ViewGene
Locus Name:
GeneID:
Location:

GeneID:5944
TrivialName: PA14_35790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35790
PAO1 Ortholog Locus:
Sequence Length:1410
Protein Length:469
Start: 3184587
Stop: 3183178
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: emb|CAD85409.1| (BX321861) putative homospermidine synthase protein [Nitrosomonas europaea ATCC 19718]
Identities = 216/463 (46%), Positives = 298/463 (64%), Gaps = 5/463 (1%)
Sequence:
ATGGATTTTTCAATTAATCCCCCCCAGCGAATTGTCTTTGTGGGACTAGGAACGATTGCCCAGTCCTTCCTT
CCTCTCCTTTCTAAGGTTCACGACTTATCTACTTTAGAAATCTACGCGATCGATCCAAAAACACCACCGCTT
ATTGAGTACTTTGCTAACAGTTTTGGCTTAAAATTTATCAACTCTGCAATTGATCAAATCAACTATCGCGAC
ATTCTTGTGCCGATTCTTGGTGAAGGGACGGTTCTAATAAATCTATCAACCGATGTCTCAAGTCTTGCCCTG
ATAGAATTATGCCGCTCCGCAGGGGCTCTTTATTTAGACACATGCATTGAGCCGTGGAAGGGCGGATATGAT
GACCCAACCATACCACTGCATAAGCGAACTAACTATCACTTAAGAGAACAAATGCTGTCTCTGAAAAAGAGG
CTAGGCTCAGGGGTGACGGCTCTCGTGGCGCATGGCGCGAATCCTGGGTTGGTATCCCACTTCGTTAAACGT
GCTCTTTTAGATTTAGCGGAAGAGATATTGGGTGATTGTAAAAAGCCCTCTAATAAAGAGCAGTGGGCTATC
CTAAGCCAAAGATTGGGTGTCAAAGTAATCCATGTCGCAGAGTACGACTCGCAAATTTCTCAGAAATCTAGG
GAGAGGGGTGAGTTCGTAAATACTTGGTCAGTGCATGGGTTCATAAGTGAGTCGCAACAACCGGCAGAGTTG
GGCTGGGGAAGTCACGAGCGAAGTCTACCCACGGATGCGTCAATGCATACAGATGGATGCGGAGCAGCAATC
TATATTGAGAAGCCAGGGGCCTCTGTTAGAGTTAAGACATGGACGCCATTTAACGGCCCTAGCTTAGGTTAT
TTGGTAACTCATCACGAGGCAATTTCAATTGCTGACTTTTTGACGCTCAGAACTGCCGATGAGACATATAGA
CCAACTGTCCATTATGCCTATCGCCCAAGCGATGAAGCAATTCTCTCTGTACATGAATGGTTCGGCAATGAT
TGCATGACTCCAGAAAAGACAAAAGTACTTCGTCCTGGTGATATCTTGTCGGGTTCTGATTACCTCGGTGTT
CTTCTTATGGGGCACGAAAAATCTAGTTATTGGTATGGATCTATCTTAAGCATCGAAAAGGCCAAGGAACTT
GCCACTCTCAACACTGCAACAACGTTACAGGTAGCAGCCGGAGTTCTTTCCGGTTACTTATGGATCCTGAGC
CATCCGTCGGCAGGAATAATTGAAGCCGAAGACATGGATCATGAAGTGGCACTATCTTATATTTCACAGTAT
CTAGGCGAACTAAAAGGGGTTTATTCGGACTGGAATCCTACCAAAAACAACCCAGGCACCTTTTCGGCCATA
GATAGCGACTCTCCCTGGCTGTTCTCAAATTTTGTCCTCTGA
Translation:
MDFSINPPQRIVFVGLGTIAQSFLPLLSKVHDLSTLEIYAIDPKTPPLIEYFANSFGLKFINSAIDQINYRD
ILVPILGEGTVLINLSTDVSSLALIELCRSAGALYLDTCIEPWKGGYDDPTIPLHKRTNYHLREQMLSLKKR
LGSGVTALVAHGANPGLVSHFVKRALLDLAEEILGDCKKPSNKEQWAILSQRLGVKVIHVAEYDSQISQKSR
ERGEFVNTWSVHGFISESQQPAELGWGSHERSLPTDASMHTDGCGAAIYIEKPGASVRVKTWTPFNGPSLGY
LVTHHEAISIADFLTLRTADETYRPTVHYAYRPSDEAILSVHEWFGNDCMTPEKTKVLRPGDILSGSDYLGV
LLMGHEKSSYWYGSILSIEKAKELATLNTATTLQVAAGVLSGYLWILSHPSAGIIEAEDMDHEVALSYISQY
LGELKGVYSDWNPTKNNPGTFSAIDSDSPWLFSNFVL*
AnnotationID:5945GeneID:5944
AnnotatorUID: danlee
Modification Date/Time: 2005-10-10 16:04:37
Gene Name:
Confidence Code:3
GeneProduct:Putative homospermidine synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06408
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|68545259|ref|ZP_00584810.1|  Homospermidine synthase [Shewanella amazonensis SB2B]
     Length=471
     Score =  454 bits (1167),  Expect = 4e-126
     Identities = 220/464 (47%), Positives = 317/464 (68%), Gaps = 5/464 (1%)

gi|74419984|gb|ABA04183.1|  homospermidine synthase [Nitrobacter winogradskyi Nb-255]
     Length=478
     Score =  432 bits (1112),  Expect = 1e-119
     Identities = 217/464 (46%), Positives = 299/464 (64%), Gaps = 8/464 (1%)








