ViewGene
Locus Name:
GeneID:
Location:

GeneID:6042
TrivialName: PA14_32010
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32010
PAO1 Ortholog Locus:
Sequence Length:1014
Protein Length:337
Start: 2786857
Stop: 2787869
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
GeneID6042 (PA14_32010) replaced by GID7415 (PA14_32015). N-terminus of PA2520, czcA. Homology: gb|AAG05908.1|AE004679_2 (AE004679) RND divalent metal cation efflux transporter CzcA [Pseudomonas aeruginosa PAO1]
Identities = 317/337 (94%), Positives = 318/337 (94%)
Sequence:
ATGTTCGAACGCATCATCCAATTCGCCATCGAGCAACGCTGGCTGGTCCTGCTGGCGGTGCTGGGGATGGCC
GGGGTCGGCATTGGCAGCTACCAGAAGCTGTCCATCGACGCGGTCCCGGACATCACCAACGTCCAGGTGCAG
ATCAATACCGCCGCCCCCGGCTACTCGCCGCTGGAAGTGGAGCAGCGGATCACCTACCCGGTGGAGACCGTC
ATGGCCGGCCTGCCGGGTTTGCAGGAAACCCGTTCGCTGTCGCGGCCGGGGATTTCCCAGGTGACGGTGATC
TTCGAGGAAGGCACCGACATCTACTTCGCCCGCCAGCAGGTCAACGAACGCCTGAGCACGGCGCGCGAGCAA
CTGCCGGAAGACATCTCGCCGACCCTCGGGCCGATCTCCACCGGTCTCGGCGAGATCTACCTGTGGACGGTA
GAGGCCGAGGAGGGTGCGACCAAGGAGGACGGCAGCGCCTACACGCCCACCGACCTGCGCACCATCCAGGAC
TGGATCATCCGTCCGCAGTTGCGCAACGTGAAGGGCGTGGCCGAGATCAACACCATCGGCGGCTACGCTAAG
CAATTCCTCATCGCCCCGGATCCGAAGAAGCTCGCGGCCTACAAGCTGACCCTCGGCGACCTGCAGAACGCC
GTGCTGCGCAACAACGAGAACGTCGGCGCCGGCTACATCGAGCGGCGCGGCGAGCAGTTGCTGATCCGCGCG
CCGGGCCAGGTCAAGGACATGGACGATATCCGCGGGATCATCGTTTCCAACGTCGACGGCGTGCCGATCCGT
ATCCGCGACGTCGCCGAGGTCGGCCTGGGCAAGGAGCTGCGCACCGGCGCGGCCACCGAGAACGGCCGCGAA
GTGGTGCTCGGCACGGTGTTCATGCTGATCGGCGAGAACAGCcgggaaagtggcccacgcggtctgccagcg
cctggaggagatcaACCGGAccctgcccagggggtcactgcgatcaccgtctacgaccgcaccaccctgcat
gactag
Translation:
MFERIIQFAIEQRWLVLLAVLGMAGVGIGSYQKLSIDAVPDITNVQVQINTAAPGYSPLEVEQRITYPVETV
MAGLPGLQETRSLSRPGISQVTVIFEEGTDIYFARQQVNERLSTAREQLPEDISPTLGPISTGLGEIYLWTV
EAEEGATKEDGSAYTPTDLRTIQDWIIRPQLRNVKGVAEINTIGGYAKQFLIAPDPKKLAAYKLTLGDLQNA
VLRNNENVGAGYIERRGEQLLIRAPGQVKDMDDIRGIIVSNVDGVPIRIRDVAEVGLGKELRTGAATENGRE
VVLGTVFMLIGENSRESGPRGLPAPGGDQPDPAQGVTAITVYDRTTLHD*
AnnotationID:6043GeneID:6042
AnnotatorUID: danlee
Modification Date/Time: 2006-03-10 18:06:05
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAK52285.1| (AY026914) membrane-bound cation-proton-antiporter CzrA
           [Pseudomonas putida]
          Length = 1051

 Score =  606 bits (1563), Expect = e-173
 Identities = 317/337 (94%), Positives = 318/337 (94%)

>gb|AAN65677.1|AE016194_7 (AE016774) cobalt/zinc/cadmium efflux RND transporter, permease
           protein, CzcA family [Pseudomonas putida KT2440]
          Length = 1053

 Score =  490 bits (1262), Expect = e-138
 Identities = 248/335 (74%), Positives = 282/335 (84%)

>emb|CAA67084.1| (X98451) membrane-bound cation-proton-antiporter [Ralstonia
           metallidurans]
          Length = 1063

 Score =  476 bits (1224), Expect = e-133
 Identities = 237/337 (70%), Positives = 275/337 (81%)







 
Structural Features:






   
Genomic Context:






   
Comment:
GeneID6042 (PA14_32010) replaced by GID7415 (PA14_32015).  N-terminus of PA2520, czcA.



















