ViewGene
Locus Name:
GeneID:
Location:

GeneID:6229
TrivialName: PA14_25310
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25310
PAO1 Ortholog Locus:
Sequence Length:216
Protein Length:71
Start: 2214182
Stop: 2214397
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Inactivated 1/02/06. PA14_25300 and PA14_25310 replaced by PA14_25305 (GeneID 7375). Homology: gb|AAG06386.1|AE004724_15 (AE004724) Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq2 [Pseudomonas aeruginosa PAO1]
Identities = 71/71 (100%), Positives = 71/71 (100%)
Sequence:
ATGGCCACCGACCCGGTCTCGGCGTCCATGACCGATACCGGCAAGTGGCTGTTCGGTGCCCTGATCGGAGTG
ATGGTCATGCTGATCCGGGTGGTCAACCCGGCGTTCCCGGAAGGCATGATGCTGGCGATCCTGTTCGCCAAC
CTGTTCGCACCGCTGATCGACCATTTCGTCGTTCAGGCCAACATCAAGCGGAGGCTGGCGCGCAATGGCTAA
Translation:
MATDPVSASMTDTGKWLFGALIGVMVMLIRVVNPAFPEGMMLAILFANLFAPLIDHFVVQANIKRRLARNG*
AnnotationID:6230GeneID:6229
AnnotatorUID: danlee
Modification Date/Time: 2006-02-01 13:01:07
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:NADH:ubiquinone oxidoreductase, subunit B
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:1.6.5.-
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAF95438.1| (AE004300) NADH:ubiquinone oxidoreductase, Na translocating,
           hydrophobic membrane protein NqrB [Vibrio cholerae]
          Length = 427

 Score =  117 bits (292), Expect = 2e-26
 Identities = 57/71 (80%), Positives = 63/71 (88%)

>emb|CAB84034.1| (AL162754) putative Na(+)-translocating NADH-ubiquinone reductase
           subunit B [Neisseria meningitidis Z2491]
          Length = 410

 Score =  115 bits (289), Expect = 4e-26
 Identities = 56/70 (80%), Positives = 63/70 (90%)

>gb|AAC21837.1| (U32702) NADH:ubiquinone oxidoreductase, subunit B (nqrB)
           [Haemophilus influenzae Rd]
          Length = 411

 Score =  115 bits (288), Expect = 6e-26
 Identities = 56/69 (81%), Positives = 62/69 (89%)






 
Structural Features:






   
Genomic Context:






   
Comment:
Inactivated 1/02/06. PA14_25300 and PA14_25310 replaced by PA14_25305 (GeneID 7375)


















    ReferenceID:12310




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29576




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36057




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6229_cdd.html


    		




 
URL:

















    ReferenceID:36458




  
Author/Investigator(s):
        Hayashi M, Hirai K, Unemoto T.





  
Title:
        Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus.




  
PubMed:
        7729558


MedLine:
        




    
Source:
FEBS Lett. 1995 Apr 17;363(1-2):75-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95246889&dopt=Abstract

















    ReferenceID:36465




  
Author/Investigator(s):
        Beattie P, Tan K, Bourne RM, Leach D, Rich PR, Ward FB.





  
Title:
        Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus.




  
PubMed:
        7805867


MedLine:
        




    
Source:
FEBS Lett. 1994 Dec 19;356(2-3):333-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95104445&dopt=Abstract

















    ReferenceID:36470




  
Author/Investigator(s):
        Hayashi M, Hirai K, Unemoto T.





  
Title:
        Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli.




  
PubMed:
        7805866


MedLine:
        




    
Source:
FEBS Lett. 1994 Dec 19;356(2-3):330-2.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95104444&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:6229
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7957
                           PA2998_tr
                           translation of PA2998
                           403
                           17.61
                           17.61
                           332
                           358.0
     

     
     
198641
                           gb|AAG06386.1|AE004724_15
                           (AE004724) Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq2 [Pseudomonas aeruginosa PAO1]
                           403
                           17.61
                           17.61
                           332
                           358.0
     

     
     
198645
                           gb|AAF40996.1|
                           (AE002412) Na(+)-translocating NADH-quinone reductase, subunit B [Neisseria meningitidis MC58]
                           410
                           14.14
                           15.60
                           339
                           290.0
     

     
     
198644
                           emb|CAB84034.1|
                           (AL162754) putative Na(+)-translocating NADH-ubiquinone reductase subunit B [Neisseria meningitidis Z2491]
                           410
                           14.14
                           15.60
                           339
                           290.0
     

     
     
198647
                           gb|AAC21837.1|
                           (U32702) NADH:ubiquinone oxidoreductase, subunit B (nqrB) [Haemophilus influenzae Rd] [Haemophilus influenzae Rd KW20]
                           411
                           14.11
                           15.57
                           340
                           290.0
     

     






      

          
GC ORFID: 49231How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 2739743Stop: 2739528

          
Length: 216


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2998 translation of PA2998Homolog Bit Score 142.0

          
Other Homologs:  t_PA2998 translation of PA2998 (BLASTP 142)

          
GC ORF Sequence

           
ATGGCCACCGACCCGGTCTCGGCGTCCATGACCGATACCGGCAAGTGGCTGTTCGGTGCCCTGATCGGAGTG 
ATGGTCATGCTGATCCGGGTGGTCAACCCGGCGTTCCCGGAAGGCATGATGCTGGCGATCCTGTTCGCCAAC 
CTGTTCGCACCGCTGATCGACCATTTCGTCGTTCAGGCCAACATCAAGCGGAGGCTGGCGCGCAATGGCTAA