 
Structural Features:
pfam06408, Homo_sperm_syn, Homospermidine synthase. This family consists of several 
homospermidine synthase proteins (EC:2.5.1.44). Homospermidine synthase (HSS) catalyses the 
synthesis of the polyamine homospermidine from 2 mol putrescine in an NAD(+)-dependent reaction.
             CD-Length = 471 residues,  99.4% aligned
             Score =  496 bits (1279), Expect = 2e-141






   
Genomic Context:






   
Comment:


















    ReferenceID:11791




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:29008




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:5944
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
197984
                           gb|AAP94695.1|
                           (AY258138) putative homospermidine synthase [Pseudomonas aeruginosa]
                           469
                           99.99
                           99.99
                           0
                           2460.0
     

     
     
197988
                           gb|AAB63957.1|
                           (L77975) homospermidine synthase [Blastochloris viridis]
                           483
                           45.13
                           63.35
                           20
                           1074.0
     

     
     
197985
                           emb|CAE26309.1|
                           (BX572595) homospermidine synthase [Rhodopseudomonas palustris CGA009]
                           481
                           45.11
                           62.78
                           17
                           1114.0
     

     
     
197987
                           dbj|BAC53027.1|
                           (AP005963) homospermidine synthase [Bradyrhizobium japonicum USDA 110]
                           485
                           44.94
                           61.64
                           21
                           1093.0
     

     
     
197989
                           gb|AAO55386.1|
                           (AE016862) homospermidine synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           479
                           44.67
                           62.00
                           19
                           1060.0
     

     






      

          
GC ORFID: 48946How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 1769329Stop: 1770738

          
Length: 1410


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: Homolog Bit Score 

          
Other Homologs:  

          
GC ORF Sequence

           
ATGGATTTTTCAATTAATCCCCCCCAGCGAATTGTCTTTGTGGGACTAGGAACGATTGCCCAGTCCTTCCTT 
CCTCTCCTTTCTAAGGTTCACGACTTATCTACTTTAGAAATCTACGCGATCGATCCAAAAACACCACCGCTT 
ATTGAGTACTTTGCTAACAGTTTTGGCTTAAAATTTATCAACTCTGCAATTGATCAAATCAACTATCGCGAC 
ATTCTTGTGCCGATTCTTGGTGAAGGGACGGTTCTAATAAATCTATCAACCGATGTCTCAAGTCTTGCCCTG 
ATAGAATTATGCCGCTCCGCAGGGGCTCTTTATTTAGACACATGCATTGAGCCGTGGAAGGGCGGATATGAT 
GACCCAACCATACCACTGCATAAGCGAACTAACTATCACTTAAGAGAACAAATGCTGTCTCTGAAAAAGAGG 
CTAGGCTCAGGGGTGACGGCTCTCGTGGCGCATGGCGCGAATCCTGGGTTGGTATCCCACTTCGTTAAACGT 
GCTCTTTTAGATTTAGCGGAAGAGATATTGGGTGATTGTAAAAAGCCCTCTAATAAAGAGCAGTGGGCTATC 
CTAAGCCAAAGATTGGGTGTCAAAGTAATCCATGTCGCAGAGTACGACTCGCAAATTTCTCAGAAATCTAGG 
GAGAGGGGTGAGTTCGTAAATACTTGGTCAGTGCATGGGTTCATAAGTGAGTCGCAACAACCGGCAGAGTTG 
GGCTGGGGAAGTCACGAGCGAAGTCTACCCACGGATGCGTCAATGCATACAGATGGATGCGGAGCAGCAATC 
TATATTGAGAAGCCAGGGGCCTCTGTTAGAGTTAAGACATGGACGCCATTTAACGGCCCTAGCTTAGGTTAT 
TTGGTAACTCATCACGAGGCAATTTCAATTGCTGACTTTTTGACGCTCAGAACTGCCGATGAGACATATAGA 
CCAACTGTCCATTATGCCTATCGCCCAAGCGATGAAGCAATTCTCTCTGTACATGAATGGTTCGGCAATGAT 
TGCATGACTCCAGAAAAGACAAAAGTACTTCGTCCTGGTGATATCTTGTCGGGTTCTGATTACCTCGGTGTT 
CTTCTTATGGGGCACGAAAAATCTAGTTATTGGTATGGATCTATCTTAAGCATCGAAAAGGCCAAGGAACTT 
GCCACTCTCAACACTGCAACAACGTTACAGGTAGCAGCCGGAGTTCTTTCCGGTTACTTATGGATCCTGAGC 
CATCCGTCGGCAGGAATAATTGAAGCCGAAGACATGGATCATGAAGTGGCACTATCTTATATTTCACAGTAT 
CTAGGCGAACTAAAAGGGGTTTATTCGGACTGGAATCCTACCAAAAACAACCCAGGCACCTTTTCGGCCATA 
GATAGCGACTCTCCCTGGCTGTTCTCAAATTTTGTCCTCTGA