ViewGene
Locus Name:
GeneID:
Location:

GeneID:591
TrivialName: PA14_35190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35190
PAO1 Ortholog Locus:PA2272
Sequence Length:1698
Protein Length:565
Start: 3131228
Stop: 3129531
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05660.1|AE004653_3 (AE004653) penicillin-binding protein 3A [Pseudomonas aeruginosa PAO1]
Identities = 564/565 (99%), Positives = 564/565 (99%)
Sequence:
ATGAGCAGTCAACGCCGAAACTACCGCTTCATCCTTGTCGTCACCCTGTTCGTCCTCGCCTCCCTGGCCGTC
TCCGGACGGTTGGTCTATCTCCAGGTCCACGACCACGAATTCCTCGCCGACCAGGGCGACCTCCGCTCGATC
CGCGACCTACCGATCCCGGTCACCCGCGGCATGATCACCGACCGCAACGGCGAGCCGCTGGCGGTATCCACC
GAAGTCGCGTCGATCTGGTGCAACCCCAGGGAAATGGCCGCCCACCTCGACGAGGTGCCACGCCTGGCCGGC
GCCCTGCACCGCCCCGCGGCGCCGCTGCTGGCCCAGCTCCAGGCCAACCCGAACAAGCGCTTCCTCTACCTC
GAGCGCGGCCTGTCGCCGATCGAGGCCAGCGAGGTGATGGCCCTGGGCATAACGGGGGTACACCAGATCAAG
GAATACAAGCGTTTCTACCCCAGTTCCGAGCTGACCGCGCAGTTGATCGGCCTGGTCAACATCGACGGCCGC
GGCCAGGAAGGCACCGAACTGGGCTTCAACGACTGGCTGAGCGGCAAGGACGGAGTACGCGAGGTGGCGATC
AACCCGCGCGGCTCGCTGGTCAACAGCATCAAGGTGCTGAAGACGCCCAAGGCCAGCCAGGACGTGGCCCTG
AGCATCGACCTGCGACTACAGTTCATCGCCTACAAGGCGCTGGAAAAGGCCGTGCTCAAGTTCGGCGCACAC
TCCGGCTCGGCGGTCCTGGTGAACCCGAAGAGCGGGCAGATCCTGGCGATGGCCAACTTCCCCTCCTACAAC
CCGAACAACCGCGCCAGCTTCGCCCCGGCCTTCATGCGCAACCGCACCCTCACCGATACCTTCGAGCCGGGC
TCGGTGATCAAGCCGTTCAGCATGTCGGCGGCGCTGGCCTCCGGCAAGTTCGACGAGAACAGCCAAGTCAGC
GTGGCACCGGGCTGGATGACCATCGACGGGCACACCATCCACGACGTCGCCCGGCGCGACGTACTGACCATG
ACCGGGGTGCTGATCAACTCCTCGAACATCGGCATGAGCAAGGTCGCCCTGCAGATCGGACCCAAGCCGATC
CTCGAACAGCTCGGCCGGGTCGGTTTCGGCGCGCCGCTGTCGCTGGGCTTCCCCGGCGAGAACCCGGGCTAC
CTGCCGTTCCACGAGAAATGGTCGAACATCGCCACCGCCAGCATGTCGTTCGGCTACAGCCTGGCGGTGAAC
ACCGCCGAGCTGGCCCAGGCCTACTCGGTGTTCGCCAACGACGGCAAGCTGGTGCCGCTCAGCCTGCTCCGC
GACAACCCGCAGAACCAGGTGCGCCAGGCGATGGACCCGCAGATCGCACGGCGCATCCGGGCGATGCTGCAA
ACCGTGGTAGAAGACCCGAAGGGCGTGGTCCGCGCCCGCGTGCCGGGCTACCACGTGGCGGGCAAGAGCGGC
ACCGCGCGCAAGGCCTCGGGCCGGGGCTACGCGGACAAGTCCTACCGTTCGCTGTTCGTCGGCATGGCGCCG
GCGTCCGACCCGCAACTGGTGCTGGCGGTGATGATCGATTCGCCGACCAGGATTGGCTACTTCGGCGGCCTG
GTCTCGGCGCCCACCTTCAGCGACATCATGGCCGGATCGCTACGCGCCCTGGCGATCCCGCCGGACAACCTG
CAGGACAGCCCGGCCGTCGCCGACCGCCAGCACCACGGCTGA
Translation:
MSSQRRNYRFILVVTLFVLASLAVSGRLVYLQVHDHEFLADQGDLRSIRDLPIPVTRGMITDRNGEPLAVST
EVASIWCNPREMAAHLDEVPRLAGALHRPAAPLLAQLQANPNKRFLYLERGLSPIEASEVMALGITGVHQIK
EYKRFYPSSELTAQLIGLVNIDGRGQEGTELGFNDWLSGKDGVREVAINPRGSLVNSIKVLKTPKASQDVAL
SIDLRLQFIAYKALEKAVLKFGAHSGSAVLVNPKSGQILAMANFPSYNPNNRASFAPAFMRNRTLTDTFEPG
SVIKPFSMSAALASGKFDENSQVSVAPGWMTIDGHTIHDVARRDVLTMTGVLINSSNIGMSKVALQIGPKPI
LEQLGRVGFGAPLSLGFPGENPGYLPFHEKWSNIATASMSFGYSLAVNTAELAQAYSVFANDGKLVPLSLLR
DNPQNQVRQAMDPQIARRIRAMLQTVVEDPKGVVRARVPGYHVAGKSGTARKASGRGYADKSYRSLFVGMAP
ASDPQLVLAVMIDSPTRIGYFGGLVSAPTFSDIMAGSLRALAIPPDNLQDSPAVADRQHHG*
AnnotationID:600GeneID:591
AnnotatorUID: diggins
Modification Date/Time: 2005-03-04 09:09:31
Gene Name:pbpC
Confidence Code:1
GeneProduct:penicillin-binding protein 3A
Cell Localization:()
Synonyms:ftsI
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0768,pfam00905,pfam03717
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66954.1|AE016324_4 (AE016779) penicillin-binding protein 3 [Pseudomonas putida KT2440]
          Length = 582