    ReferenceID:11975




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29205




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33079




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6042_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6042
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7946
                           PA2520_tr
                           translation of PA2520
                           1061
                           30.16
                           30.25
                           734
                           1575.0
     

     
     
198118
                           gb|AAG05908.1|AE004679_2
                           (AE004679) RND divalent metal cation efflux transporter CzcA [Pseudomonas aeruginosa PAO1]
                           1061
                           30.16
                           30.25
                           734
                           1575.0
     

     
     
198119
                           gb|AAK52285.1|
                           (AY026914) membrane-bound cation-proton-antiporter CzrA [Pseudomonas putida]
                           1061
                           30.16
                           30.25
                           734
                           1575.0
     

     
     
198120
                           emb|CAB56471.1|
                           (Y14018) czrA protein [Pseudomonas aeruginosa]
                           1061
                           29.97
                           30.06
                           734
                           1562.0
     

     
     
198121
                           gb|AAN65677.1|AE016194_7
                           (AE016774) cobalt/zinc/cadmium efflux RND transporter, permease protein, CzcA family [Pseudomonas putida KT2440]
                           1063
                           23.61
                           26.90
                           736
                           1267.0
     

     






      

          
GC ORFID: 49044How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 2167069Stop: 2166056

          
Length: 1014


          
Start Codon: ATGTruncated Start:

          
Stop Codon: tagTruncated Stop: 

          
Homolog: t_PA2520 translation of PA2520Homolog Bit Score 606.0

          
Other Homologs:  t_PA2520 translation of PA2520 (BLASTP 606), psyr_15may02_Scaffold6_revised_gene6877 related to: gi|12484566|gb|AAG09629.1| (AY007258) CztA [Pseudomonas fluorescens] (BLASTP 485)

          
GC ORF Sequence

           
ATGTTCGAACGCATCATCCAATTCGCCATCGAGCAACGCTGGCTGGTCCTGCTGGCGGTGCTGGGGATGGCC 
GGGGTCGGCATTGGCAGCTACCAGAAGCTGTCCATCGACGCGGTCCCGGACATCACCAACGTCCAGGTGCAG 
ATCAATACCGCCGCCCCCGGCTACTCGCCGCTGGAAGTGGAGCAGCGGATCACCTACCCGGTGGAGACCGTC 
ATGGCCGGCCTGCCGGGTTTGCAGGAAACCCGTTCGCTGTCGCGGCCGGGGATTTCCCAGGTGACGGTGATC 
TTCGAGGAAGGCACCGACATCTACTTCGCCCGCCAGCAGGTCAACGAACGCCTGAGCACGGCGCGCGAGCAA 
CTGCCGGAAGACATCTCGCCGACCCTCGGGCCGATCTCCACCGGTCTCGGCGAGATCTACCTGTGGACGGTA 
GAGGCCGAGGAGGGTGCGACCAAGGAGGACGGCAGCGCCTACACGCCCACCGACCTGCGCACCATCCAGGAC 
TGGATCATCCGTCCGCAGTTGCGCAACGTGAAGGGCGTGGCCGAGATCAACACCATCGGCGGCTACGCTAAG 
CAATTCCTCATCGCCCCGGATCCGAAGAAGCTCGCGGCCTACAAGCTGACCCTCGGCGACCTGCAGAACGCC 
GTGCTGCGCAACAACGAGAACGTCGGCGCCGGCTACATCGAGCGGCGCGGCGAGCAGTTGCTGATCCGCGCG 
CCGGGCCAGGTCAAGGACATGGACGATATCCGCGGGATCATCGTTTCCAACGTCGACGGCGTGCCGATCCGT 
ATCCGCGACGTCGCCGAGGTCGGCCTGGGCAAGGAGCTGCGCACCGGCGCGGCCACCGAGAACGGCCGCGAA 
GTGGTGCTCGGCACGGTGTTCATGCTGATCGGCGAGAACAGCcgggaaagtggcccacgcggtctgccagcg 
cctggaggagatcaACCGGAccctgcccagggggtcactgcgatcaccgtctacgaccgcaccaccctgcat 
gactag