ViewGene
Locus Name:
GeneID:
Location:

GeneID:6040
TrivialName: PA14_32020
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32020
PAO1 Ortholog Locus:
Sequence Length:1128
Protein Length:375
Start: 2787916
Stop: 2789041
Strand: +
Type:
ChromosomeID:2
Status: INACTIVE
Frame Discrepancy: TRUE
Missense Discrepancy:TRUE
Comments:
GeneID6040 (PA14_32020) and GeneID6038 (PA14_32040) replaced by GID7416 (PA14_32025). C-terminus of PA2520, czcA. Homology: gb|AAG05908.1|AE004679_2 (AE004679) RND divalent metal cation efflux transporter CzcA [Pseudomonas aeruginosa PAO1]
Identities = 358/362 (98%), Positives = 361/362 (99%)
Sequence:
GTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTG
TCGATGCTGTTCACCTTCACCGGGATGGTCGGCAACAGGGTCAGCGCCAACCTGATGAGCCTCGGCGCGCTG
GACTTCGGCATCATCGTCGACGGCGCCGTGGTGATCGTCGAGAACGCCATCCGCCGCCTGGCCCATGCCCAG
GCCCATCACGGCCGCCAGCTGACCCGCGCGGAACGTTTCCACGAGGTCTTCGCCGCGTCCAGGGAGGCGCGC
CGGGCGCTGATCTTCGGCCAGATCATCATCATGGTGGTGTACCTGCCGATCTTCGCCCTCACCGGGGTCGAG
GGGAAGATGTTCCACCCGATGGCGTTCACCGTGGTCACCGCGCTGCTTGGCGCGATGATCCTCTCGGTGACC
TTCGTCCCGGCGGCGATCGCGCTGTTCATCACCGGCAAGGTCAAGGAGGAGGAGAACTTCGTCATGCGCCGC
GCGCGTCTGGCCTACGAACCGGCGCTGCGCTGGGTGCTCGGACACCGCGCGCTGGTGGTCGGCGGTGCCCTC
GGCGCGATCCTGCTCACCGCGCTGGTGGCCTCGCGGATGGGCAGCGAGTTCATTCCCAGCCTCAGCGAGGGC
GACTTCGCCATGCAGGGCCTGCGGGTGCCGGGCACCAGCCTGACCCAGTCGGTCGAGATGCAGCAGACCCTG
GAGAAGAAGCTGATGGGCAAGTTCCCGGAGATCGAGCGGGTCTTCGCCCGCACCGGGACCGCCGAGATTGCC
TCCGACCTGATGCCGCCGAACGCCTCCGACAGCTACGTGATGCTCAAGCCGCAGAGCCAGTGGCCGGACCCG
AAGAAGTCGCGGGAGGCGCTGCTGGAAGAATTGCAGGCCGCGGCCCTTGAGGTGCCGGGCAGCGTCTACGAG
TTCTCCCAGCCGATCCAGTTgcgctTCAAcgaACTGATCTCcGGGGTGCGCAGCGACGTCGCGGTGAAGgTC
TtcggCGACGaCATGCAGGTGCTCaaCGACACTGCGGAGAAGATCTcCAAGGTGCTGCAAGGCATCGACGGC
GcCtcccgagGTGAAGGTCGAGCAGaccCAcCGGcCTGCCGGTGCTGA
Translation:
MIAVLFLFLGNIRAALITATIIPLSMLFTFTGMVGNRVSANLMSLGALDFGIIVDGAVVIVENAIRRLAHAQ
AHHGRQLTRAERFHEVFAASREARRALIFGQIIIMVVYLPIFALTGVEGKMFHPMAFTVVTALLGAMILSVT
FVPAAIALFITGKVKEEENFVMRRARLAYEPALRWVLGHRALVVGGALGAILLTALVASRMGSEFIPSLSEG
DFAMQGLRVPGTSLTQSVEMQQTLEKKLMGKFPEIERVFARTGTAEIASDLMPPNASDSYVMLKPQSQWPDP
KKSREALLEELQAAALEVPGSVYEFSQPIQLRFNELISGVRSDVAVKVFGDDMQVLNDTAEKISKVLQGIDG
ASRGEGRADPPACRC*
AnnotationID:6041GeneID:6040
AnnotatorUID: danlee
Modification Date/Time: 2006-03-10 18:06:34
Gene Name:
Confidence Code:4
GeneProduct:
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:()
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:INACTIVE
Pathway:






 
Homology:
>gb|AAK52285.1| (AY026914) membrane-bound cation-proton-antiporter CzrA
           [Pseudomonas putida]
          Length = 1051

 Score =  686 bits (1769), Expect = 0.0
 Identities = 358/362 (98%), Positives = 361/362 (99%)