 Score =  543 bits (1400), Expect = e-154
 Identities = 267/549 (48%), Positives = 379/549 (69%), Gaps = 4/549 (0%)

>gb|AAO57863.1| (AE016871) penicillin-binding protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 575

 Score =  541 bits (1393), Expect = e-153
 Identities = 267/549 (48%), Positives = 378/549 (68%), Gaps = 4/549 (0%)

>gb|AAM35662.1| (AE011708) penicillin-binding protein 3 [Xanthomonas axonopodis pv.
           citri str. 306]
          Length = 622

 Score =  456 bits (1172), Expect = e-127
 Identities = 230/546 (42%), Positives = 347/546 (63%), Gaps = 3/546 (0%)








 
Structural Features:
COG0768, FtsI, Cell division protein FtsI/penicillin-binding protein 2 
[Cell envelope biogenesis, outer membrane]
CD-Length = 599 residues,  98.0% aligned
Score =  449 bits (1157), Expect = 3e-127
Identities = 215/592 (36%), Positives = 309/592 (52%), Gaps = 50/592 (8%)

pfam00905, Transpeptidase, Penicillin binding protein transpeptidase domain.
CD-Length = 295 residues, 100.0% aligned
Score =  254 bits (651), Expect = 2e-68
Identities = 114/307 (37%), Positives = 166/307 (54%), Gaps = 21/307 (6%)

pfam03717, PBP_dimer, Penicillin-binding Protein dimerisation domain. 
CD-Length = 166 residues, 100.0% aligned
Score =  124 bits (313), Expect = 2e-29
Identities = 61/166 (36%), Positives = 90/166 (54%), Gaps = 14/166 (8%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1185




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18327




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32508




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:591_cdd.html


    		




 
URL:

















    ReferenceID:34451




  
Author/Investigator(s):
        Liao X, Hancock RE.





  
Title:
        Identification of a penicillin-binding protein 3 homolog, PBP3x, in Pseudomonas aeruginosa: gene cloning and growth phase-dependent expression.




  
PubMed:
        9045804


MedLine:
        




    
Source:
J Bacteriol. 1997 Mar;179(5):1490-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9045804

















    ReferenceID:34452




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173









           
Homologs By Global Alignment 

           

       



     
Gene ID:591
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1011
                           PA2272_tr
                           translation of PA2272
                           565
                           99.82
                           99.82
                           0
                           2865.0
     

     
     
39686
                           gb|AAG05660.1|AE004653_3
                           (AE004653) penicillin-binding protein 3A [Pseudomonas aeruginosa PAO1]
                           565
                           99.82
                           99.82
                           0
                           2865.0
     

     
     
39687
                           emb|CAA64770.1|
                           (X95517) penicillin-binding protein 3A [Pseudomonas aeruginosa]
                           565
                           99.64
                           99.82
                           0
                           2862.0
     

     
     
