCGCGCGACGACCTGTTCCAG 
ACCCCGGTGGACGAGCTGTTCAGCACCGCCCTGGCGATCGTCCGCATCCAGGAGCGCAACAAGATCCGCGTG 
TTCCTGCGCAAGGACCCCTACGGTCGCTTCTGCTACTGCCTGGCCTACGTGCCGCGCGACGTGTATTCCACC 
GAGACGCGCCTGAAGATCCAGCAGGTCCTGATGGAGCGCCTGCAAGCCAGCGACTGCGAGTTCTGGACTTTC 
TTCTCCGAGTCGGTGCTGGCCCGCGTGCAGTTCATCCTCCGCGTCGACCCGAAGTCGCGCATCGACATCGAC 
CCGGCGCGCCTGGAGGAAGAAGTGATCCAGGCCTGCCGTTCCTGGCAGGACGACTACAGCAGCCTGGTGGTG 
GAAAACCTCGGCGAGGCCAAGGGCACCAACGTCCTGGCCGACTTCCCGAAAGGCTTCCCGGCCGGCTATCGC 
GAGCGCTTCGCCCCGCACTTCGCGGTGGTCGACCTGCAGCACCTGCTCAGCCTGTCCGAGCAGCGCCCGCTG 
GTGATGAGCTTCTACCAGCCGCTGGCGCAGGGCGAGCAGCAACTGCACTGCAAGCTGTACCACGCCGACACC 
CCGCTGGCCCTGTCCGACGTCCTGCCGATCCTGGAGAACCTCGGCCTGCGCGTGCTCGGCGAATTCCCCTAC 
CGCCTGCGCCACCAGAACGGCCGCGAATACTGGATCCACGACTTCGCCTTCACCTACGCCGAAGGGCTGGAC 
GTGGACATCCAGCAGCTCAACGAGATCCTCCAGGACGCCTTCGTCCACATCGTCAGCGGCGACGCCGAGAAC 
GACGCCTTCAACCGCCTGGTGCTGACCGCCAACCTGCCGTGGCGCGACGTCGCCCTGCTGCGTGCCTACGCG 
CGTTACCTGAAGCAGATCCGCCTGGGCTTCGACCTCGGCTACATCGCCAGTGCGCTGAATGCCCACACCGAC 
ATCGCCCGCGAGCTGGTGCGGCTGTTCAAGACCCGCTTCTACCTGGCGCGCAAACTCACCGCGGAAGACCTG 
GAAGACAAGCAACAGAAGCTGGAGCAGGCGATCCTCGGTGCCCTCGACGAAGTCCAGGTGCTCAACGAGGAC 
CGCATCCTGCGGCGCTACCTCGACCTGATCAAGGCAACCCTGCGGACCAACTTCTACCAGCCCGACGGCAAC 
GGCCAGAACAAGAGCTACTTCAGCTTCAAGTTCAACCCGAAGGCCATCCCGGAGCTGCCCAGGCCGGTGCCC 
AAGTACGAGATCTTCGTCTACTCGCCGCGCGTCGAGGGCGTGCACCTGCGCGGCGGCAAGGTGGCTCGCGGC 
GGCTTGCGCTGGTCGGACCGCGAGGAAGACTTCCGCACCGAGGTGCTCGGCCTGGTCAAGGCCCAGCAGGTG 
AAGAACGCGGTGATCGTGCCGGTCGGCGCCAAGGGCGGCTTCGTCCCGCGCCGCCTGCCGCTTGGCGGTAGC 
CGCGACGAGATCCAGGCCGAGGCGATCGCCTGCTACCGGATCTTCATTTCCGGCCTGCTCGACATCACCGAC 
AACCTCAAGGAAGGCGAAGTGGTGCCGCCGGCCAACGTGGTCCGCCACGACGAGGACGATCCCTACCTGGTG 
GTCGCCGCGGACAAGGGCACGGCGACCTTCTCCGACATCGCCAACGGCATCGCCGCCGAGTACGGTTTCTGG 
CTCGGCGACGCCTTCGCCTCGGGGCGGCTCCGCCGGCTACGACCACAAGGGCATGGGGATTACCGCCAAGGG 
CGCCTGGGTCAGCGTGCAACGGCACTTCCGCGAACGCGGCATCGACGTGCAGAAGGACAACATCAGCGTCAT 
CGGCATCGGCGACATGGCCGGCGACGTGTTCGGCAACGGCCTGCTGATGTCGGACAAGCTGCAACTGGTGGC 
CGCCTTCAACCACATGCATATCTTCATCGACCCGAACCCGGACGCCGCCAGCAGCTTCGTCGAGCGCCAGCG 
CCTGTTCAACCTGCCGCGTTCGAGCTGGGCCGACTACGACGCCAAGCTGATCTCCGCCGGCGGCGGGATCTT 
CCTGCGCAGCGCCAAGAGCATCGCCATCACCCCGGAAATGCAGGCGCGCTTCGACATCCAGGCCGACAGGCT 
GGCGCCCACCGAGCTGATCCACGCGCTGCTCAAGGCGCCGGTCGACCTGCTCTGGAACGGCGGTATCGGCAC 
CTACGTGAAGTCGAGCAAGGAAACCCACGCCGATGTCGGCGACAAGGCCAACGACGGTCTGCGCGTCGACGG 
CCGCGAGCTGCGCGCGAAGGTGGTGGGCGAGGGCGGCAACCTGGGCATGACCCAACTGGCGCGGGTCGAGTT 
CGGCCTGCACGGCGGTGCCAACAACACCGATTTCATCGACAACGTCGGTGGCGTGGACTGCTCCGACCATGA