>gb|AAN65677.1|AE016194_7 (AE016774) cobalt/zinc/cadmium efflux RND transporter, permease
           protein, CzcA family [Pseudomonas putida KT2440]
          Length = 1053

 Score =  560 bits (1443), Expect = e-159
 Identities = 284/362 (78%), Positives = 319/362 (88%)

>emb|CAA67084.1| (X98451) membrane-bound cation-proton-antiporter [Ralstonia
           metallidurans]
          Length = 1063

 Score =  518 bits (1335), Expect = e-146
 Identities = 264/361 (73%), Positives = 309/361 (85%)








 
Structural Features:






   
Genomic Context:






   
Comment:
GeneID6040 (PA14_32020) and GeneID6038 (PA14_32040) replaced by GID7416 (PA14_32025).
C-terminus of PA2520, czcA.


















    ReferenceID:15889




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29201




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33078




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6040_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6040
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7945
                           PA2520_tr
                           translation of PA2520
                           1051
                           34.34
                           34.63
                           676
                           1762.0
     

     
     
198094
                           gb|AAG05908.1|AE004679_2
                           (AE004679) RND divalent metal cation efflux transporter CzcA [Pseudomonas aeruginosa PAO1]
                           1051
                           34.34
                           34.63
                           676
                           1762.0
     

     
     
198095
                           gb|AAK52285.1|
                           (AY026914) membrane-bound cation-proton-antiporter CzrA [Pseudomonas putida]
                           1051
                           34.34
                           34.63
                           676
                           1762.0
     

     
     
198096
                           emb|CAB56471.1|
                           (Y14018) czrA protein [Pseudomonas aeruginosa]
                           1051
                           34.25
                           34.44
                           676
                           1751.0
     

     
     
198098
                           gb|AAO53919.1|
                           (AE016857) cation efflux family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           1053
                           27.44
                           30.10
                           678
                           1422.0
     

     






      

          
GC ORFID: 49042How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 2166010Stop: 2164883

          
Length: 1128


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2520 translation of PA2520Homolog Bit Score 687.0

          
Other Homologs:  t_PA2520 translation of PA2520 (BLASTP 687), psyr_15may02_Scaffold6_revised_gene6877 related to: gi|12484566|gb|AAG09629.1| (AY007258) CztA [Pseudomonas fluorescens] (BLASTP 561)

          
GC ORF Sequence

           
GTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTG 
TCGATGCTGTTCACCTTCACCGGGATGGTCGGCAACAGGGTCAGCGCCAACCTGATGAGCCTCGGCGCGCTG 
GACTTCGGCATCATCGTCGACGGCGCCGTGGTGATCGTCGAGAACGCCATCCGCCGCCTGGCCCATGCCCAG 
GCCCATCACGGCCGCCAGCTGACCCGCGCGGAACGTTTCCACGAGGTCTTCGCCGCGTCCAGGGAGGCGCGC 
CGGGCGCTGATCTTCGGCCAGATCATCATCATGGTGGTGTACCTGCCGATCTTCGCCCTCACCGGGGTCGAG 
GGGAAGATGTTCCACCCGATGGCGTTCACCGTGGTCACCGCGCTGCTTGGCGCGATGATCCTCTCGGTGACC 
TTCGTCCCGGCGGCGATCGCGCTGTTCATCACCGGCAAGGTCAAGGAGGAGGAGAACTTCGTCATGCGCCGC 
GCGCGTCTGGCCTACGAACCGGCGCTGCGCTGGGTGCTCGGACACCGCGCGCTGGTGGTCGGCGGTGCCCTC 
GGCGCGATCCTGCTCACCGCGCTGGTGGCCTCGCGGATGGGCAGCGAGTTCATTCCCAGCCTCAGCGAGGGC 
GACTTCGCCATGCAGGGCCTGCGGGTGCCGGGCACCAGCCTGACCCAGTCGGTCGAGATGCAGCAGACCCTG 
GAGAAGAAGCTGATGGGCAAGTTCCCGGAGATCGAGCGGGTCTTCGCCCGCACCGGGACCGCCGAGATTGCC 
TCCGACCTGATGCCGCCGAACGCCTCCGACAGCTACGTGATGCTCAAGCCGCAGAGCCAGTGGCCGGACCCG 
AAGAAGTCGCGGGAGGCGCTGCTGGAAGAATTGCAGGCCGCGGCCCTTGAGGTGCCGGGCAGCGTCTACGAG 
TTCTCCCAGCCGATCCAGTTgcgctTCAAcgaACTGATCTCcGGGGTGCGCAGCGACGTCGCGGTGAAGgTC 
TtcggCGACGaCATGCAGGTGCTCaaCGACACTGCGGAGAAGATCTcCAAGGTGCTGCAAGGCATCGACGGC 
GcCtcccgagGTGAAGGTCGAGCAGaccCAcCGGcCTGCCGGTGCTGA