39691
                           gb|AAO57863.1|
                           (AE016871) penicillin-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           575
                           47.13
                           66.95
                           10
                           1390.0
     

     
     
39689
                           gb|AAG07806.1|AE004856_17
                           (AE004856) penicillin-binding protein 3 [Pseudomonas aeruginosa PAO1]
                           585
                           46.83
                           65.29
                           26
                           1398.0
     

     






      

          
GC ORFID: 43593How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2272Glimmer Score: 

          
Start: 1822688Stop: 1824385

          
Length: 1698


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2272 translation of PA2272Homolog Bit Score 1108.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2040 (ORF: BLASTX 546.0), t_PA2272 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAGTCAACGCCGAAACTACCGCTTCATCCTTGTCGTCACCCTGTTCGTCCTCGCCTCCCTGGCCGTC 
TCCGGACGGTTGGTCTATCTCCAGGTCCACGACCACGAATTCCTCGCCGACCAGGGCGACCTCCGCTCGATC 
CGCGACCTACCGATCCCGGTCACCCGCGGCATGATCACCGACCGCAACGGCGAGCCGCTGGCGGTATCCACC 
GAAGTCGCGTCGATCTGGTGCAACCCCAGGGAAATGGCCGCCCACCTCGACGAGGTGCCACGCCTGGCCGGC 
GCCCTGCACCGCCCCGCGGCGCCGCTGCTGGCCCAGCTCCAGGCCAACCCGAACAAGCGCTTCCTCTACCTC 
GAGCGCGGCCTGTCGCCGATCGAGGCCAGCGAGGTGATGGCCCTGGGCATAACGGGGGTACACCAGATCAAG 
GAATACAAGCGTTTCTACCCCAGTTCCGAGCTGACCGCGCAGTTGATCGGCCTGGTCAACATCGACGGCCGC 
GGCCAGGAAGGCACCGAACTGGGCTTCAACGACTGGCTGAGCGGCAAGGACGGAGTACGCGAGGTGGCGATC 
AACCCGCGCGGCTCGCTGGTCAACAGCATCAAGGTGCTGAAGACGCCCAAGGCCAGCCAGGACGTGGCCCTG 
AGCATCGACCTGCGACTACAGTTCATCGCCTACAAGGCGCTGGAAAAGGCCGTGCTCAAGTTCGGCGCACAC 
TCCGGCTCGGCGGTCCTGGTGAACCCGAAGAGCGGGCAGATCCTGGCGATGGCCAACTTCCCCTCCTACAAC 
CCGAACAACCGCGCCAGCTTCGCCCCGGCCTTCATGCGCAACCGCACCCTCACCGATACCTTCGAGCCGGGC 
TCGGTGATCAAGCCGTTCAGCATGTCGGCGGCGCTGGCCTCCGGCAAGTTCGACGAGAACAGCCAAGTCAGC 
GTGGCACCGGGCTGGATGACCATCGACGGGCACACCATCCACGACGTCGCCCGGCGCGACGTACTGACCATG 
ACCGGGGTGCTGATCAACTCCTCGAACATCGGCATGAGCAAGGTCGCCCTGCAGATCGGACCCAAGCCGATC 
CTCGAACAGCTCGGCCGGGTCGGTTTCGGCGCGCCGCTGTCGCTGGGCTTCCCCGGCGAGAACCCGGGCTAC 
CTGCCGTTCCACGAGAAATGGTCGAACATCGCCACCGCCAGCATGTCGTTCGGCTACAGCCTGGCGGTGAAC 
ACCGCCGAGCTGGCCCAGGCCTACTCGGTGTTCGCCAACGACGGCAAGCTGGTGCCGCTCAGCCTGCTCCGC 
GACAACCCGCAGAACCAGGTGCGCCAGGCGATGGACCCGCAGATCGCACGGCGCATCCGGGCGATGCTGCAA 
ACCGTGGTAGAAGACCCGAAGGGCGTGGTCCGCGCCCGCGTGCCGGGCTACCACGTGGCGGGCAAGAGCGGC 
ACCGCGCGCAAGGCCTCGGGCCGGGGCTACGCGGACAAGTCCTACCGTTCGCTGTTCGTCGGCATGGCGCCG 
GCGTCCGACCCGCAACTGGTGCTGGCGGTGATGATCGATTCGCCGACCAGGATTGGCTACTTCGGCGGCCTG 
GTCTCGGCGCCCACCTTCAGCGACATCATGGCCGGATCGCTACGCGCCCTGGCGATCCCGCCGGACAACCTG 
CAGGACAGCCCGGCCGTCGCCGACCGCCAGCACCACGGCTGA